Selektion in Gewebekulturen mit haploiden Zellen

In vitro selection and regeneration of carnation (Dianthus caryophyllus L.) plants resistant to culture filtrate of Fusarium oxysporum f.sp. dianthi

“…A number of previous studies have also found that the toxin present in the CF was able to inhibit cell growth and that the cells of the host species were more sensitive to the toxin than those of non-host plants (Hartman et al 1984;Selvapandiyan et al 1988;Malepszy and El Kazzaz 1990). Binding et al (1970) was the first to demonstrate the possibility of in vitro selection of callus cultures for desirable traits. Many attempts have been made since then to apply such selection schemes for crop improvement, including resistance to Fusarium wilt (Carlson 1973;Behnke 1980;Selvapandiyan et al 1988;Arai and Takeuchi 1993;Scala et al 1998;Venkatachalam et al 1998;Mosquera et al 1999).…”

Natural and induced variation in tissue culture

“…Plant cells in culture have demonstrated biochemical sensitivities similar to that of microbial genetic studies. For instance, isolation of artificially produced and spontaneous mutant cells in culture have yielded strains of carrot resistant to acriflavine (BLAKELY & STEWARD, 1964); tomato roots which tolerate low pyridoxine levels (WILLEMOT & BOLL, 1959); streptomycin resistant cells of haploid Petunia (BINDING, 1972;BINDING et al, 1970) and plants of tobacco (MALIGA et al, 1973b); and 8-azaguanine resistance has also been reported in tobacco (LESCURE, 1973). The ability of plant cells to grow in normally inhibitory levels of various amino acid analogs (CHALEFF & CARLSON, 1975 ;PALMER & WIDHOLM, 1975 ;WIDHOLM, 1972aWIDHOLM, , 1972bWIDHOLM, , 1974aWIDHOLM, , 1974bZENK, 1974) has yielded strains of cells which vary in their content of various nutritionally important amino acids.…”

Application of in vivo and in vitro mutation techniques for crop improvement

“…They drew attention to the use of cell culture for mutant selection, the ease of large scale screening of cell populations and the convenience of mutant induction and selection using haploid cell populations . Several papers confirmed the use of this method (Binding et al ., 1970 ;Carlson, 1973 ;Maliga, 1984) . Since then, several in vitro selected 3 1 1 variants have been investigated for their value in plant improvement .…”