In vitro selection and regeneration of carnation (Dianthus caryophyllus L.) plants resistant to culture filtrate of Fusarium oxysporum f.sp. dianthi

Abstract

Callus cultures derived from internodal segments of two cultivars of carnation susceptible to Fusarium oxysporum f.sp. dianthi were successfully used for in vitro selection for resistance to this pathogenic fungus. Resistant lines were selected by culturing calli on growth medium containing various concentrations of the culture filtrate of F. oxysporum f.sp. dianthi. Resistant calli obtained after two cycles (25 days/cycle) of selection were used for plant regeneration. About 32% of the plants regenerated from the resistant calli had acquired considerable resistance against the pathogen in the field. No phenotypic variation was observed in the selected regenerates.

In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt

“…This is because the plants are grown in a setting that more closely resembles actual field conditions, allowing for a complete plant-pathogen interaction (Predieri 2001). A strong correlation between in vitro selection and in vivo resistance in other host-parasite interactions has been reported (Thakur et al 2002;Kumar et al 2008;Wilson Fig. 5 Effect of the Fusarium oxysporum f. sp.…”

“…In the later case, selection can be advantageous since resistant plants can be selected during initial growth stages employing toxins as in vitro selective agents, which drastically reduces the number of selection cycles made by crop improvement programs (Ravikumar et al 2007). Sometimes, a high correlation between in vitro and in vivo resistance to pathogenic fungi has been found by the regeneration capability of calli in the presence of culture filtrates (Thakur et al 2002;Kumar et al 2008).…”

Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes

“…The best-studied biotic stresses have been fungal diseases, using toxins or filtrate culture as selective agents (Svabova & Lebeda, 2005). In vitro selection resulted in the isolation of resistant lines in carnation to Fusarium oxysporum f. sp dianthi (Thakur et al, 2002), in strawberry to Alternaria alternata (Takahashi et al, 1992), and in wheat to Fusarium graminearum (Ahmed et al, 1996). Salinity is the main abiotic stress that has been addressed by in vitro selection (Flowers, 2004;Zair et al, 2003), although applications to other stresses, such as zinc tolerance, have also been reported (Samantaray et al, 1999).…”

In vitro selection and regeneration of chrysanthemum (Dendranthema grandiflorum Tzelev) plants resistant to culture filtrate of Septoria obesa Syd

“…In order to screen for target characters in a large number of mutagenized cells in vitro, it is essential to have efficient selection agents. Toxic culture filtrate and purified toxins have been used for in vitro selection of disease resistant plants (Thakur et al 2002;Liu et al 2005). Tissue culture involves the selection of callus culture in vitro, taking advantages of the vast somaclonal variations (Skirvin 1978;Scowcroft and Larkin 1982).…”