Seleksi dan Optimasi Karakter Fisik Bakteri Penghasil Fitase yang Diisolasi dari Sumber Air Panas di Guci, Tegal

Abstract

<p>The aimed of this study to isolate, select and characterize physically phytase-producing bacteria<br />from hot springs in the Guci, Tegal. Isolation and enrichment of bacteria using Lurya Betani media<br />with 2% of phytic acid substrate. Extraction of extracellular phytase thus obtained extract coarse and<br />optimization by loking at the relative activity at the difference in the resulting product. Phytaseproducing<br />bacteria can be isolated from the hot springs of the Guci, Tegal with the highest relative<br />activity in AG2 and AG2-1 colonies. The highest relative activity at a temperature of 55°C, pH 6, the<br />incubation time of 90 minutes, the substrate concentration of 3% and a metal cofactor Ca2+ at a<br />concentration of 10-4M. Phytase-producing bacteria can be isolated from a hot spring in Guci, Tegal<br />and have certain physical characteristics.</p>

Isolasi, Seleksi Dan Optimasi Bakteri Penghasil Fitase Dari Tanah Vulkanik Di Guci, Tegal

“…Isolat dari area tanah vulkanik di Selo, Jawa Tengah dan Cangkringan, Yogyakarta, Indonesia mampu menghasilkan fitase ekstraseluler yang mampu mendegradasi asam fitat (Sajidan et al, 2015). Bakteri penghasil fitase dapat diisolasi dan dikarakterisasi dari sumber air panas dari Guci, Tegal (Purwati et al, 2016).…”