Selective transfection with osmotically active sorbitol modified PEI nanoparticles for enhanced anti-cancer gene therapy

MicroRNA delivery with osmotic polysorbitol-based transporter suppresses breast cancer cell proliferation

“…A major reason may be that PSMT interaction with the cells is predominantly through a caveolae-mediated pathway. Caveolae proteins are also overexpressed in cancer cells relative to normal cells [16]. Targeted delivery of this carrier may therefore be more cyto friendly and may also be more effective therapeutically.…”

Cell Transfection-A Short Approach of DNA Mutagenesis

“…20 Liposomes formed by cationic lipids: The term "liposome" refers to lipid bi-layers that forms colloidal particles in an aqueous medium. 21,22 Artificial liposomes were being used to deliver DNA into cells, 23 the advances in liposomal vehicles were development of synthetic cationic lipids by Felgner et al 24 Liposome-mediated delivery offers advantages such as relatively high efficiency of gene transfers, ability to transfect certain cell types that are resistant to calcium phosphate or DEAE-dextran, in vitro and in vivo applications, successful delivery of DNA of all sizes of oligonucleotides at yeast artificial chromosomes, [24][25][26][27][28][29][30] delivery RNA 31,32 and protein. 33 While DNA will need to enter the nucleus, the cytoplasm is the site of action for RNA, protein or antisense oligonucleotides delivered via liposomes.…”

“…29 However, the polysorbitol mediated transporter-PSMT carrier forms a cover having polysorbitol osmotic property, then when the vehicle binds to the cells and the cells by osmosis through the particles absorb proteins called caveolae. 30 The transfection detection could be measured by the intensity of cell transgene expression and quantifying the activity of luciferase by a multi-plate luminometer. Transfection activity is expressed as relative light units (RLU) per milligram of cell protein.…”

Multifunctional Delivery Systems for Cancer Gene Therapy

“…Nguyen et al reported that a polysorbitolmediated transporter (PSMT) was used to deliver plasmid DNA encoding the p53 tumour suppressor gene into human cervical cancer (HeLa) cells and normal human diploid fibroblast (HDF) cells. PSMT entered cancer cells selectively via CvME with transgene expression resulting in cellular damage and apoptosis [49].…”