Cell Transfection-A Short Approach of DNA Mutagenesis

Abstract

The transfection method could be used for a variety of applications: large scale production of proteins of biological interest, inserting genes into eukaryotic cells to these acquires desired characteristics; analyze maintenance of genes and their expression; and future application in vivo as vaccines DNA and gene therapy. In order to improve and establish new conditions, as well as verify the most current techniques of cell transfection, this review aims to bring together the most current methods in order to be applied to the study and development of new techniques, always looking for the improving from limitations of existing techniques, afterward to be choose more appropriate methods for the required goals.