Selective spectrofluorimetric method for determination of Lisinopril in pharmaceutical preparations and in presence of hydrochlorothiazide: Application to content uniformity testing

Abstract

A novel sensitive and cost-effective spectrofluorimetric method has been developed and validated for determination of lisinopril (an angiotensin converting enzyme inhibitor) in its pure form and pharmaceutical preparations. The method is based on the reaction of the drug with ninhydrin and phenylacetaldehyde in buffered medium (pH 7.0) to form a highly fluorescent product measured at 460 nm after excitation at 390 nm. Different experimental parameters were optimized and calibration curve was constructed. The fluorescence-concentration relationship was linear in the range of 0.15-4.0 μg mL . The calculated Limit of detection (LOD) and Limit of quantitation (LOQ) were 0.04 and 0.12 μg mL , respectively. The method was successfully applied for the analysis of pharmaceutical preparations containing the studied drug either alone or co-formulated with hydrochlorothiazide. The obtained results were in agreement with those of the reported method in respect to accuracy and precession. Moreover, the method was applied content uniformity testing according to United States Pharmacopeia (USP) guidelines.

Switch on fluorescence probe for the selective determination of lisinopril in pharmaceutical formulations: application to content uniformity testing

“…The oral dosage of lisinopril ranges from 5 to 40 mg daily (single or divided dosage), with 5 and 10 mg daily being appropriate for the initiation of therapy for heart failure and hypertension, respectively. 1 Different analytical methods have been developed for determination of lisinopril including; spectrophotometric 2-10 spectrouorimetric, [10][11][12][13][14][15] polarographic, 7 chromatographic, 4,9,[16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] capillary electrophoretic [32][33][34][35] ow injection 36 and immunoassay 37 methods.…”

“…However, most of the reported spectrouorimetric methods include heating, which is a time consuming step. [10][11][12][13][14][15] Fluorescamine reacts readily with primary amino groups to form highly uorescent compounds, even though uorescamine itself is nonuorescent. These properties make uorescamine ideal for detecting amino groups, especially in proteins, peptides, and amino acids.…”

Utility of ninhydrin reagent for spectrofluorimetric determination of heptaminol in human plasma

“…Ninhydrin and an aldehyde were widely used as a reagent that reacts with primary amines to give colored fluorescent derivatives (Fig. ) . The use of phenylacetaldehyde together with ninhydrin showed a higher increase in fluorescence intensity of the product than the use of any other aldehyde .…”

Determination of Lisinopril in Pure and Tablet form by Using 2-Hydroxynaphthaldehyde as Derivatizing Reagent

“…The literature reveals various analytical methods were described for the determination of LNP such as titrimetric [5,6], Spectrophotometric [7][8][9][10][11][12][13][14][15], spectrofluorometric [16][17][18][19][20], chromatographic [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38], derivative UV-spectrophotometric, [39][40] polarography [41][42], radioimmunoassay [43] and fluoroimmunoassay [44].…”