Selective Separation of Similarly Sized Proteins with Tunable Nanoporous Block Copolymer Membranes

Qiu, Xiaoyan; Yu, Haizhou; Peinemann, Klaus‐Viktor

doi:10.1016/j.proeng.2012.08.450

Cited by 46 publications

(68 citation statements)

References 7 publications

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“…The carboxylic acid units were deprotonated at high pH and the PAA segments stretched to minimize the charge repulsion, transforming the pore into a pH-sensitive gate without affecting the overall spherical shape of the particle. The effect is opposite to that observed in recently reported stimuli-responsive PS-b-P4VP isoporous membranes 36,37 .…”

Section: Preparation and Characterization Of Nanoporous Particlescontrasting

confidence: 99%

Biomimetic block copolymer particles with gated nanopores and ultrahigh protein sorption capacity

et al. 2014

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The design of micro-or nanoparticles that can encapsulate sensitive molecules such as drugs, hormones, proteins or peptides is of increasing importance for applications in biotechnology and medicine. Examples are micelles, liposomes and vesicles. The tiny and, in most cases, hollow spheres are used as vehicles for transport and controlled administration of pharmaceutical drugs or nutrients. Here we report a simple strategy to fabricate microspheres by block copolymer self-assembly. The microsphere particles have monodispersed nanopores that can act as pH-responsive gates. They contain a highly porous internal structure, which is analogous to the Schwarz P structure. The internal porosity of the particles contributes to their high sorption capacity and sustained release behaviour. We successfully separated similarly sized proteins using these particles. The ease of particle fabrication by macrophase separation and self-assembly, and the robustness of the particles makes them ideal for sorption, separation, transport and sustained delivery of pharmaceutical substances.

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Section: Preparation and Characterization Of Nanoporous Particlescontrasting

confidence: 99%

Biomimetic block copolymer particles with gated nanopores and ultrahigh protein sorption capacity

et al. 2014

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“…As described in the introduction, the microphase separation of block copolymers in solvent mixtures can be advantageously used for the preparation of integral asymmetric membranes by SNIPS process [42,43,46,57]. In general, the pore structure formation is tremendously influenced by a variety of parameters such as, e.g., evaporation time, solvent mixture, additives like salts, humidity, temperature, etc.…”

Section: Membrane Preparation By Self-assembly and Non-solvent-inducementioning

confidence: 99%

“…In the last decade, functional block copolymer filtration membranes attracted enormous attention because of their isoporous surface structure and capability for responsiveness stimulated by external triggers [34][35][36][37][38][39][40][41]. The most prominent and first block copolymer in this field is polystyrene-block-poly(4-vinylpyridine) (PS-b-P4VP), which revealed tremendous potential for water purification and separation processes [42][43][44][45][46]. Quite recently, we reported on the preparation of the amphiphilic block copolymer polystyrene-block-poly(2-hydroxyethyl methacrylate) (PS-b-PHEMA) for the formation of integral asymmetric porous membranes featuring a high water flux [47].…”

Section: Introductionmentioning

confidence: 99%

Ferrocene-Modified Block Copolymers for the Preparation of Smart Porous Membranes

et al. 2017

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Abstract:The design of artificially generated channels featuring distinct remote-switchable functionalities is of critical importance for separation, transport control, and water filtration applications. Here, we focus on the preparation of block copolymers (BCPs) consisting of polystyrene-block-poly(2-hydroxyethyl methacrylate) (PS-b-PHEMA) having molar masses in the range of 91 to 124 kg mol −1 with a PHEMA content of 13 to 21 mol %. The BCPs can be conveniently functionalized with redox-active ferrocene moieties by a postmodification protocol for the hydrophilic PHEMA segments. Up to 66 mol % of the hydroxyl functionalities can be efficiently modified with the reversibly redox-responsive units. For the first time, the ferrocene-containing BCPs are shown to form nanoporous integral asymmetric membranes by self-assembly and application of the non-solvent-induced phase separation (SNIPS) process. Open porous structures are evidenced by scanning electron microscopy (SEM) and water flux measurements, while efficient redox-switching capabilities are investigated after chemical oxidation of the ferrocene moieties. As a result, the porous membranes reveal a tremendously increased polarity after oxidation as reflected by contact angle measurements. Additionally, the initial water flux of the ferrocene-containing membranes decreased after oxidizing the ferrocene moieties because of oxidation-induced pore swelling of the membrane.

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“…In the past years, nanopore-based device is attracting more and more attentions for its potential applications in selective molecular separation [1,2], biosensing [3,4], energy storage [5,6], controlled release [7,8], drug delivery [9,10], nanofluidics, and nanoelectronics [11][12][13][14]. It is generally believed that the premise of this method is how to obtain proper nanopores and how to design effective fluidic device.…”

Section: Introductionmentioning

confidence: 99%

Thermally modulated biomolecule transport through nanoconfined channels

Liu¹,

Zhu²

2016

Nano Online

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In this work, a nanofluidic device containing both a feed cell and a permeation cell linked by nanopore arrays has been fabricated, which is employed to investigate thermally controlled biomolecular transporting properties through confined nanochannels. The ionic currents modulated by the translocations of goat antibody to human immunoglobulin G (IgG) or bovine serum albumin (BSA) are recorded and analyzed. The results suggest that the modulation effect decreases with the electrolyte concentration increasing, while the effects generated by IgG translocation are more significant than that generated by BSA translocation. More importantly, there is a maximum decreasing value in each modulated current curve with biomolecule concentration increasing for thermally induced intermolecular collision. Furthermore, the turning point for the maximum shifts to lower biomolecule concentrations with the system temperature rising (from 4°C to 45°C), and it is mainly determined by the temperature in the feed cell if the temperature difference exists in the two separated cells. These findings are expected to be valuable for the future design of novel sensing device based on nanopore and/or nanopore arrays.

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Selective Separation of Similarly Sized Proteins with Tunable Nanoporous Block Copolymer Membranes

Cited by 46 publications

References 7 publications

Biomimetic block copolymer particles with gated nanopores and ultrahigh protein sorption capacity

Biomimetic block copolymer particles with gated nanopores and ultrahigh protein sorption capacity

Ferrocene-Modified Block Copolymers for the Preparation of Smart Porous Membranes

Thermally modulated biomolecule transport through nanoconfined channels

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