Biomimetic block copolymer particles with gated nanopores and ultrahigh protein sorption capacity

Yu, Haizhou; Qiu, Xiaoyan; Nunes, Suzana Pereira; Peinemann, Klaus‐Viktor

doi:10.1038/ncomms5110

Cited by 137 publications

(153 citation statements)

References 52 publications

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“…S1). The incorporation of hydrophobic NI groups renders the derived HA amphiphilic, enabling the formation of GRVs in the aqueous solution (37,38). Moreover, NI provides an hypoxiasensitive element, which is expected to be bioreduced under hypoxic conditions (24,27).…”

Section: Resultsmentioning

confidence: 99%

Microneedle-array patches loaded with hypoxia-sensitive vesicles provide fast glucose-responsive insulin delivery

Zhang

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

699

551

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A glucose-responsive "closed-loop" insulin delivery system mimicking the function of pancreatic cells has tremendous potential to improve quality of life and health in diabetics. Here, we report a novel glucose-responsive insulin delivery device using a painless microneedle-array patch ("smart insulin patch") containing glucoseresponsive vesicles (GRVs; with an average diameter of 118 nm), which are loaded with insulin and glucose oxidase (GO x ) enzyme. The GRVs are self-assembled from hypoxia-sensitive hyaluronic acid (HS-HA) conjugated with 2-nitroimidazole (NI), a hydrophobic component that can be converted to hydrophilic 2-aminoimidazoles through bioreduction under hypoxic conditions. The local hypoxic microenvironment caused by the enzymatic oxidation of glucose in the hyperglycemic state promotes the reduction of HS-HA, which rapidly triggers the dissociation of vesicles and subsequent release of insulin. The smart insulin patch effectively regulated the blood glucose in a mouse model of chemically induced type 1 diabetes. The described work is the first demonstration, to our knowledge, of a synthetic glucose-responsive device using a hypoxia trigger for regulation of insulin release. The faster responsiveness of this approach holds promise in avoiding hyperglycemia and hypoglycemia if translated for human therapy.diabetes | drug delivery | glucose-responsive | hypoxia-sensitive | microneedle

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Section: Resultsmentioning

confidence: 99%

Microneedle-array patches loaded with hypoxia-sensitive vesicles provide fast glucose-responsive insulin delivery

Zhang

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

699

551

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“…Depending on the thermodynamic conditions, these particles can have high porosity. In block copolymer systems they can be characterized by exceptional order 63 .…”

Section: Non-solvent Induced (Macro) Phase Separation (Nips): Interplmentioning

confidence: 99%

Block Copolymer Membranes for Aqueous Solution Applications

2016

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Block copolymers are known for their intricate morphology. We review the state of the art of block copolymer membranes and discuss perspectives in this field. The main focus is on pore morphology tuning with a short introduction on non-porous membranes. The two main strategies for pore formation in block copolymer membranes are (i) film casting and selective block sacrifice and (ii) self-assembly and non-solvent induced phase separation (SNIPS). Different fundamental aspects involved in the manufacture of block copolymer membranes are considered, including factors affecting the equilibrium morphology in solid films, self-assembly of copolymer in solutions and macrophase separation by solvent-non-solvent exchange. Different mechanisms are proposed for different depths of the SNIPS membrane. Block copolymer membranes can be prepared with much narrower pore size distribution than homopolymer membranes. Open questions and indications of what we consider the next development steps are finally discussed. They include the synthesis and application of new copolymers and specific functionalization, adding characteristics to respond to stimuli and chemical environment, polymerization-induced phase separation, and the manufacture of organic-inorganic hybrids.3

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“…The bicontinuous cubic networks of large pores embedded within the IBC structure of the BCP bilayers could allow these porous polymer monoliths to be used as platforms for applications such Relative pressure P/P 0 as protein separation, biochemical transformation and nanotemplating 36,37 . To demonstrate the accessibility and usability of the internal networks of the water channels within the polymer monoliths, we introduced surface amino groups throughout the BCP bilayer by the co-assembly of 2 213 and NH 2 -PEG 45 -PS 210 , a linear BCP with an a-amino group at the hydrophilic PEG block under the SDEMS condition (2 213 /NH 2 -PEG 45 -PS 210 93:7 w/w in dioxane) 36 .…”

Section: Articlementioning

confidence: 99%

“…Solution self-assembly of BCPs has shown the formation of inverse mesophases, which resulted in the formation of colloidal nano-and microparticles having internal bicontinuous pore networks [32][33][34][35][36][37] . The direct self-assembly of BCPs into IBC structures consisting of the BCP bilayers is an emerging method to create highly regular bicontinuous porous polymers without any aid of post processes to generate internal pore networks and surface functional groups 36,37 .…”

mentioning

confidence: 99%

“…The direct self-assembly of BCPs into IBC structures consisting of the BCP bilayers is an emerging method to create highly regular bicontinuous porous polymers without any aid of post processes to generate internal pore networks and surface functional groups 36,37 . We recently showed that the dendritic-linear BCPs composed of a dendritic block with peripheral hydrophilic poly(ethylene glycol) (PEG) chains and a glassy polystyrene (PS) hydrophobic block (Fig.…”

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confidence: 99%

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Mesoporous monoliths of inverse bicontinuous cubic phases of block copolymer bilayers

Park

et al. 2015

Nat Commun

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Solution self-assembly of block copolymers into inverse bicontinuous cubic mesophases is a promising new approach for creating porous polymer films and monoliths with highly organized bicontinuous mesoporous networks. Here we report the direct self-assembly of block copolymers with branched hydrophilic blocks into large monoliths consisting of the inverse bicontinuous cubic structures of the block copolymer bilayer. We suggest a facile and scalable method of solution self-assembly by diffusion of water to the block copolymer solution, which results in the unperturbed formation of mesoporous monoliths with largepore (425 nm diameter) networks weaved in crystalline lattices. The surface functional groups of the internal large-pore networks are freely accessible for large guest molecules such as protein complexes of which the molecular weight exceeded 100 kDa. The internal double-diamond (Pn3m) networks of large pores within the mesoporous monoliths could be replicated to self-supporting three-dimensional skeletal structures of crystalline titania and mesoporous silica.

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Biomimetic block copolymer particles with gated nanopores and ultrahigh protein sorption capacity

Cited by 137 publications

References 52 publications

Microneedle-array patches loaded with hypoxia-sensitive vesicles provide fast glucose-responsive insulin delivery

Microneedle-array patches loaded with hypoxia-sensitive vesicles provide fast glucose-responsive insulin delivery

Block Copolymer Membranes for Aqueous Solution Applications

Mesoporous monoliths of inverse bicontinuous cubic phases of block copolymer bilayers

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