Selective preconcentration on a metal loaded silica precolum and on-line HPLC separation of catechol derivatives in a complex medium. Application to the analysis of catecholamines in urine

Optimum conditions for the separation of 9-fluorenylmethyl chloroformate derivatized catecholamines by HPLC are described; three catecholamines (noradrenaline, adrenaline and dopamine) and an internal standard (epinine) were separated in less than 20 minutes under isocratic conditions. This method is 17 to 350 times more sensitive than electrochemical detection, depending on the test compounds. It has been applied to the analysis of catecholamines in urine. The sample was extracted by a metal-loaded silica prior to separation

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Selective preconcentration on a metal loaded silica precolum and on-line HPLC separation of catechol derivatives in a complex medium. Application to the analysis of catecholamines in urine

Cited by 5 publications

References 9 publications

Comparison between phenylboronic acid and iron loaded silicas to selectively preconcentrate catecholamines on-line coupled with HPLC

Comparison between phenylboronic acid and iron loaded silicas to selectively preconcentrate catecholamines on-line coupled with HPLC

Extension of the application of the thermal in-situ reaction on NH2 layers to the detection of catecholamines in biological materials

HPLC separation of catecholamines after derivatization with 9-fluorenylmethyl chloroformate

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