Comparison between phenylboronic acid and iron loaded silicas to selectively preconcentrate catecholamines on-line coupled with HPLC

Coquet, A.; Descombes, A. A.; Veuthey, Jean‐Luc; Haerdi, W.

doi:10.1007/bf00323802

Cited by 5 publications

(1 citation statement)

References 20 publications

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“…As Mazzeo and Krull [2] summarized in their paper, there are quite a few materials based on boronate phase, including silica [3,4,10,17], polyacrylamide [5-7, 9,11,13,14], agarose [7,15,22], cellulose [6,15,17,21], polyacrylate [19], and porous glass [15]. A few fundamental conditions must be met in order for the material to be used in high performance ligand affinity chromatography (HPLAC) columns, First, the boronate phases must be stable to alkaline environments since alkaline eluents are used.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic Study of Immobilized Boronate Stationary Phase

Kitagawa¹,

Treat-Clemons²

1991

Anal. Sci.

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The properties of a new polymeric material with immobilized boronic acid for HPLAC columns are discussed. In order to achieve a high boronic acid content, a support polymer was chemically bonded to polyamines, then phenylboronic acid groups were reacted onto the multiple reaction sites. The resulting support material showed a binding capacity of more than 1 meq/mL. A 4,6 x 54 mm analytical column was used to separate bioamines, nucleosides, and glycated hemoglobin effectively.

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Section: Introductionmentioning

confidence: 99%

Chromatographic Study of Immobilized Boronate Stationary Phase

Kitagawa¹,

Treat-Clemons²

1991

Anal. Sci.

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HPLC separation of catecholamines after derivatization with 9-fluorenylmethyl chloroformate

Descombes

Haerdi

1992

Chromatographia

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Optimum conditions for the separation of 9-fluorenylmethyl chloroformate derivatized catecholamines by HPLC are described; three catecholamines (noradrenaline, adrenaline and dopamine) and an internal standard (epinine) were separated in less than 20 minutes under isocratic conditions. This method is 17 to 350 times more sensitive than electrochemical detection, depending on the test compounds. It has been applied to the analysis of catecholamines in urine. The sample was extracted by a metal-loaded silica prior to separation

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