Chromatographic Study of Immobilized Boronate Stationary Phase

Kitagawa, Naotaka; Treat-Clemons, Lynda

doi:10.2116/analsci.7.supple_195

Cited by 5 publications

(3 citation statements)

References 9 publications

(10 reference statements)

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“…PBAs with strong and/or specific interaction with diols were first developed to improve purification and analytical techniques. They were notably used in purification columns with the aim to isolate biomolecules, including monosaccharides, nucleosides, and glycoproteins. − These innovations inspired new technological developments for the sensing and release of small biological molecules, with application in drug delivery. ,− The more recent development of BE hydrogels has open a new era of research, with applications in local drug/cell delivery, 3D cell culture, and biofabrication.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Boronate Ester Hydrogels for Biomedical Applications: Challenges and Opportunities

Terriac,

Helesbeux,

Maugars

et al. 2024

Chem. Mater.

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Boronate ester (BE) hydrogels are increasingly used for biomedical applications. The dynamic nature of these molecular networks enables bond rearrangement, which is associated with viscoelasticity, injectability, printability, and selfhealing, among other properties. BEs are also sensitive to pH, redox reactions, and the presence of sugars, which is useful for the design of stimuli-responsive materials. Together, BE hydrogels are interesting scaffolds for use in drug delivery, 3D cell culture, and biofabrication. However, designing stable BE hydrogels at physiological pH (≈7.4) remains a challenge, which is hindering their development and biomedical application. In this context, advanced chemical insights into BE chemistry are being used to design new molecular solutions for material fabrication. This review article summarizes the state of the art in BE hydrogel design for biomedical applications with a focus on the materials chemistry of this class of materials. First, we discuss updated knowledge in BE chemistry including details on the molecular mechanisms associated with BE formation and breakage. Then, we discuss BE hydrogel formation at physiological pH, with an overview of the main systems reported to date along with new perspectives. A last section covers several prominent biomedical applications of BE hydrogels, including drug delivery, 3D cell culture, and bioprinting, with critical insights on the design relevance, limitations and potential.

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Section: Biomedical Applicationsmentioning

confidence: 99%

Boronate Ester Hydrogels for Biomedical Applications: Challenges and Opportunities

Terriac,

Helesbeux,

Maugars

et al. 2024

Chem. Mater.

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show abstract

“…This group of methods, known collectively as "boronate affinity chromatography", includes one of the earliest reported quantitative applications of affinity chromatography in the clinical laboratory-namely, the determination of glycohemoglobin for the assessment of long-term diabetes management ( Fig. 2) (7)(8)(9)(10)(11)(12)(13)(14)(15). At a pH above 8, most boronate derivatives form covalent bonds with compounds that contain cis-diol groups in their structure.…”

Section: Boronate Affinity Chromatographymentioning

confidence: 99%

“…After the initial report by Mallia et al, similar low-performance methods were reported or evaluated by other groups (10 -13 ). The same approach has since been adapted for use in HPAC and HPLC-based systems (9,11,14,15 ).…”

Section: Boronate Affinity Chromatographymentioning

confidence: 99%

Affinity Chromatography: A Review of Clinical Applications

Hage

1999

Clinical Chemistry

227

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Affinity chromatography is a type of liquid chromatography that makes use of biological-like interactions for the separation and specific analysis of sample components. This review describes the basic principles of affinity chromatography and examines its use in the testing of clinical samples, with an emphasis on HPLC-based methods. Some traditional applications of this approach include the use of boronate, lectin, protein A or protein G, and immunoaffinity supports for the direct quantification of solutes. Newer techniques that use antibody-based columns for on- or off-line sample extraction are examined in detail, as are methods that use affinity chromatography in combination with other analytical methods, such as reversed-phase liquid chromatography, gas chromatography, and capillary electrophoresis. Indirect analyte detection methods are also described in which immunoaffinity chromatography is used to perform flow-based immunoassays. Other applications that are reviewed include affinity-based chiral separations and the use of affinity chromatography for the study of drug or hormone interactions with binding proteins. Some areas of possible future developments are then considered, such as tandem affinity methods and the use of synthetic dyes, immobilized metal ions, molecular imprints, or aptamers as affinity ligands for clinical analytes.

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Pharmaceutical and biomedical applications of affinity chromatography: Recent trends and developments

Hage

Anguizola

et al. 2012

Journal of Pharmaceutical and Biomedical Analysis

177

127

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Affinity chromatography is a separation technique that has become increasingly important in work with biological samples and pharmaceutical agents. This method is based on the use of a biologically-related agent as a stationary phase to selectively retain analytes or to study biological interactions. This review discusses the basic principles behind affinity chromatography and examines recent developments that have occurred in the use of this method for biomedical and pharmaceutical analysis. Techniques based on traditional affinity supports are discussed, but an emphasis is placed on methods in which affinity columns are used as part of HPLC systems or in combination with other analytical methods. General formats for affinity chromatography that are considered include step elution schemes, weak affinity chromatography, affinity extraction and affinity depletion. Specific separation techniques that are examined include lectin affinity chromatography, boronate affinity chromatography, immunoaffinity chromatography, and immobilized metal ion affinity chromatography. Approaches for the study of biological interactions by affinity chromatography are also presented, such as the measurement of equilibrium constants, rate constants, or competition and displacement effects. In addition, related developments in the use of immobilized enzyme reactors, molecularly imprinted polymers, dye ligands and aptamers are briefly considered.

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Chromatographic Study of Immobilized Boronate Stationary Phase

Cited by 5 publications

References 9 publications

Boronate Ester Hydrogels for Biomedical Applications: Challenges and Opportunities

Boronate Ester Hydrogels for Biomedical Applications: Challenges and Opportunities

Affinity Chromatography: A Review of Clinical Applications

Pharmaceutical and biomedical applications of affinity chromatography: Recent trends and developments

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