Selective monitoring of vascular cell senescence via β-Galactosidase detection with a fluorescent chemosensor

Kim, Eun-Joong; Podder, Arup; Maiti, Mrinmoy; Lee, Jong Min; Chung, Bong Geun; Bhuniya, Sankarprasad

doi:10.1016/j.snb.2018.07.171

Cited by 35 publications

(15 citation statements)

References 32 publications

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“…Probe 66, for the detection of lysosomal β-Gal in senescent vascular endothelial cells, is composed of a rhodol skeleton fluorophore linked to galactose unit. [82] HEPES-DMSO 99.5:0.5 v/v solutions of 66 were almost non-emissive, whereas in the presence of β-Gal a marked emission at 545 nm was observed. Detection of endogenous β-Gal activity in HUVEC senescent cells (where senescence was induced by the treatment of normal HUVEC cells with H2O2) was achieved using probe 66 and confocal microscopy measurements.…”

Section: Chromo-fluorogenic Probes For Tracking β-Gal Activity In Vitromentioning

confidence: 93%

Chromo-fluorogenic probes for β-galactosidase detection

et al. 2021

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β-Galactosidase (β-Gal) is a widely used enzyme as a reporter gene in the field of molecular biology which hydrolyses the β-galactosides into monosaccharides. β-Gal is an essential enzyme in humans and its deficiency or its overexpression results in several rare diseases. Cellular senescence is probably one of the most relevant physiological disorders that involve β-Gal enzyme. In this review, we assess the progress made to date in the design of molecular-based probes for the detection of β-Gal both in vitro and in vivo. Most of the reported molecular probes for the detection of β-Gal consist of a galactopyranoside residue attached to a signalling unit through glycosidic bonds. The β-Gal-induced hydrolysis of the glycosidic bonds released the signalling unit with remarkable changes in colour and/or emission. Additional examples based on other approaches are also described. The wide applicability of these probes for the rapid and in situ detection of de-regulation β-Gal-related diseases has boosted the research in this fertile field. 33, 34 HBSS buffer 570/680 Turn on C6-lacZ 35 PBS (10 mM, pH 7.4) 405/545 Turn on C6-lacZ 36 HBSS buffer 640/680 Turn on HCT116-lacZ 37-39 HEPES (pH 7.3)-DMSO 99.9:0.01 v/v 555/582 Turn on COS-7-lacZ 40 PBS-EtOH/10:1 375/↓450↑5 40 Ratiometric (Twophoton) HEK293-lacZ 41 PBS 430/550 Turn on HeLa, HepG and HCT-116 + β-Gal (1 U) cell lines with high β-Gal activity per se 42 PBS 344/512 Turn on OVCAR-3 43 H2O-THF 99:1 333/495 Turn on (aggregates) OVCAR-3 44 PBS (20 mM, pH 4.6) 420/565 Turn on (aggregates) SKOV-3 45 PBS 488/560 Turn on (Twophoton) SKOV-3 46 PBS 350/↓450↑5 50 Ratiometric (Twophoton) OVCAR-3 47 PBS/DMSO 7:3 (50 mM, pH = 7.4) 460/650 Turn on (aggregates) OVCAR-3 48 PBS (10 mM, pH 7.4) 410/↓580↑6 50 Ratiometric OVCAR-3 49 PBS/DMSO 99/1 (0.05 M, pH 7.4)

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Section: Chromo-fluorogenic Probes For Tracking β-Gal Activity In Vitromentioning

confidence: 93%

Chromo-fluorogenic probes for β-galactosidase detection

et al. 2021

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“…Therefore, we established a cell senescence model by inducing H 2 O 2 and hydroxyurea (HU) according to the literature. [38][39][40][41] With the extension of incubation time, an increased fluorescence in both H 2 O 2 -and HU-incubated cells was observed (Figure 5e, f), indicating DSL-Gal can real-time tracking the senescence-associated β-gal in cells.…”

Section: Customization Of An Activatable Fluorescent Probe Dpl-gal For Sensing and Imaging Of β-Galmentioning

confidence: 99%

An Integration Strategy to Develop Dual-State Luminophores with Tunable Spectra, Large Stokes Shift, and Activatable Fluorescence for High-Contrast Imaging

Liu

Teng

et al. 2022

CCS Chem

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Developing dual-state luminophores (DSLs) with strong fluorescence both in monomer and aggregate state is of great necessary but still a huge challenge, because most of current luminophores are either aggregationinduced emission or aggregation-caused quenching molecules. Moreover, limited by the structural conservation of the few existing DSLs, there is not enough response sites that can be used to customize various activatable fluorescent probes for specific molecular imaging. Herein, we engineered a general integration strategy for fabricating such DSLs with excellent photophysical properties, eg. tunable spectra, large Stokes shift (> 170 nm), and achievable near-infrared emission, which shows great potential in high-contrast imaging.Importantly, the DSLs can be used as a universal platform for probe customization due to their activatable fluorescence through protection-deprotection of phenolic hydroxyl group. Based on this, a near-infrared fluorescent probe (DSL-Gal) was developed for sensing of β-galactosidase in solutions, senescent cells, and liver metastases with high contrast, further confirmed the superiority and universal feasibility of DSLs in probe design. The integration strategy may provide a novel approach for other DSLs generation and our DSLs may have great potential for bioimaging.

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“…Notably, KSL0401 displays a highly rapid response to β-gal (< 80 s) (Figure 10B), which is better than that of most previously reported SA-β-gal probes. Additionally, KSL0401 shows good biocompatibility and has been used to monitor SA-β-gal activity in various senescent 13) [35]. SRP displays faint fluorescence in spirocyclic form.…”

Section: Actamentioning

confidence: 99%

Small-molecule fluorescence-based probes for aging diagnosis

Shi

Liu

Wang

et al. 2022

Acta Materia Medica

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Aging is a time-dependent decline in physiological function that affects most organisms and is the major risk factor for many non-communicable diseases. The early diagnosis of aging is critical for the treatment of aging and aging-related diseases. In recent years, extensive efforts have attempted to accurately diagnose aging. To date, multiple types of fluorescent probes for various age-related biomarkers have been developed, with the aims of achieving rapid, precise diagnosis of the aging process and evaluating the efficacy of anti-aging drugs. This review summarizes recent research progress in small-molecule fluorescence-based probes for aging diagnosis, and further discusses the challenges and opportunities in this field.

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Selective monitoring of vascular cell senescence via β-Galactosidase detection with a fluorescent chemosensor

Cited by 35 publications

References 32 publications

Chromo-fluorogenic probes for β-galactosidase detection

Chromo-fluorogenic probes for β-galactosidase detection

An Integration Strategy to Develop Dual-State Luminophores with Tunable Spectra, Large Stokes Shift, and Activatable Fluorescence for High-Contrast Imaging

Small-molecule fluorescence-based probes for aging diagnosis

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