Selective molecularly imprinted polymer combined with restricted access material for in-tube SPME/UHPLC-MS/MS of parabens in breast milk samples

Determination of Parabens in Breast Milk Samples by Dispersive Liquid-Liquid Microextraction (DLLME) and Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry

“…The concentration range of parabens in breast milk determined in this study agreed with data of a previous study by our group, 9 conducted with 16 lactating mothers in Brazil. Using different sample preparation techniques, Rodríguez-Gómez et al 7,8,33,34 developed analytical methods and determined parabens in human milk samples with concentrations ranging from 0.1 to 7.5 ng mL -1 , 0.5 to 37.0 ng mL -1 , 0.6 to 43.5 ng mL -1 and 1.02 to 18.1 ng mL -1 , respectively.…”

“…9 The DLLME method described by Vela-Soria et al 37 to determine endocrine-disrupting chemicals in milk samples, presented lower LLOQ values. Probably, these results are related to the higher sensibility of the MS/MS detector.…”

“…18 Different sample preparation techniques combined with LC-MS/MS have been used to determine parabens in breast milk samples, including conventional techniques like online solid-phase extraction (online SPE), 10 molecularly imprinted micro-disc solid-phase extraction (MISPE), 11 stir-membrane solid-liquid-liquid microextraction (SM-SLLME), 8 in-tube solid-phase microextraction (in-tube SPME), 9 and quick, easy, cheap, effective, rugged and safe extraction (QuEChERS). 7 Rezaee et al 19 introduced the dispersive liquid-liquid microextraction (DLLME) in 2006.…”

“…Parabens can mimic estrogen action stimulating the growth of MCF7 cells, which are related to the development of breast cancer. 4,5 Parabens at trace levels have been determined in several biological matrices, such as urine, 2,6 breast milk, [7][8][9][10][11] plasma, 12 serum, 13 amniotic fluid, 14 and placental tissue. 15,16 As known, lipophilic substances are mainly excreted in breast milk, and consequently, that breast milk is the major way through which breastfed infants are exposed to environmental chemicals.…”

Desenvolvimento de fases extratoras seletivas para técnicas de microextração para a determinação de fármacos e endocanabinóide em amostras de plasma por LC-MS/MS

“…Recentemente, QUEIROZ e colaboradores desenvolveram um capilar com fase molecularmente impressa com revestimento hidrofílico (MIP-RAM) para sorção seletiva de parabenos e exclusão das macromoléculas de amostras de leite materno. Após a extração in-tube SPME, uma alíquota do extrato foi injetada no sistema LC-MS/MS (SOUZA et al, 2016).…”