Selective label-free electrochemical impedance measurement of glycated haemoglobin on 3-aminophenylboronic acid-modified eggshell membranes

Boonyasit, Yuwadee; Heiskanen, Arto; Chailapakul, O.; Laiwattanapaisal, Wanida

doi:10.1007/s00216-015-8680-8

Cited by 11 publications

(5 citation statements)

References 53 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Methods such as the chemiluminescence assay, radioactive immunoassay, enzyme linked immunosorbent assay (ELISA), and column chromatography have been used to detect insulin and HbA1c levels in buffer and serum. − For HbA1c the current gold standards are ion-exchange chromatography, boronated affinity chromatography, and electrophoresis . Based on the affinity of the diol groups of HbA1c to the boric acid surface, electrocatalytic detection of HbA1c on a boronate affinity modified surface, , a disposable amperometric sensor, and an impedimetric sensor on 3-aminophenylboronic acid-modified egg shell membranes have been demonstrated previously. All these prior assays/sensors had good sensitivity and selectivity, but they cannot offer real-time binding kinetics analysis with an image output of biomarker levels as in surface plasmon resonance (SPR) microarray imager.…”

mentioning

confidence: 99%

Magnetite-Quantum Dot Immunoarray for Plasmon-Coupled-Fluorescence Imaging of Blood Insulin and Glycated Hemoglobin

et al. 2017

View full text Add to dashboard Cite

New microarray chip strategies that are sensitive and selective and that can measure low levels of important biomarkers directly in a blood sample are significant for improving human health by allowing timely diagnosis of an abnormal condition. Herein, we designed an antibody–aptamer immunoarray chip to demonstrate simultaneous measurement of blood insulin and glycated hemoglobin (HbA1c) levels relevant to diabetic and prediabetic disorders using a surface plasmon microarray with validation by fluorescence imaging. To accomplish both surface plasmon and fluorescence imaging on the same sample, we decorated magnetite nanoparticles with quantum dots for covalent immobilization of aptamers for subsequent capture and isolation of the aptamers specific for insulin and HbA1c markers from 20-times diluted whole blood samples. Direct clinically relevant analysis, along with fluorescent imaging of the two markers, was achieved by this new immunoarray platform. The limit of detection was 4 pM for insulin and 1% for HbA1c. Examination of cross-talk using thrombin and platelet-derived growth factor confirmed that the designed immunoarray was highly selective for insulin and HbA1c. Surface plasmon kinetic analysis provided apparent binding constants of 0.24 (±0.08) nM and 37 (±3) μM, respectively, for the binding of insulin and HbA1c onto their surface immobilized monoclonal antibodies. Thus, quantitative imaging of ultralow levels of blood biomarker levels with binding kinetics is uniquely obtained in the designed immunoarray chip. In conclusion, this report demonstrates considerable significance of the developed magnetite-quantum dot-bioconjugate strategy for clinical diagnostics of whole blood biomarkers with characterization of molecular binding interactions.

show abstract

mentioning

confidence: 99%

Magnetite-Quantum Dot Immunoarray for Plasmon-Coupled-Fluorescence Imaging of Blood Insulin and Glycated Hemoglobin

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The hemolyzed sample was immobilized on the electrode surface and changes in the impedance were used for the label-free quantification of HbA1c between 2.3 and 14%. A ferro-ferricyanide couple was employed as the redox mediator in this study [ 41 ]. The study employed centrifugation to remove other glycated moieties that could potentially interfere with the analysis.…”

Section: Sensors For Quantification Of Hba1cmentioning

confidence: 99%

Trends in Quantification of HbA1c Using Electrochemical and Point-of-Care Analyzers

Mandali

Prabakaran

Annadurai

et al. 2023

Sensors

View full text Add to dashboard Cite

Glycated hemoglobin (HbA1c), one of the many variants of hemoglobin (Hb), serves as a standard biomarker of diabetes, as it assesses the long-term glycemic status of the individual for the previous 90–120 days. HbA1c levels in blood are stable and do not fluctuate when compared to the random blood glucose levels. The normal level of HbA1c is 4–6.0%, while concentrations > 6.5% denote diabetes. Conventionally, HbA1c is measured using techniques such as chromatography, spectroscopy, immunoassays, capillary electrophoresis, fluorometry, etc., that are time-consuming, expensive, and involve complex procedures and skilled personnel. These limitations have spurred development of sensors incorporating nanostructured materials that can aid in specific and accurate quantification of HbA1c. Various chemical and biological sensing elements with and without nanoparticle interfaces have been explored for HbA1c detection. Attempts are underway to improve the detection speed, increase accuracy, and reduce sample volumes and detection costs through different combinations of nanomaterials, interfaces, capture elements, and measurement techniques. This review elaborates on the recent advances in the realm of electrochemical detection for HbA1c detection. It also discusses the emerging trends and challenges in the fabrication of effective, accurate, and cost-effective point-of-care (PoC) devices for HbA1c and the potential way forward.

show abstract

“…Eggshell membranes (ESMs), a natural porous fiber, are used as a low-cost protein-immobilization platform. By using 3-APBA-modified ESMs biosensor, the HbA1c can be measured in a clinically relevant range (2.3~14%) with a detection limit of 0.19% [ 31 ]. The EIS biosensors exhibit good reproducibility, precision and selectivity.…”

Section: Hba1c Biosensorsmentioning

confidence: 99%

Current Status of HbA1c Biosensors

Hua

2017

Sensors

View full text Add to dashboard Cite

Glycated hemoglobin (HbA1c) is formed via non-enzymatic glycosylation reactions at the α–amino group of βVal1 residues in the tetrameric Hb, and it can reflect the ambient glycemic level over the past two to three months. A variety of HbA1c detection methods, including chromatography, immunoassay, enzymatic measurement, electrochemical sensor and capillary electrophoresis have been developed and used in research laboratories and in clinics as well. In this review, we summarize the current status of HbA1c biosensors based on the recognition of the sugar moiety on the protein and also their applications in the whole blood sample measurements.

show abstract

Selective label-free electrochemical impedance measurement of glycated haemoglobin on 3-aminophenylboronic acid-modified eggshell membranes

Cited by 11 publications

References 53 publications

Magnetite-Quantum Dot Immunoarray for Plasmon-Coupled-Fluorescence Imaging of Blood Insulin and Glycated Hemoglobin

Magnetite-Quantum Dot Immunoarray for Plasmon-Coupled-Fluorescence Imaging of Blood Insulin and Glycated Hemoglobin

Trends in Quantification of HbA1c Using Electrochemical and Point-of-Care Analyzers

Current Status of HbA1c Biosensors

Contact Info

Product

Resources

About