Selective isolation of hemoglobin by use of imidazolium-modified polystyrene as extractant

Abstract

Ionic liquids have attracted much attention in the analysis of a variety of species. The functional groups in ionic liquids can result in highly efficient separation and enrichment and, because of their typical lack of volatility, they are environmentally benign. We grafted imidazole cations onto the surface of chloromethyl polystyrene, denoted PS-CH2-[MIM](+)Cl(-), and this modified polymer was used to selectively extract the protein hemoglobin (Hb). The prepared extractant PS-CH2-[MIM](+)Cl(-), containing 2 mmol immobilized imidazole groups per gram polymer, was characterized by FT-IR, surface charge analysis, and elemental analysis. The adsorption efficiency was 91%. The adsorption capacity of the PS-CH2-[MIM](+)Cl(-) for Hb was 23.6 μg mg(-1), and 80% of the retained Hb could be readily recovered by use of 0.5% (m/v) aqueous sodium dodecyl sulfate (SDS) solution as eluate. The activity of the eluted Hb was approximately 90%. The prepared imidazole-containing solid phase polymer was used for direct adsorption of Hb without use of any other solid matrix as support of the ionic liquid. The material was used in practice to isolate Hb from human whole blood.

Ionic-Liquid-Mediated Extraction and Separation Processes for Bioactive Compounds: Past, Present, and Future Trends

“…286 The selective isolation of Hb was further studied using imidazolium-modified polystyrene materials. 287 In this work, imidazolium cations were grafted onto the surface of a chloromethyl polystyrene, forming PS-CH 2 -[C 1 im] + Cl – . 287 This process was different from that previously reported by Shu et al, 286 since it was not necessary to introduce any solid substrate (e.g., silica beads).…”

“…287 In this work, imidazolium cations were grafted onto the surface of a chloromethyl polystyrene, forming PS-CH 2 -[C 1 im] + Cl – . 287 This process was different from that previously reported by Shu et al, 286 since it was not necessary to introduce any solid substrate (e.g., silica beads). This is a result of the cross-linked rigid polymer that can act as a support.…”

Advances Achieved by Ionic-Liquid-Based Materials as Alternative Supports and Purification Platforms for Proteins and Enzymes

“…It was also pointed out by the authors that the prepared hybrid material exhibited potential properties crucial for its separation performance: for instance, an outstanding selectivity to adsorb basic proteins through an effective suppression of the adsorption of non-specific proteins by the pure PVC material, as well as its enhanced biocompatibility that avoids protein denaturation phenomenon during adsorption. In a different work, the selective isolation of Hb was assessed using imidazolium-modified polystyrene (PS) materials [87]. Imidazolium cations ([mim] + ) were attached onto the chloromethyl polystyrene (PS-CH 2 -Cl) surface, forming PS-CH 2 -[mim]Cl, which is a crosslinked rigid polymer that can behave as a support.…”

Preliminary assessment of the modification of polystyrene‐divinylbenzene resin with lipid‐tethered ligands for selective separations

“…More recently, Zhao et al. modified chloromethyl PS‐DVB resin with the imidazolium functionality, which is normally employed for immobilized metal affinity chromatography (IMAC) . The use of a simple hydrophobic adsorption mechanism has previously been used to facilitate polyvinyl alcohol cross‐linking on PS‐DVB resin .…”

“…Polystyrene resins are commonly modified with 1-chloromethyl ether using a Friedel-Crafts reaction, to create chloromethyl-derivatized resins for solid-phase peptide synthesis [14]. More recently, Zhao et al modified chloromethyl PS-DVB resin with the imidazolium functionality, which is normally employed for immobilized metal affinity chromatography (IMAC) [15]. The use of a simple hydrophobic adsorption mechanism has previously been used to facilitate polyvinyl alcohol cross-linking on PS-DVB resin [16].…”