Selective isolation of Aspergillus flavus and A. parasitices in field soils in Nansei Shoto in Japan

Suzuki, M.; Narita, N; Kikuchi, Yutaka; Ichinoe, Masakatsu

doi:10.2520/myco1975.1993.23

Cited by 3 publications

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“…Since 1993, aflatoxin B 1 has been detected in muscovado that is produced in the southernmost islands of Japan [5]. Our previous survey revealed that Aspergillus Section Flavi isolates, producing aflatoxins B and G, are predominantly distributed in sugarcane field soil [6]. These fungi were classified as atypical and typical A. parasiticus or A. flavus according to their morphological characteristics [7].…”

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confidence: 99%

High prevalence of B and G aflatoxin-producing fungi in sugarcane field soil in Japan: heteroduplex panel analysis identifies a new genotype within Aspergillus Section Flavi and Aspergillus nomius

Kumeda

Asao

Takahashi

et al. 2003

FEMS Microbiology Ecology

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Heteroduplex panel analysis (HPA) was previously developed for genetic identification of Aspergillus Section Flavi strains, utilizing polymerase chain reaction-amplified fragments of the internal transcribed spacer (ITS) regions of the rRNA gene. Application of HPA to a field study demonstrated that a new type of FP-1 strains belonging to Section Flavi is predominantly distributed throughout sugarcane field soil in the southernmost islands of Japan, and such a trend may also be the case in Vietnam. All of the 71 tested isolates of type FP-1 were able to produce aflatoxins B and G. The morphological observations of the type FP-1 isolates showed that a major part of them had broad interfaces with Aspergillus parasiticus and the remainder with Aspergillus flavus. Phylogenetic analysis based on the ITS sequences indicated that type FP-1 formed an independent clade positioned between A. parasiticus and A. flavus, and was more closely related to the former species. This is also the first report on the distribution of Aspergillus nomius in sugarcane field soil and/or sugarcane stems in Japan and Vietnam.

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Section: Introductionmentioning

confidence: 99%

High prevalence of B and G aflatoxin-producing fungi in sugarcane field soil in Japan: heteroduplex panel analysis identifies a new genotype within Aspergillus Section Flavi and Aspergillus nomius

Kumeda

Asao

Takahashi

et al. 2003

FEMS Microbiology Ecology

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Aspergillus parasiticusCommunities Associated with Sugarcane in the Rio Grande Valley of Texas: Implications of Global Transport and Host Association WithinAspergillusSectionFlavi

Garber¹,

Cotty²

2014

Phytopathology®

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In the Rio Grande Valley of Texas (RGV), values of maize and cottonseed crops are significantly reduced by aflatoxin contamination. Aflatoxin contamination of susceptible crops is the product of communities of aflatoxin producers and the average aflatoxin-producing potentials of these communities influence aflatoxin contamination risk. Cropping pattern influences community composition and, thereby, the epidemiology of aflatoxin contamination. In 2004, Aspergillus parasiticus was isolated from two fields previously cropped to sugarcane but not from 23 fields without recent history of sugarcane cultivation. In 2004 and 2005, A. parasiticus composed 18 to 36% of Aspergillus section Flavi resident in agricultural soils within sugarcane-producing counties. A. parasiticus was not detected in counties that do not produce sugarcane. Aspergillus section Flavi soil communities within sugarcane-producing counties differed significantly dependent on sugarcane cropping history. Fields cropped to sugarcane within the previous 5 years had greater quantities of A. parasiticus (mean = 16 CFU/g) than fields not cropped to sugarcane (mean = 0.1 CFU/g). The percentage of Aspergillus section Flavi composed of A. parasiticus increased to 65% under continuous sugarcane cultivation and remained high the first season of rotation out of sugarcane. Section Flavi communities in fields rotated to non-sugarcane crops for 3 to 5 years were composed of <5% A. parasiticus, and fields with no sugarcane history averaged only 0.2% A. parasiticus. The section Flavi community infecting RGV sugarcane stems ranged from 95% A. parasiticus in billets prepared for commercial planting to 52% A. parasiticus in hand-collected sugarcane stems. Vegetative compatibility assays and multilocus phylogenies verified that aflatoxin contamination of raw sugar was previously attributed to similar A. parasiticus in Japan. Association of closely related A. parasiticus genotypes with sugarcane produced in Japan and RGV, frequent infection of billets by these genotypes, and the ephemeral nature of A. parasiticus in RGV soils suggests global transport with sugarcane planting material.

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A simple genetic method for identification of mycotoxigenic fungi-Development of heteroduplex panel analysis and its field application-

久米田¹

2008

Mycotoxins

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Classification and identification of Aspergillus section Flavi have still relied mainly upon the morphological characters used as taxonomic criteria. It is, however, difficult to differentiate the species because of the wide intra-and interspecific diversity of morphological characteristics 1). Recently, attempts have been made to classify Aspergillus section Flavi by molecular genetic techniques based on restriction fragment length polymorphism (RFLP) with 2) or without 3) using Southern hybridization, random amplified polymorphic DNA (RAPD) 4) , and sequence divergence of aflR genes involved in the aflatoxin pathway 5). The above methods have all provided useful

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Selective isolation of Aspergillus flavus and A. parasitices in field soils in Nansei Shoto in Japan

Cited by 3 publications

References 0 publications

High prevalence of B and G aflatoxin-producing fungi in sugarcane field soil in Japan: heteroduplex panel analysis identifies a new genotype within Aspergillus Section Flavi and Aspergillus nomius

High prevalence of B and G aflatoxin-producing fungi in sugarcane field soil in Japan: heteroduplex panel analysis identifies a new genotype within Aspergillus Section Flavi and Aspergillus nomius

Aspergillus parasiticusCommunities Associated with Sugarcane in the Rio Grande Valley of Texas: Implications of Global Transport and Host Association WithinAspergillusSectionFlavi

A simple genetic method for identification of mycotoxigenic fungi-Development of heteroduplex panel analysis and its field application-

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