Selective Isolation of a Eucalyptus spp. Woodchip Bacterial Community and Its Taxonomic and Metabolic Profiling

Nnadozie, Chika Felicitas; Lin, Johnson; Govinden, Roshini

doi:10.1007/s12155-017-9816-9

Cited by 4 publications

(1 citation statement)

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“…If proportions are kept regarding what we observe in the other comparisons, a higher number of DPGs between Nasutitermes and each of the soil feeders, it is logical to think that we did not have enough number of "events" for statistical differences to appear. We found interesting differences, as three of the most differentially present genes in the wood-feeder nest belong to the glycosyl transferase family (GT 2, 4, and 51), previously reported in woodchip communities but not related to the cellulose hydrolysis (Nnadozie et al, 2017).…”

Section: Shotgun Metagenomic Sequencingmentioning

confidence: 62%

The tropical cookbook: Termite diet and phylogenetics—Over geographical origin—Drive the microbiome and functional genetic structure of nests

Plaza

Hradecký

2023

Front. Microbiol.

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Termites are key decomposers of dead plant material involved in the organic matter recycling process in warm terrestrial ecosystems. Due to their prominent role as urban pests of timber, research efforts have been directed toward biocontrol strategies aimed to use pathogens in their nest. However, one of the most fascinating aspects of termites is their defense strategies that prevent the growth of detrimental microbiological strains in their nests. One of the controlling factors is the nest allied microbiome. Understanding how allied microbial strains protect termites from pathogen load could provide us with an enhanced repertoire for fighting antimicrobial-resistant strains or mining for genes for bioremediation purposes. However, a necessary first step is to characterize these microbial communities. To gain a deeper understanding of the termite nest microbiome, we used a multi-omics approach for dissecting the nest microbiome in a wide range of termite species. These cover several feeding habits and three geographical locations on two tropical sides of the Atlantic Ocean known to host hyper-diverse communities. Our experimental approach included untargeted volatile metabolomics, targeted evaluation of volatile naphthalene, a taxonomical profile for bacteria and fungi through amplicon sequencing, and further diving into the genetic repertoire through a metagenomic sequencing approach. Naphthalene was present in species belonging to the genera Nasutitermes and Cubitermes. We investigated the apparent differences in terms of bacterial community structure and discovered that feeding habits and phylogenetic relatedness had a greater influence than geographical location. The phylogenetic relatedness among nests' hosts influences primarily bacterial communities, while diet influences fungi. Finally, our metagenomic analysis revealed that the gene content provided both soil-feeding genera with similar functional profiles, while the wood-feeding genus showed a different one. Our results indicate that the nest functional profile is largely influenced by diet and phylogenetic relatedness, irrespective of geographical location.

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Section: Shotgun Metagenomic Sequencingmentioning

confidence: 62%