Characterization of cellulolytic microbial consortium enriched on Napier grass using metagenomic approaches

Kanokratana, Pattanop; Wongwilaiwalin, Sarunyou; Mhuantong, Wuttichai; Tangphatsornruang, Sithichoke; Eurwilaichitr, Lily; Champreda, Verawat

doi:10.1016/j.jbiosc.2017.10.014

Cited by 33 publications

(31 citation statements)

References 57 publications

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“…Our present study firstly demonstrated that Stenotrophomonas was positively correlated with BDCA2 + pDCs. Comamonas was a cellulolytic microbe, which could impact the host metabolism in cancer patients and are associated with inflammation (95). Recently, Garcia-Gonzalez et al has demonstrated that Comamonas could decrease 5-fluoro-2'-deoxyuridine efficacy, but increase the efficacy of the topoisomerase I inhibitor camptothecin (96).…”

Section: Discussionmentioning

confidence: 99%

Regulatory T Cells and Plasmacytoid Dendritic Cells Within the Tumor Microenvironment in Gastric Cancer Are Correlated With Gastric Microbiota Dysbiosis: A Preliminary Study

et al. 2019

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Substantial evidence indicates that gastric microbiota dysbiosis, immune system dysfunction especially immune escape are critical for gastric cancer (GC) occurrence and progression. As two important elements of tumor microenvironment (TME), the relationship between gastric microbiota and tumor-immune microenvironment is still unclear. Our present study aimed to explore the correlation between gastric mucosal microbiota in different microhabitats and its corresponding gastric immunosuppressive cells such as regulatory T cells (Tregs) and plasmacytoid dendritic cells (pDCs) in the TME. A cohort of 64 GC patients without preoperative chemotherapy was enrolled retrospectively, and 60 normal, 61 peritumoral and 59 tumoral tissues were obtained for gastric mucosal microbiota analysis and immunohistochemistry analysis. From different microhabitats, BDCA2+pDCs and Foxp3+Tregs were observed positively correlated, and increased in tumoral and peritumoral tissues compared to normal ones. The diversity, composition and function of gastric mucosal microbiota also changed more significantly in tumoral tissues than those in normal and peritumoral ones. With pearson's correlation analysis, we found that several non-abundant genera such as Stenotrophomonas and Selenomonas were positively correlated with BDCA2+pDCs and Foxp3+Tregs, respectively, while Comamonas and Gaiella were negatively correlated with BDCA2+pDCs and Foxp3+ Tregs, respectively. The increased BDCA2+pDCs and Foxp3+Tregs might be modulated by gastric mucosal microbiota, both participated in the immunosuppression microenvironment of GC, which might provide evidence to establish new strategies in antitumor therapy targeting on gastric microbiota.

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Section: Discussionmentioning

confidence: 99%

Regulatory T Cells and Plasmacytoid Dendritic Cells Within the Tumor Microenvironment in Gastric Cancer Are Correlated With Gastric Microbiota Dysbiosis: A Preliminary Study

et al. 2019

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“…In addition, when there were 2 or more metastatic lymph nodes, the levels of Tepidimonas , Enhydrobacter , Turicibacter , and Wautersiella were lower in normal tissues and the levels of Comamonas and Turicibacter were higher in tumor tissues ( Figure 2 F). Comamonas , one of the microbiota taxa with a higher abundance in tumor tissues, is a cellulolytic microbe that can affect the metabolism of cancer patients [ 28 , 29 ], which can be used to construct a predictive model of biliary tract cancer [ 30 ]. On the other hand, Turicibacter is part of the core microbiota of the rat digestive tract [ 31 ] and has been reported to be significantly higher in a dog model of multicentric lymphoma [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Diversity in the Extracellular Vesicle-Derived Microbiome of Tissues According to Tumor Progression in Pancreatic Cancer

Jeong

Kim

et al. 2020

Cancers

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This study was conducted to identify the composition and diversity of the microbiome in tissues of pancreatic cancer and to determine its role. First, extracellular vesicles (EVs) were obtained from the paired tumor and normal tissues, and 16s rRNA gene sequencing was performed. We identified the microbiomes, compared the diversity between groups, and found that Tepidimonas was more abundant in tumors. Second, larger tumors resulted in lower levels of Leuconostoc and Sutterella, and increased lymph node metastasis resulted in higher levels of Comamonas and Turicibacter in tumor tissues. Moreover, in the case of tumor recurrence, the levels of Streptococcus and Akkermansia were decreased in tumor tissues. Finally, with the supernatant of Tepidimonasfonticaldi, proliferation and migration of cells increased, and epithelial-mesenchymal transition and the Tricarboxylic Acid (TCA) cycle-related metabolites were enhanced. The composition and diversity of EV-derived microbiomes are important for providing novel insights into theragnostic approaches in pancreatic cancer.

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“…Cellulolytic enzymes are synthesized by aerobic and anaerobic bacteria, actinomycetes, fungi, and certain protozoa. The bacteria with such activity, belong to Clostridium, Aeromonas, Enterobacter, Actino myces, Bacillus, Bacteroides, Butyrvibrio, Ruminococcs, Methanobrevibacter, Sedimentibacter, Comamonas, and other genera (Gupta et al 2012;Li et al 2016;Kanokratana et al 2018). The strains with high cellulolytic activity isolated in our work were identified as Bacillus spp.…”

Section: Resultsmentioning

confidence: 54%

The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota Isolated from Natural Environment

Wita

Białas

Wilk³

et al. 2019

Polish Journal of Microbiology

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Bacteria from the genus Bacillus are a rich source of commercial enzymes, including amylases, proteases, cellulases, glucose isomerase, and pullulanase. Cellulases account for 15% of the global market of industrial enzymes; thus, new microorganisms producing cellulases in a higher concentration and new ingredients, which can enhance the level of enzyme synthesis, are still needed. Many of cellulose-degrading microorganisms have been isolated so far and characterized in various regions of the world. In this study, we were looking for the bacteria isolated from the natural environment with the high cellulolytic potential, which could be used as components of a biopreparation to accelerate decomposition of postharvest leftovers in agriculture. The 214 bacterial strains were isolated from environmental samples rich in cellulose and their ability to synthesize cellulases were examined using the diffusion method. Six strains, which have the highest diameter of clearing zone both for biomass and supernatant, were selected for identification. Optimization of biosynthesis of the cellulosedegrading enzymes indicated that optimal temperature of this process fluctuated in the range of 21-42°C (depending on the strain and carbon source). The highest cellulolytic activity was observed for the isolates designed as 4/7 (identified as Bacillus subtilis) and 4/18 (identified as Bacillus licheniformis) in a temperature of 32°C. With the use of a desirability function methodology, the optimal medium composition to achieve a simple, cost-efficient process of cellulases production was developed for both strains. These experiments show that microorganisms isolated from natural environmental samples have unique properties and potential for commercial applications (e.g. for biopreparations production).

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Characterization of cellulolytic microbial consortium enriched on Napier grass using metagenomic approaches

Cited by 33 publications

References 57 publications

Regulatory T Cells and Plasmacytoid Dendritic Cells Within the Tumor Microenvironment in Gastric Cancer Are Correlated With Gastric Microbiota Dysbiosis: A Preliminary Study

Regulatory T Cells and Plasmacytoid Dendritic Cells Within the Tumor Microenvironment in Gastric Cancer Are Correlated With Gastric Microbiota Dysbiosis: A Preliminary Study

Diversity in the Extracellular Vesicle-Derived Microbiome of Tissues According to Tumor Progression in Pancreatic Cancer

The Influence of Temperature and Nitrogen Source on Cellulolytic Potential of Microbiota Isolated from Natural Environment

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