Selective inhibition of 5‐lipoxygenase by natural compounds isolated from Chinese plants, <i>Artemisia rubripes</i> Nakai

Koshihara, Yasuko; Neichi, Tomohiro; Murota, Sei‐itsu; Lao, Aina; Fujimoto, Yasuo; Tanaka, Takashi

doi:10.1016/0014-5793(83)80672-3

Cited by 98 publications

(76 citation statements)

References 8 publications

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“…One study was done by Lee et al (2008) and the other by Kim (2005) and associates reported the cytotoxicity of Eupatilin on gastric cancer (Kim et al, 2005b;Lee et al, 2008). Other studies also reported cytotoxic activity of Eupatilin against breast cancer (Kim et al, 2005a), leukemia (Seo and Surh, 2001), mast tumor (Koshihara et al, 1983) and colon cancer cells (Nam et al, 2008;Schulze-Osthoff et al, 1994;Wyllie, 1980;Zhang and Xu, 2000). Our study is the first report of the cytotoxic activity by Eupatilin against melanoma.…”

Section: Cell Growth Inhibition Of A375 Melanoma Cells By Eupatilinsupporting

confidence: 61%

“…(2005a) during their studies on ras transformed breast epithelial cells (MCF-10A-ras). Antitumor potential of Eupatilin is also reported by Seo and Surh (2001) in leukemia HL60 cells, (Koshihara et al, 1983) in mast tumor and (Nam et al, 2008) in colon cancer cells. However, none of the previously published reports have shown an activity of Eupatilin against melanoma.…”

Section: Introductionmentioning

confidence: 78%

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Eupatilin: A flavonoid compound isolated from the artemisia plant, induces apoptosis and G2/M phase cell cycle arrest in human melanoma A375 cells

Shawi#¹

2011

Afr. J. Pharm. Pharmacol.

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Eupatilin is a flavonoid compound isolated from the Artemisia plant. Eupitillin possesses antioxidant as well as potent anticancer properties. In this study, for the first time, we examined the anti-proliferative effects of Eupatilin in human melanoma A375 cells and its ability to induce apoptosis and cell cycle arrest. Eupatilin specifically induced morphological changes in A375 cells and was less toxic to the normal mouse splenocytes. The inhibitory effects of A375 cells were associated with the DNA damage, apoptosis, and cell cycle arrest at G2/M phase in a dose-dependent manner. The apoptotic effects of Eupatilin were further verified by using Annexin V-FITC/propidium iodide staining in flow cytometry. These results suggest that Eupatilin is an effective natural compound that worth further mechanistic and therapeutic studies against human melanoma.

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Section: Cell Growth Inhibition Of A375 Melanoma Cells By Eupatilinsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 78%

Eupatilin: A flavonoid compound isolated from the artemisia plant, induces apoptosis and G2/M phase cell cycle arrest in human melanoma A375 cells

Shawi#¹

2011

Afr. J. Pharm. Pharmacol.

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“…Phenolics have an array of health-promoting benefits; they are of current interest due to their important biological and pharmacological properties, especially the antiinflammatory [30], antioxidant [31], and antimutagenic and anticarcinogenic activities [32,33]. Since they are widespread in plant-based foods, humans consume phenolic acids on a daily basis.…”

Section: Phenolic Acids and Flavonoids In Human Healthmentioning

confidence: 99%

Extraction, separation, and detection methods for phenolic acids and flavonoids

Stalikas¹

2007

J of Separation Science

811

539

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ReviewExtraction, separation, and detection methods for phenolic acids and flavonoidsThe impetus for developing analytical methods for phenolic compounds in natural products has proved to be multifaceted. Hundreds of publications on the analysis of this category of compounds have appeared over the past two decades. Traditional and more advanced techniques have come to prominence for sample preparation, separation, detection, and identification. This review provides an updated and extensive overview of methods and their applications in natural product matrices and samples of biological origin. In addition, it critically appraises recent developments and trends, and provides selected representative bibliographic examples.

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“…In 1981, Bokoch and Reed published the polyphenol nordihydroguaiaretic acid 41 (NDGA) from the Mexican dessert plant Larrea divaricata as the first plant-derived 5-LO inhibitor [65]. Later, Koshihara et al reported about natural compounds isolated from the Chinese plant Artemisia rubris, which were caffeic acid 36, eupatilin and 4-demethyleupatilin that inhibited 5-LO activity in a cell free-assay as well as the formation of LTC 4 and D 4 in ionophore-stimulated mastocytoma cells [89]. Like NDGA 41, caffeic acid 36 is a lipophilic phenolic compound, and eupatilin and 4-demethyleupatilin are flavones, and all of them possess reducing properties, thus, acting as antioxidants.…”

Section: Inhibitors Of 5-lo Product Synthesis From Plant Originmentioning

confidence: 99%

Inhibition of 5-Lipoxygenase Product Synthesis by Natural Compounds of Plant Origin

Werz¹

2007

Planta Med

149

135

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The biosynthesis of leukotrienes (LTs) is initiated by the transformation of free arachidonic acid to LTA 4 by 5-lipoxygenase (5-LO). Subsequent enzymatic conversion of LTA 4 yields LTB 4 and the cysteinyl-LTs C 4 , D 4 and E 4 . LTs have prominent functions in pathophysiology and are connected to numerous disorders including bronchial asthma, allergic rhinitis, inflammatory bowel and skin diseases, rheumatoid arthritis, cancer, osteoporosis and cardiovascular diseases. Pharmacological and genetic interruption of the 5-LO pathway or blockade of LT receptors, serving as means for intervention with LTs, may be of therapeutic value for certain related disorders. Natural or plant-derived substances were among the first 5-LO inhibitors identified in the early 1980 s. To date, a huge number of diverse plant-derived compounds have been reported to interfere with 5-LO product synthesis. However, many investigations have addressed the efficacy of a given compound solely in cellular test systems and analysis of direct interference with 5-LO has been neglected. In the first part of this review, the biology and molecular pharmacology of the 5-LO pathway is summarized in order to understand its overall regulation and complexity as well as to comprehend the possible points of attack of compounds that eventually lead to inhibition of 5-LO product formation in intact cells. In the second part, natural compounds that interfere with 5-LO product formation are compiled and grouped into structural classes, and the underlying molecular mechanisms and structureactivity relationships are discussed.

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Selective inhibition of 5‐lipoxygenase by natural compounds isolated from Chinese plants, Artemisia rubripes Nakai

Cited by 98 publications

References 8 publications

Eupatilin: A flavonoid compound isolated from the artemisia plant, induces apoptosis and G2/M phase cell cycle arrest in human melanoma A375 cells

Eupatilin: A flavonoid compound isolated from the artemisia plant, induces apoptosis and G2/M phase cell cycle arrest in human melanoma A375 cells

Extraction, separation, and detection methods for phenolic acids and flavonoids

Inhibition of 5-Lipoxygenase Product Synthesis by Natural Compounds of Plant Origin

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