Selective growth of epithelial basal cells from human prostate in a three‐dimensional organ culture

Abstract

These systems allow the study of proliferation and differentiation of basal cells within their natural microenvironment as well as prostate pathobiology.

Selective growth and expansion of human corneal epithelial basal stem cells in a three-dimensional-organ culture

“…Immune‐staining showed that a great number of them expressed constitutively both p63 (>85% positive)(A) and CK‐19 (over 90% positive) (B) markers and all cells were constantly CK‐3 negative (C), clearly representing an enriched and rather homogeneous source of epithelial stem/progenitor corneal basal epithelial cells (basal cell metaplasia), consistent with their limbal origin (Joseph et al, 2004). These results are in line with a similar behavior that we have previously reported when trying to isolate selectively basal epithelial cells from human skin (Papini et al, 2003) or prostatic epithelium (Papini et al, 2004) in a similar type of 3D‐organ culture.…”

“…No. NDC 009‐031503) (20 × 60 × 7 mm size) were moisturized with Epilfe 10 and pressed to release air bubbles just before use, as described (Hanto et al, 1982; Chishima et al, 1997; Chen et al, 2003; Papini et al, 2003, 2004). Each sponge was cut into three pieces (20 × 20 × 7 mm size), and each piece was transferred to a well of a six‐well tissue culture plate (Falcon Plastics Inc.; London Ontario, Canada; Cat.…”

Prostate epithelial cell differentiation and its relevance to the understanding of prostate cancer therapies

“…The androgen receptor is also present [6,54], mediating the production and secretion of PSA and PAP [55]. ET-1 (endothelin-1) is present in normal luminal cells, and low levels of PSMA (prostate surface membrane antigen) have also been detected [56][57][58]. As a result of the differentiation process, luminal cells do not express detectable levels of Bcl-2 [41,42].…”

Plant Science and Human Nutrition: Challenges in Assessing Health-Promoting Properties of Phytochemicals

“…Availability of tissue is also an issue that prevents their use in medium-to highthroughput analyses of biological efficacy. Nevertheless, they can be valuable in understanding biochemical processes in response to plant secondary metabolites (Mehta and Lansky, 2004;Papini et al, 2004;Moronvalle-Halley et al, 2005;Konsue and Ioannides, 2010).…”