Selective fluorescence detection of cysteine and N-terminal cysteine peptide residues

Abstract

A new fluorogenic fluorescein derivative containing an α,β-unsaturated aldehyde moiety produces a selective fluorescent signal enhancement in the presence of cysteine or peptides containing N-terminal cysteine residues. The mechanism is based on synergistic covalent and supramolecular interactions.

Photoelectrochemistry of Free‐Base‐Porphyrin‐Functionalized Zinc Oxide Nanoparticles and Their Applications in Biosensing

“…[44] The response could reach a steady signal within only 3 s. The time for detection of cysteine was much shorter than that of 20 min for the fluorescence spectra method. [38] Therefore, the photoelectrochemical biosensor prepared with TCPP-ZnO showed a good performance for the determination of cysteine due to the effective charge separation and interfacial photoinduced hole transfer at the surface of TCPP-ZnO-modified film.…”

“…Since the normal level of cysteine in human plasma is in the range of 240-360 mmol L À1 , [31,58,59] the present approach had enough sensitivity for the detection of cysteine in biological samples. The detection limit of 0.2 mmol L À1 was much lower than 39 mmol L À1 by fluorescence spectra methods, [38] and 0.8 mmol L À1 by UV absorption spectra. [44] The response could reach a steady signal within only 3 s. The time for detection of cysteine was much shorter than that of 20 min for the fluorescence spectra method.…”

A New Turn on Fluorescent Probe for Selective Detection of Cysteine/Homocysteine

“…Significant efforts have been devoted to construction of fluorescent probes for biothiols [7]. Many fluorescent probes involved in different detecting mechanisms have been developed for thiols detecting, such as Michael addition reaction [8][9][10][11][12][13][14][15][16], cyclization reaction based on aminothiols and aldehyde [17][18][19][20][21], cleavage reaction induced by thiols [22][23][24][25][26][27][28][29][30], and ligand displacement of metal complexes by thiols [31][32][33].…”

Carboxymethylagarose-AuNPs generated through green route for selective detection of Hg2+ in aqueous medium with a blue shift

“…Thus it is very important to develop an extremely high sensitivity, cost-effective and bio-based Hg 2+ sensor that can provide real-time determination of Hg 2+ levels in the environment, water, and food (Darbha, Ray, & Ray, 2007). A great effort has been exerted to detect Hg 2+ using various detection techniques, including optical spectroscopy (Jiang et al, 2012;Lim, Escobedo, Lowry, Xu, & Strongin, 2010;Rusin et al, 2003;Wang et al, 2005;Wang, Heon Lee, & Lu, 2008), electrochemical methods (Liu, Nie, Jiang, Shen, & Yu, 2009;Spãtaru, Sarada, Popa, Tryk, & Fujishima, 2001), high-performance liquid chromatography (Chen, Chen, Jin, & Wei, 2009;Lu, Zu, & Yam, 2007), inductively coupled plasma mass spectrometry, and so forth (Chen et al, 2009;Li et al, 2006). However, most of these techniques require expensive instrumentation and complicated sample preparation in certain cases, which make them inappropriate for point of use applications.…”