Selective expression of aquaporin 1, 4 and 5 in the rat middle ear

Minami, Shin-ichi; Kobayashi, Hideyuki; Yamashita, Atsushi; Yanagita, Toshihiko; Uezono, Yasuhito; Yokoo, Hiroki; Shiraishi, Seiji; Saitoh, Tomokazu; Yujiro, Asada; Komune, Shizuo; Wada, Akihiko

doi:10.1016/s0378-5955(01)00284-2

Cited by 13 publications

(13 citation statements)

References 12 publications

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“…The inner ear contains several types of supporting cells. Some of these cell types contain AQP4, while others do not (66,103,105,108,181). As in the retina, the distribution of AQP4 is consistent with an indirect role in K ϩ clearance.…”

Section: B Inner Earsupporting

confidence: 54%

Physiological Roles of Aquaporin-4 in Brain

Nagelhus

Ottersen

2013

Physiological Reviews

577

520

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(AQP4) is one of the most abundant molecules in the brain and is particularly prevalent in astrocytic membranes at the blood-brain and brain-liquor interfaces. While AQP4 has been implicated in a number of pathophysiological processes, its role in brain physiology has remained elusive. Only recently has evidence accumulated to suggest that AQP4 is involved in such diverse functions as regulation of extracellular space volume, potassium buffering, cerebrospinal fluid circulation, interstitial fluid resorption, waste clearance, neuroinflammation, osmosensation, cell migration, and Ca 2ϩ signaling. AQP4 is also required for normal function of the retina, inner ear, and olfactory system. A review will be provided of the physiological roles of AQP4 in brain and of the growing list of data that emphasize the polarized nature of astrocytes.

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Section: B Inner Earsupporting

confidence: 54%

Physiological Roles of Aquaporin-4 in Brain

Nagelhus

Ottersen

2013

Physiological Reviews

577

520

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“…These correlative findings support the notion that AQP1 is involved in maintaining the ion gradient in the subepithelial milieu of the middle ear in guinea pigs. AQP1 is also localized on capillary endothelial cells and fibroblasts in lamina propria mucosae in rats [37] . To our knowledge, the present study is the first demonstration of the expression and cellular distribution of AQP1 in the middle ear in guinea pigs.…”

Section: Discussionmentioning

confidence: 95%

Expression Pattern of Aquaporin 1 in the Middle Ear of the Guinea Pig with Secretory Otitis Media

Zhang

Liu²,

Gao³

et al. 2008

ORL

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Objective: To explore the pathological change of water homeostasis in the secretory otitis media (SOM) middle ear, we observed the expression and regulation of aquaporin 1 (AQP1) in the SOM middle ear cavity. Methods: Reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting and immunohistochemical staining were used to detect AQP1 in the bullae of SOM models and normal animals. The expression patterns of AQP1 in the SOM group were compared with those in the normal animal group. Results: RT-PCR and immunoblot analyses revealed that mRNAs encoding AQP1 were expressed in the middle ear membrane of the guinea pigs of both groups; AQP1 was also detected as 28-kDa proteins in both groups. Immunohistochemical analysis showed that AQP1 was localized on capillary endothelial cells and fibroblasts in the lamina propria mucosae as well as on flat and cubical epithelial cells. Quantitative analysis of RT-PCR and Western blotting revealed that AQP1 expression was higher in the SOM group than in the control group. The cellular expression of AQP1 was somewhat altered in the SOM middle ear. Conclusions: Our study suggests that AQP1 in the middle ear cavity may play a vital role in the accumulation of the effusion. It might work on the vessel-caused hydrops in the middle ear cavity.

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“…The distribution of AQP1 in fibroblasts is often observed in fetal and adult stromal tissues (Altuntas et al 2006;Huysseune et al 2009;Minami et al 2001). Roles of AQP1 in cell migration and proliferation have been suggested (Hoque et al 2006;Monzani et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Cellular and subcellular localization of aquaporins 1, 3, 8, and 9 in amniotic membranes during pregnancy in mice

Kobayashi

Yasui

2010

Cell Tissue Res

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The amniotic membrane encloses the amniotic fluid and plays roles in the regulation of amniotic fluid flux through the intramembranous pathway during pregnancy. Aquaporins (AQPs) 1, 3, 8, and 9 are expressed in amniotic membranes. AQPs are water channel proteins that facilitate the rapid flux of water or small molecules across the plasma membrane. Recently, additional roles of AQPs in facilitating cell migration, proliferation, and apoptosis have been suggested, with AQPs being distributed in the appropriate subcellular regions for their functions. The cellular and subcellular distributions of AQPs in the amniotic membrane however remain unclear. We have examined the cellular and subcellular localization of AQPs in amniotic membranes during pregnancy in mice. After embryonic day 12 (E12), AQP1 was distributed in the plasma membrane of finely branched cell processes in the amniotic fibroblasts. AQP3 was present in both epithelial cells and fibroblasts between E10 and E12. The distribution of AQP3 in the epithelial cells dynamically changed as follows: at E14 in the lateral membrane and apical junction; at E16 in the lateral membrane alone; at E17 in the lateral membrane and cytoplasm. AQP8 was expressed in the epithelial cells and complementarily localized in the apical junction and the lateral membrane. AQP9 was detected only in the apoptotic cells of the epithelium. These cellular and subcellular localizations of amniotic AQPs indicate that each AQP plays distinct functional roles, such as in water and urea transport, cell migration, cell proliferation, and apoptosis, for amniotic fluid homeostasis or tissue remodeling of amniotic membranes.

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Selective expression of aquaporin 1, 4 and 5 in the rat middle ear

Cited by 13 publications

References 12 publications

Physiological Roles of Aquaporin-4 in Brain

Physiological Roles of Aquaporin-4 in Brain

Expression Pattern of Aquaporin 1 in the Middle Ear of the Guinea Pig with Secretory Otitis Media

Cellular and subcellular localization of aquaporins 1, 3, 8, and 9 in amniotic membranes during pregnancy in mice

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