Selective determination of cholesterol based on cholesterol oxidase-alkaline phosphatase bienzyme electrode

Zhang, Hongfang; Liu, Ruixiao; Zheng, Jianbin

doi:10.1039/c2an36075f

Cited by 20 publications

(8 citation statements)

References 48 publications

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“…[110] When ALP is placed in a solution containing disodium phenyl phosphate, it produces phenol, which is electroactive. For the dual enzyme electrode, hydrogen peroxide produced by ChOx in the presence of cholesterol, decreased the sensitivity of the electrode for phenol, and produced a lower electrode response with DPV.…”

Section: 0 Enzyme Substratesmentioning

confidence: 99%

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Yang

Denno

Pyakurel

et al. 2015

Analytica Chimica Acta

462

263

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Carbon nanomaterials are advantageous for electrochemical sensors because they increase the electroactive surface area, enhance electron transfer, and promote adsorption of molecules. Carbon nanotubes (CNTs) have been incorporated into electrochemical sensors for biomolecules and strategies have included the traditional dip coating and drop casting methods, direct growth of CNTs on electrodes and the use of CNT fibers and yarns made exclusively of CNTs. Recent research has also focused on utilizing many new types of carbon nanomaterials beyond CNTs. Forms of graphene are now increasingly popular for sensors including reduced graphene oxide, carbon nanohorns, graphene nanofoams, graphene nanorods, and graphene nanoflowers. In this review, we compare different carbon nanomaterial strategies for creating electrochemical sensors for biomolecules. Analytes covered include neurotransmitters and neurochemicals, such as dopamine, ascorbic acid, and serotonin; hydrogen peroxide; proteins, such as biomarkers; and DNA. The review also addresses enzyme-based electrodes that are used to detect non-electroactive species such as glucose, alcohols, and proteins. Finally, we analyze some of the future directions for the field, pointing out gaps in fundamental understanding of electron transfer to carbon nanomaterials and the need for more practical implementation of sensors.

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Section: 0 Enzyme Substratesmentioning

confidence: 99%

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Yang

Denno

Pyakurel

et al. 2015

Analytica Chimica Acta

462

263

View full text Add to dashboard Cite

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“…It also shows high affinity for proteins and availability of reactive functional groups for chemical modifications which make it a perfect enzyme immobilization support [33]. Transducers have been fabricated using nano ZnO film on chitosan [34], Pt-Pd NPs-CS-GS nanocomposite [35], CNT-CS/GCE [36]. Along with conducting polymers non conducting matrices are suitable for enzyme immobilization.…”

Section: Organic and Inorganic Matrices Based Biosensorsmentioning

confidence: 99%

“…Phenol is produced by the action of ALP. Then oxidation of phenol is interpreted for cholesterol determination [36].…”

Section: Nano Structures Based Biosensorsmentioning

confidence: 99%

Recent approaches to ameliorate selectivity and sensitivity of enzyme based cholesterol biosensors: a review

Gahlaut

Hooda

Dhull

2017

Artificial Cells, Nanomedicine, and Biotechnology

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The healthcare area is often reluctant to execute new technology unless they are proven to be safe, constructive and secure. Eventually, an aspiration stands for providing point-of-care testing service to allow a better estimation of the biochemical levels of a patient that entails an insistent remedial action. With increasing mortality rate due to cardiovascular diseases (CVDs) in present scenario, it has become the need of hour to develop more advance methods for their diagnosis, so that it can be determined at sensitive levels and can be prevented from being fatal. Elevated level of cholesterol in blood stream is one of the utmost risk factors which lead to CVDs. Discernible from the vast research in this field, worth of cholesterol biosensors is already recognized and flourished in the clinical analysis of brain and cardiac vascular diseases. It necessitates unremitting progress in the development of biosensing technology towards fabrication, miniaturization and multiplexing ability of cholesterol quantification devices so that they can endow with lab-on-chip-analysis systems to the medical field. Different strategies have been meticulously explored for the engineering of cholesterol biosensors utilizing nanocomposites, conducting polymers, nanotubes and nanoparticles. Foremost, this article reviews the contemporary evolution in cholesterol biosensors, which encompass various strategies for immobilization of enzymes and roles of various matrices and artificial mediators used for the biosensor fabrication. Still there remains an enormous challenge to congregate the demands of performance and yield in a cost effective manner for its application in successful treatments of CVDs.

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“…Multi-target systems can be developed by co-immobilization ( Orrego et al, 2020 ) and have aroused interest due to their different applications, which include screening of ligands ( Chen et al, 2017 ; Wu et al, 2020 ), syntheses of products ( Betancor and Luckarift, 2010 ), design of biocatalyst cascades ( Kang et al, 2014 ), and construction of biosensors to detect diseases ( Zhang et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Co-Immobilized Capillary Enzyme Reactor Based on Beta-Secretase1 and Acetylcholinesterase: A Model for Dual-Ligand Screening

2021

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We have developed a dual enzymatic system assay involving liquid chromatography-mass spectrometry (LC–MS) to screen AChE and BACE1 ligands. A fused silica capillary (30 cm × 0.1 mm i.d. × 0.362 mm e.d.) was used as solid support. The co-immobilization procedure encompassed two steps and random immobilization. The resulting huAChE+BACE1-ICER/MS was characterized by using acetylcholine (ACh) and JMV2236 as substrates. The best conditions for the dual enzymatic system assay were evaluated and compared to the conditions of the individual enzymatic system assays. Analysis was performed in series for each enzyme. The kinetic parameters (KMapp) and inhibition assays were evaluated. To validate the system, galantamine and a β-secretase inhibitor were employed as standard inhibitors, which confirmed that the developed screening assay was able to identify reference ligands and to provide quantitative parameters. The combination of these two enzymes in a single on-line system allowed possible multi-target inhibitors to be screened and identified. The innovative huAChE+BACE1-ICER/MS dual enzymatic system reported herein proved to be a reliable tool to identify and to characterize hit ligands for AChE and BACE1 in an enzymatic competitive environment. This innovative system assay involved lower costs; measured the product from enzymatic hydrolysis directly by MS; enabled immediate recovery of the enzymatic activity; showed specificity, selectivity, and sensitivity; and mimicked the cellular process.

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Selective determination of cholesterol based on cholesterol oxidase-alkaline phosphatase bienzyme electrode

Cited by 20 publications

References 48 publications

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Recent approaches to ameliorate selectivity and sensitivity of enzyme based cholesterol biosensors: a review

Co-Immobilized Capillary Enzyme Reactor Based on Beta-Secretase1 and Acetylcholinesterase: A Model for Dual-Ligand Screening

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