Selective detection of 1000 B. anthracis spores within 15 minutes using a peptide functionalized SERS assay

Farquharson, Stuart; Shende, Chetan; Smith, Wayne; Huang, Hermes; Inscore, Frank; Sengupta, Atanu; Sperry, J. F.; Sickler, Todd; Prugh, Amber; Guicheteau, Jason A.

doi:10.1039/c4an01163e

Cited by 19 publications

(12 citation statements)

References 45 publications

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“…Furthermore, DPA is only a generic marker for the presence of endospores, not solely for B. anthracis endospores. This is the starting point of Farquharson et al ., who combined colloidal SERS with peptide‐functionalized silver particles to detect 10 4 B. anthracis Ames endospores within 10 ml in 15 min . The crucial point in this publication is the peptide‐functionalized silver nanoparticles embedded in a porous glass structure within a capillary, which selectively bound B. anthracis Sterne with a 100‐fold selectivity versus B. cereus , genetically a close neighbor of B. anthracis .…”

Section: Raman Spectroscopy For the Detection Of Bacteria In Environmmentioning

confidence: 99%

The application of Raman spectroscopy for the detection and identification of microorganisms

Stöckel

Kirchhoff

Neugebauer

et al. 2015

J Raman Spectroscopy

202

155

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A fast and reliable detection and identification of microorganisms is crucial in environmental science, for food quality as well as medical diagnosis. In these fields, all types of Raman spectroscopy are gaining more and more importance during the last years. The review provides an extensive overview of recent research, technical expertise, and scientific findings based on Raman spectroscopic detection and identification of microorganisms within the years 2010 and 2015, demonstrating the diverse capability of Raman spectroscopy as a modern analytical tool. Raman spectroscopy distinguishes itself from other currently applied techniques by its easy application at low cost, its high speed of analysis, and its broad information content on both the chemical composition and the structure of biomolecules within the microorganisms. Slight chances in the chemical composition of microorganisms can be monitored by means of Raman spectroscopy and used to differentiate genera, species, or even strains. Detection of pathogens is possible from complex matrices, such as soil, food, and body fluids. Further, spectroscopic studies of host–pathogen interactions are addressed as well as the effect of antibiotics on bacteria. Copyright © 2015 John Wiley & Sons, Ltd.

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Section: Raman Spectroscopy For the Detection Of Bacteria In Environmmentioning

confidence: 99%

The application of Raman spectroscopy for the detection and identification of microorganisms

Stöckel

Kirchhoff

Neugebauer

et al. 2015

J Raman Spectroscopy

202

155

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“…As an alternative, the use of synthetic peptides for this purpose has also been reported. 25,26 More recently, nucleic acid aptamers have been introduced as novel recognition elements. [27][28][29][30][31] Aptamers are short artificial DNA and RNA sequences selectively chosen by their extremely strong binding affinity to their target proteins through a process called Systematic Evolution of Ligands by Exponential Enrichment (SELEX).…”

Section: Preliminary Results and Discussionmentioning

confidence: 99%

Functionalized paper SERS (P-SERS) substrates for selective targeting of analytes in complex samples

Wang¹,

Hoppmann²

2015

SPIE Proceedings

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Surface enhanced Raman spectroscopy (SERS) requires the analyte molecule to be close to the plasmonic surface in order to generate SERS enhancement. This limitation restricts the practical application of SERS to molecules that possess functional groups that interact strongly with gold or silver surfaces. Moreover, the identification of target analytes in a complex sample matrix is made even more difficult when interferents compete with the target for binding to the plasmonic surface, resulting in overlapping spectral signatures. In this work, we report a strategy to functionalize inkjet printed P-SERS substrates by strategically placing supramolecular structures (such as nucleic acid aptamers) onto the gold nanoparticles. This promotes the selective interaction of target molecules with the plasmonic surface, leading to improved sensor performance.

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“…Lu’s group [ 112 ] firstly achieved a LOD of about 4 × 10 −6 M CaDPA based on SERS-active silicon nanowire arrays coated with silver. Farquharson’s group [ 113 ] made use of ATYPLPIR, a peptide having stronger affinity to B. anthracis than other species of Bacillus , to offer the SERS assay with 10 4 spores per mL every 10 min. Cheung et al [ 114 ] proposed a SERS method composed of meso-droplets on superhydrophobic wires and achieved the sensitive detection of DPA, well below the infective dose.…”

Section: Sers Applications In the Detection Of Stress Factorsmentioning

confidence: 99%

Applications of SERS in the Detection of Stress-Related Substances

Chen

Tang

et al. 2018

Nanomaterials

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A wide variety of biotic and abiotic stresses continually attack plants and animals, which adversely affect their growth, development, reproduction, and yield realization. To survive under stress conditions, highly sophisticated and efficient tolerance mechanisms have been evolved to adapt to stresses, which consist of the variation of effector molecules playing vital roles in physiological regulation. The development of a sensitive, facile, and rapid analytical methods for stress factors and effector molecules detection is significant for gaining deeper insight into the tolerance mechanisms. As a nondestructive analysis technique, surface-enhanced Raman spectroscopy (SERS) has unique advantages regarding its biosensing applications. It not only provides specific fingerprint spectra of the target molecules, conformation, and structure, but also has universal capacity for simultaneous detection and imaging of targets owing to the narrow width of the Raman vibrational bands. Herein, recent progress on biotic and abiotic stresses, tolerance mechanisms and effector molecules is summarized. Moreover, the development and promising future trends of SERS detection for stress-related substances combined with nanomaterials as substrates and SERS tags are discussed. This comprehensive and critical review might shed light on a new perspective for SERS applications.

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Selective detection of 1000 B. anthracis spores within 15 minutes using a peptide functionalized SERS assay

Cited by 19 publications

References 45 publications

The application of Raman spectroscopy for the detection and identification of microorganisms

The application of Raman spectroscopy for the detection and identification of microorganisms

Functionalized paper SERS (P-SERS) substrates for selective targeting of analytes in complex samples

Applications of SERS in the Detection of Stress-Related Substances

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