Selective detection and identification of Se containing compounds—review and recent developments

Abstract

The complexity of selenium (Se) chemistry in the environment and in living organisms presents broad analytical challenges. The selective qualitative and quantitative determination of particular species of this element is vital in order to understand selenium's metabolism and significance in biology, toxicology, clinical chemistry and nutrition. This calls for state-of-the-art analytical techniques such as hyphenated methods that are reviewed with particular emphasis on interfaced separation with element-selective detection and identification of the detected selenium compounds. Atomic spectral element specific detection for monitoring chromatographic eluent enabled quantitative determination of selenium species in selenized yeast and qualitative measurement for breath samples. Gas chromatography with atomic emission detection (AED) of ethylated species and fluoroacid ion pair HPLC applied to the analysis of currently produced or archived selenized yeast and Brassica juncea have revealed the presence of a previously unrecognised Se-S amino acid, S-(methylseleno)cysteine.

Fontes orgânicas e inorgânicas de selênio na nutrição de vacas leiteiras: digestão, absorção, metabolismo e exigências

“…As formas orgânicas, por sua vez, estão disponíveis como leveduras enriquecidas, que crescem sobre um substrato contendo pouco enxofre e muito Se. Dessa forma, o Se encontrado é basicamente a SeMet, porém com diversas outras formas ainda desconhecidas (UDEN et al, 2004).…”

Determination of Selenomethionine by High-Performance Liquid Chromatography–Fluorescence Detection Coupled with On-line Oxidation

“…[12][13][14][15][16][17][18] Although ICP-MS enables selective detection of Se, several problems are mentioned by Francesconi et al 2 First, interference by polyatomic species (e.g., 40 For example, when either the standard or the sample compound is in excess, distinguishing two different but chromatographically similar compounds becomes quite difficult. The third point is assignment of the peaks at or near the void volume.…”

Capillary electrophoresis‐inductively coupled plasma‐mass spectrometry: A report on technical principles and problem solutions, potential, and limitations of this technology as well as on examples of application

“…There was 45% comigration with Se-methionine, other Se compounds comigrated with trimethylselenonium, Se-methylselenomethionine and Se-cystineSe-methyl-Se-cysteine. Another short but very valuable overview about selective detection and identification of Se-containing compounds (partly focusing as well on CE-ICP-MS) is provided by Uden et al [58].…”