Selective Consumption of Fish Oil at End of the Day Increases the Physiological Fatty Acid Compositions of Eicosapentaenoic Acid and Docosahexaenoic Acid in Mice

Matsuzaka, Hiroki; Matsuyama, Hiroki; Tanaka, Wataru; Tajiri, Hayato; Sakakibara, Hiroyuki

doi:10.3390/molecules27041271

Cited by 4 publications

(3 citation statements)

References 35 publications

(40 reference statements)

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“…was measured as described previously [18] by an operator who was blinded to the diet that each animal had consumed. Body weight just before dissection was used to determine the body mass index using the following formula (1):…”

Section: Methodsmentioning

confidence: 99%

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Maltose consumption exacerbates high-fat diet-induced overweight and related parameters in mice

Shibazaki¹,

Tanaka²,

Suzuki³

et al. 2022

FFHD

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Background: Maltose is a disaccharide formed from two units of glucose. The amount of maltose in raw sweet potatoes reportedly increased by more than 40-fold after baking. We aimed to investigate the effects of maltose consumption on diet-induced obesity using a mouse model.Methods: Male C57BL/6J mice were divided into three dietary groups: a control diet of American Institute of Nutrition (AIN)-93 (CD), an AIN-93-based 30% high-fat diet (HFD), or an HFD diet containing 7.0% maltose (HFD+Mal). After 13 weeks, body mass index, blood glucose, lipid parameters, including total cholesterol and triglyceride, and hepatic fatty acid content were evaluated. Results: After 6 weeks on the special diet, weight gain was significantly higher in the HFD+Mal group than in the HFD group. The body mass index rose steadily and was significantly higher in the HFD+Mal group (5.03 ± 0.22), compared to the CD (3.32 ± 0.30) and HFD (4.57 ± 0.40) groups at 12 weeks. The relative liver weight per weight was also significantly higher in the HFD+Mal group than in the other two groups. The increases in blood glucose levels were more significant in the HFD+Mal group compared to the HFD group, as were the plasma levels of total cholesterol, high-density lipoprotein (HDL)-cholesterol and non-HDL-cholesterol. In the liver, levels of the following fatty acids increased in both the HFD and HFD+Mal groups relative to those in the CD group: C14:0 (myristic acid), C16:0 (palmitic acid), C18:0 (stearic acid), C18:1 (oleic acid), C18:2 (linoleic acid) and C18:3 (α-linolenic acid). Additionally, the C16:0 content in the HFD+Mal group was significantly higher than that in the HFD group. Conclusion: The results of this study suggest that daily consumption of maltose exacerbated the development of obesity and related parameters, including body mass index and plasma cholesterol levels, under the high-fat diet consumption. It is possible that the consumption of maltose-rich cooked sweet potatoes, as part of an overall HFD, might exacerbate HFD-induced overweight.Keywords: high-fat diet-induced obesity, maltose, mice, roasting, sweet potato

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Section: Methodsmentioning

confidence: 99%

“…Hepatic fatty acid analysis Hepatic fatty acid compositions were analyzed as described in our previous study [18]. Briefly, liver samples (200 mg) were homogenized with 1 mL of 50 mM sodium acetate, and then extracted using a chloroform-methanol mixture followed by Fisher's PLSD post-hoc test.…”

Section: Blood Biochemical Parameters and Hepatic Lipid Analysismentioning

confidence: 99%

Maltose consumption exacerbates high-fat diet-induced overweight and related parameters in mice

Shibazaki¹,

Tanaka²,

Suzuki³

et al. 2022

FFHD

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“…Fatty acid compositions of the BHT-treated blood, serum and oil samples were analyzed using a Fatty Acid Methylation Kit (Nacalai Tesque, Inc., Kyoto, Japan), according to the manufacturer's protocol and the method used previously [21]. Specifically, fatty acids were extracted from 10 µL blood, 25 µL serum and 10 mg oil samples and methylesterified; then, the products were dissolved in 200 µL of hexane.…”

Section: Circulating Fatty Acidsmentioning

confidence: 99%

Daily Consumption of α-Linolenic Acid Increases Conversion Efficiency to Eicosapentaenoic Acid in Mice

Watabe,

Tanaka,

Sakakibara

et al. 2024

Nutrients

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To maintain a beneficial concentration of eicosapentaenoic acid (EPA), the efficient conversion of its precursor, α-linolenic acid (α-LA), is important. Here, we studied the conversion of α-LA to EPA using ICR and C57BL/6 mice. A single dose of perilla oil rich-in α-LA or free α-LA had not been converted to EPA 18 h following administration. The α-LA was absorbed into the circulation, and its concentration peaked 6 h after administration, after which it rapidly decreased. In contrast, EPA administration was followed by an increase in circulating EPA concentration, but this did not decrease between 6 and 18 h, indicating that the clearance of EPA is slower than that of α-LA. After ≥1 week perilla oil intake, the circulating EPA concentration was >20 times higher than that of the control group which consumed olive oil, indicating that daily consumption, but not a single dose, of α-LA-rich oil might help preserve the physiologic EPA concentration. The consumption of high concentrations of perilla oil for 4 weeks also increased the hepatic expression of Elovl5, which is involved in fatty acid elongation; however, further studies are needed to characterize the relationship between the expression of this gene and the conversion of α-LA to EPA.

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Nutritional composition, heavy metal contents and lipid quality of five marine fish species from Cameroon coast

Manz

Nsoga

Diazenza

et al. 2023

Heliyon

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Selective Consumption of Fish Oil at End of the Day Increases the Physiological Fatty Acid Compositions of Eicosapentaenoic Acid and Docosahexaenoic Acid in Mice

Cited by 4 publications

References 35 publications

Maltose consumption exacerbates high-fat diet-induced overweight and related parameters in mice

Maltose consumption exacerbates high-fat diet-induced overweight and related parameters in mice

Daily Consumption of α-Linolenic Acid Increases Conversion Efficiency to Eicosapentaenoic Acid in Mice

Nutritional composition, heavy metal contents and lipid quality of five marine fish species from Cameroon coast

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