Selective cell retrieval method using light-responsive gas-generating polymer-based microarrays

Abstract

Selective cell retrieval from base material is necessary for developing and improving cell analyzing technologies as well as regenerative medicine. Many conventional technologies, such as micromanipulators, are developed for selective...

Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

“…Once a cell is isolated or selected based on a desired phenotype, it may be necessary to retrieve the cell from its adhering surface for subsequent culture or analysis. Ueno et al proposed a single-cell retrieval method using a polymer microarray that generates gas upon light exposure . The single cell of interest is first identified under a brightfield or fluorescent microscope.…”

“…Ueno et al proposed a singlecell retrieval method using a polymer microarray that generates gas upon light exposure. 100 The single cell of interest is first identified under a brightfield or fluorescent microscope. Then, the irradiation of 365 nm light focused onto the polymer layer made of glycidyl azide polymer and photosensitizer creates nitrogen gas that can effectively displace the single cell from the surface for collection.…”

Integrated Microfluidic Device for Glycated Hemoglobin (HbA1c) Testing Using Light-gas Generation Pump

“…(19) Then, it was possible to detach only one cell by narrowing down the irradiation light. (20,21) In this paper, we describe the development of an integrated microfluidic device for HbA1c measurement. We designed the device using the latex aggregation method, miniaturized it using a mixing channel and a gas-generating tape, and verified its quantitative performance.…”