Selective CDK9 knockdown sensitizes TRAIL response by suppression of antiapoptotic factors and NF-kappaB pathway

Intensity Interrogation-Based High-Sensitivity Surface Plasmon Resonance Imaging Biosensor for Apoptosis Detection in Cancer

“…Western blotting was performed to detect both caspase-3 (CASP-3) zymogen and cleaved/activated caspase-3 (C-CASP-3) in cellular lysates according to a previous description [24]. In brief, A549 cells were first lysed in radio-immunoprecipitation assay (RIPA) buffer containing complete protease inhibitor cocktail (Complete-mini; Roche Diagnostics GmbH, Mannheim, Germany) for extraction of total cellular proteins.…”

“…Once activated by apoptotic signaling, the initiator caspases function to cleave and activate executioner caspases, and the activated executioner caspases cleave hundreds of proteins substrates, leading to apoptosis in cells [26,27]. Caspase-3 is an essential executioner of apoptosis, and its active expression level can be thus employed to reveal apoptosis status in cells [24,28]. As an irreversible caspase-3 inhibitor, the peptide Z-DEVD-FMK specifically binds to C-Casp-3 and suppresses its proteolytic activity [29].…”

Efficient healing of diabetic wounds by MSC-EV-7A composite hydrogel via suppression of inflammation and enhancement of angiogenesis

“…Extracellular vesicles (EVs) are cell-derived natural nanoparticles and vital mediators of cell–cell communications and have been reported as promising platforms for various biomedical applications, such as cancer treatment, 9–12 degenerative neurological disorder therapies 13 and tissue engineering. 14,15 Increasing evidence suggests that mesenchymal stem cell (MSC)-derived EVs are very promising for facilitating skin wound healing.…”