Selective autophagic degradation of ACLY (ATP citrate lyase) maintains citrate homeostasis and promotes oocyte maturation

He, Hainan; Wang, Junling; Mou, Xingmei; Liu, Xin; Li, Qiao; Zhong, Mingyue; Luo, Bingbing; Yu, Zhisheng; Zhang, Jingjing; Xu, Tian; Dou, Cheng-Li; Wu, Dan-Ya; Wei, Qing; Wu, Linhui; Zhou, Kai; Fan, Zhengang; Wang, Tingting; Hu, Taotao; Zhang, Xia; Zhou, Jilong; Miao, Yi‐Liang

doi:10.1080/15548627.2022.2063005

Cited by 14 publications

(7 citation statements)

References 54 publications

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“…ACLY is citric acid lyase, which is an enzyme located upstream of ACC in the TCA cycle (Granchi, 2018). Inhibition of ACLY may reduce the production of Acetyl‐CoA (He et al, 2023). OGDHL is a rate‐limiting enzyme in the TCA cycle that plays an integral role in mitochondrial metabolism, and its downregulation can inhibit the mitochondria of cells for glucose metabolism (Dai et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analyses reveal transcriptional profiles of horse oocytes before and after in vitro maturation

Song,

Jiang,

et al. 2023

Reprod Domestic Animals

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Oocyte in vitro maturation is necessary for the study and application of animal‐assisted reproduction technology in animal reproduction and breeding. The comprehensive transcriptional profile of equine oocyte maturated in vitro has not been fully mined yet, which makes many key transcriptional events still unidentified. Here, Smart‐seq2 was performed to analyse the gene expression pattern and the underlying regulatory mechanism of horse germinal vesicle (GV) and in vitro metaphase II (MII) oocytes. The results showed that 6402 genes (2640 up‐regulated and 3762 down‐regulated in MII samples compared to GV) and 4021 lncRNA transcripts (1210 up‐regulated and 2811 down‐regulated in MII samples compared to GV) were differentially expressed in GV and MII oocytes. Further, GO and KEGG analysis found that differentially expressed mRNAs and lncRNAs were mainly enriched in the pathways related to energy and lipid metabolism. In addition, LGALS3 was found a key gene in mediating the regulation of oocyte meiosis recovery and fertilization ability. This study provides novel knowledge about gene expression and energy metabolism during equine oocyte maturation and a reference for the further study and application of assisted reproductive technology in horse reproduction and breeding.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analyses reveal transcriptional profiles of horse oocytes before and after in vitro maturation

Song,

Jiang,

et al. 2023

Reprod Domestic Animals

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“…Furthermore, citrate has been found to act as a key substrate epigenetic modifications in the oocyte (Gu et al, 2015;Harvey, 2019), while it seems to be play an important role during early embryogenesis, as a positive correlation between citrate concentration in PFF and the number of embryos produced per matured oocyte in humans (Castiglione Morelli et al, 2019). He et al (He et al, 2022) recently revealed that the adverse impact of autophagy impairment on oocyte quality is mediated by downregulated citrate levels, which once enhanced by exogenous supplementation could significantly restore oocyte maturation. They also observed that the decreased autophagy levels in cumulus cells, typical of maternal aging, are related to lower levels of citrate in human PFF, and that elevated citrate levels in porcine PFF can promote oocyte maturation (He et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…He et al (He et al, 2022) recently revealed that the adverse impact of autophagy impairment on oocyte quality is mediated by downregulated citrate levels, which once enhanced by exogenous supplementation could significantly restore oocyte maturation. They also observed that the decreased autophagy levels in cumulus cells, typical of maternal aging, are related to lower levels of citrate in human PFF, and that elevated citrate levels in porcine PFF can promote oocyte maturation (He et al, 2022). Therefore, the lack of citrate in the IVM conditions currently used in donkeys should be addressed by incorporating it in the media.…”

Section: Discussionmentioning

confidence: 99%

Metabolic profiling of preovulatory follicular fluid in jennies

Catalán

Martínez-Rodero²,

Yánez-Ortiz³

et al. 2022

Research in Veterinary Science

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“…The oocyte directs GCs proliferation and differentiation, while GCs influence oocyte maturation. GC is a multifunctional and pivotal component in oocyte maturation by supporting the oocyte development, producing sex steroids, and secreting several growth factors 4,5 . The vitality of GCs is associated with follicle development and oocyte maturation.…”

Section: Introductionmentioning

confidence: 99%

“…GC is a multifunctional and pivotal component in oocyte maturation by supporting the oocyte development, producing sex steroids, and secreting several growth factors. 4,5 The vitality of GCs is associated with follicle development and oocyte maturation. Extensive research has shown that GCs dysfunction plays a significant role in premature ovarian failure, polycystic ovarian syndrome, and some other pathological processes.…”

Section: Introductionmentioning

confidence: 99%

N6‐methyladenosine methyltransferase METTL3 modulates the cell cycle of granulosa cells via CCND1 and AURKB in Haimen goats

Liu,

Zhou,

et al. 2023

The FASEB Journal

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N6‐methyladenosine (m6A) plays a crucial role in many bioprocesses across species, but its function in granulosa cells during oocyte maturation is not well understood in animals, especially domestic animals. We observed an increase in m6A methyltransferase‐like 3 (METTL3) in granulosa cells during oocyte maturation in Haimen goats. Our results showed that knockdown of METTL3 disrupted the cell cycle in goat granulosa cells, leading to aggravated cell apoptosis and inhibition of cell proliferation and hormone secretion. Mechanistically, METTL3 may regulate the cell cycle in goat granulosa cells by mediating Aurora kinase B (AURKB) mRNA degradation in an m6A‐YTH N6‐methyladenosine RNA binding protein 2 (YTHDF2) manner and participating in AURKB transcription via the Cyclin D1 (CCND1)‐Retinoblastoma protein (RB)‐E2F transcription factor 1 (E2F1) pathway. Overall, our study highlights the essential role of METTL3 in granulosa cells during oocyte maturation in Haimen goats. These findings provide a theoretical basis and technical means for understanding how RNA methylation participates in oocyte maturation through granulosa cells.

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Selective autophagic degradation of ACLY (ATP citrate lyase) maintains citrate homeostasis and promotes oocyte maturation

Cited by 14 publications

References 54 publications

Transcriptome analyses reveal transcriptional profiles of horse oocytes before and after in vitro maturation

Transcriptome analyses reveal transcriptional profiles of horse oocytes before and after in vitro maturation

Metabolic profiling of preovulatory follicular fluid in jennies

N6‐methyladenosine methyltransferase METTL3 modulates the cell cycle of granulosa cells via CCND1 and AURKB in Haimen goats

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