Metabolic profiling of preovulatory follicular fluid in jennies

Catalán, Jaime; Martínez-Rodero, Iris; Yánez-Ortiz, Iván; Mateo‐Otero, Yentel; Bragulat, Ana Flores; Nolis, Pau; Carluccio, Augusto; Yeste, Marc; Miró, Jordi

doi:10.1016/j.rvsc.2022.10.026

Cited by 5 publications

(3 citation statements)

References 100 publications

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“…However, the porcine COCs were matured completely in vitro, as opposed to the oocytes and follicular cells of the present study which were stimulated in vivo and, therefore, exposed to follicular fluid for a longer time interval. Follicular fluid concentrations of glutamine have not been determined in mare follicular fluid; however, donkey glutamine follicular fluid concentrations range from 0.25 to 0.74 mM, with a mean concentration of 0.47 mM (Catalán et al, 2022). The concentration is similar to the 0.68 mM L-glutamine and 0.51 mM L-glutamic acid in the culture medium used in the present study.…”

Section: Amino Acids and Derivativesmentioning

confidence: 52%

Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares

Bresnahan,

Catandi,

Peters

et al. 2024

Front. Cell Dev. Biol.

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Introduction: Oocytes and follicular somatic cells within the ovarian follicle are altered during maturation and after exposure to culture in vitro. In the present study, we used a nontargeted metabolomics approach to assess changes in oocytes, cumulus cells, and granulosa cells from dominant, follicular-phase follicles in young and old mares.Methods: Samples were collected at three stages associated with oocyte maturation: (1) GV, germinal vesicle stage, prior to the induction of follicle/oocyte maturation in vivo; (2) MI, metaphase I, maturing, collected 24 h after induction of maturation in vivo; and (3) MIIC, metaphase II, mature with collection 24 h after induction of maturation in vivo plus 18 h of culture in vitro. Samples were analyzed using gas and liquid chromatography coupled to mass spectrometry only when all three stages of a specific cell type were obtained from the same mare.Results and Discussion: Significant differences in metabolite abundance were most often associated with MIIC, with some of the differences appearing to be linked to the final stage of maturation and others to exposure to culture medium. While differences occurred for many metabolite groups, some of the most notable were detected for energy and lipid metabolism and amino acid abundance. The study demonstrated that metabolomics has potential to aid in optimizing culture methods and evaluating cell culture additives to support differences in COCs associated with maternal factors.

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Section: Amino Acids and Derivativesmentioning

confidence: 52%

Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares

Bresnahan,

Catandi,

Peters

et al. 2024

Front. Cell Dev. Biol.

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“…For instance, proline has been shown to increase the rate of development and maturation of bovine oocytes in vitro (Bahrami et al., 2023). Likewise, proline was found to be present in the pre‐ovulatory follicular fluid of jennines, suggesting its role in the development and maturation of oocytes (Catalán et al., 2022). The increased level of proline during the proestrus stage might be related to the extracellular matrix (ECM) turnover in the cells.…”

Section: Discussionmentioning

confidence: 99%

Taurine dynamics in serum during the oestrous cycle in buffaloes

Joshi,

Kumar,

Singh

et al. 2024

Reprod Domestic Animals

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Estrus identification is one of the common issues in buffaloes because of their short estrus duration and silent estrus problem. Hence, specific biomarkers facilitating in identifying the estrus stage would be helpful to buffalo farmers and researchers. In our previous studies, taurine, a non‐protein amino acid that helps in the secretion of reproductive hormones such as GnRH, was found to be associated with postpartum anestrus in buffaloes. Therefore, the present study was conducted to explore the level of taurine in serum during different stages of the oestrous cycle in healthy cyclic buffaloes. Blood samples were collected from healthy cyclic buffaloes (n = 4), and taurine was estimated at the estrus (0th day), proestrus (−2nd day), metestrus (3rd day) and diestrus (+10th day) stages using TLC method. The days of the oestrous cycle were determined by ultrasonography and observation of behavioural signs by trained professionals. The results revealed that taurine was consistently present in the serum. However, the highest concentration of taurine was observed at the proestrus (0.20 ± 0.03 mg/mL) stage, which was greater (p < .05) than metestrus (0.10 ± 0.05 mg/mL) and diestrus (0.13 ± 0.03 mg/mL) stages, but comparable with the estrus stage. These results were also validated in the simulated population datasets of population size 6 to 10,000. Further, ROC curve analysis for the large simulated population indicated the efficiency of taurine to distinguish proestrus from metestrus and diestrus stages at a lower cutoff value of <0.1643 mg/mL with 60% sensitivity and specificity. Therefore, the present study concludes that serum taurine concentration could help in detecting proestrus stage of buffalo estrous cycle.

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“…Granulosa cells provide sufficient amounts of pyruvate, lactic acid, and nicotinamide adenine dinucleotide phosphate (NADPH) to oocytes through glycolysis and the tricarboxylic acid cycle. Oocytes produce the ATP required for oocyte maturation through the oxidative phosphorylation (OXPHOS) pathway [ 37 ]. While SIRT1, one of the proteins highly expressed in granulosa cells, has a relevant role in regulating energy metabolism and mitochondrial function, this regulation requires the involvement of an important energy sensor, AMPK.…”

Section: Regulation Of Oxidative Stress and Mitochondrial Function By...mentioning

confidence: 99%

Regulation of SIRT1 in Ovarian Function: PCOS Treatment

et al. 2023

CIMB

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The sirtuin family, a group of NAD+-dependent class 3 histone deacetylases (HDACs), was extensively studied initially as a group of longevity genes that are activated in caloric restriction and act in concert with nicotinamide adenine dinucleotides to extend the lifespan. Subsequent studies have found that sirtuins are involved in various physiological processes, including cell proliferation, apoptosis, cell cycle progression, and insulin signaling, and they have been extensively studied as cancer genes. In recent years, it has been found that caloric restriction increases ovarian reserves, suggesting that sirtuins may play a regulatory role in reproductive capacity, and interest in the sirtuin family has continued to increase. The purpose of this paper is to summarize the existing studies and analyze the role and mechanism of SIRT1, a member of the sirtuin family, in regulating ovarian function. Research and review on the positive regulation of SIRT1 in ovarian function and its therapeutic effect on PCOS syndrome.

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Metabolic profiling of preovulatory follicular fluid in jennies

Cited by 5 publications

References 100 publications

Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares

Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares

Taurine dynamics in serum during the oestrous cycle in buffaloes

Regulation of SIRT1 in Ovarian Function: PCOS Treatment

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