Selective and sensitive detection of L-Cysteine via fluorometric assay using gold nanoparticles and Rhodamine B in aqueous medium

Maiti, Pradip; Singha, Tanmoy; Chakraborty, Utsav; Roy, Somnath; Karmakar, Parimal; Dey, Bapi; Hussain, Syed Arshad; Paul, Sharmistha; Paul, Piyali

doi:10.1016/j.matchemphys.2019.06.001

Cited by 16 publications

(20 citation statements)

References 39 publications

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“…Generally, the process of chemisorptions limit to the interaction between disulfides (S–S) or thiol (R–SH) compound and Au NPs. ,− It should be noted that disulfide is much more strongly bound to the gold surface compared with thiol. The very weak interactions between other S-containing pesticides and Au NPs maybe ascribed to the less accessible intra-bond S-atom . On the other hand, for N-containing pesticides, the interactions between them and Au NPs are explained for the electrostatic interaction and not chemisorptions .…”

Section: Results and Discussionmentioning

confidence: 98%

“…The very weak interactions between other S-containing pesticides and Au NPs maybe ascribed to the less accessible intra-bond S-atom. 45 On the other hand, for N-containing pesticides, the interactions between them and Au NPs are explained for the electrostatic interaction and not chemisorptions. 46 Therefore, the Ncontaining pesticides in the study could not cause the aggregation of Au NPs, which may be attributed to the weak electrostatic attraction.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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A Portable Smartphone Platform Using a Ratiometric Fluorescent Paper Strip for Visual Quantitative Sensing

Chu

Wang

Xiao

et al. 2020

ACS Appl. Mater. Interfaces

230

101

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Instrument-free, portable, and direct read-out minidevices have wider application prospects in various fields, especially for real-time/on-site sensing. Herein, combined with a paper strip, a smartphone sensing platform integrated with a UV lamp and dark cavity by 3D-printing technology has been developed for the rapid, sensitive, instrument-free, and visual quantitative analysis in real-time/on-site conditions. The platform proved the feasibility for visual quantitative detection of pesticide via a fluorescence "on−off−on" response with a single dualemissive ratiometric paper strip. Red-emitting CdTe quantum dots (rQDs) were embedded into the silica nanoparticles (SiO 2 NPs) as an internal reference, while blue-emitting carbon dots (bCDs) as a signal report unit were covalently linked to the outer surface of SiO 2 NPs. The blue fluorescence could be quenched by gold nanoparticles (Au NPs) and then recovered with pesticide. The red (R), green (G), and blue (B) channel values of the generated images were determined by a color recognizer application (APP) installed in the smartphone, and the R/B values could be used for pesticide quantification with a sensitive detection limit (LOD) of 59 nM. The smartphone sensing platform based on 3D printing might provide a general strategy for visual quantitative detection in a variety of fields including environments, diagnosis, and safety monitoring.

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Section: Results and Discussionmentioning

confidence: 98%

Section: ■ Results and Discussionmentioning

confidence: 99%

A Portable Smartphone Platform Using a Ratiometric Fluorescent Paper Strip for Visual Quantitative Sensing

Chu

Wang

Xiao

et al. 2020

ACS Appl. Mater. Interfaces

230

101

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“…In the selectivity test, other S-containing atoms have almost no effect because it is difficult for the S atoms in the bond to come into contact with the surface of Au NPs. 38 For pesticides containing N atoms, the interaction with Au NPs should be attributed to the electrostatic interaction rather than the chemical adsorption process. 39 Meanwhile, with addition of the mixture of thiram and the other compounds, we can see that the chemiluminescence signal is almost unchanged compared with addition of only thiram, which proves its excellent anti-interference ability (Figure S7B).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The result shows that thiram chemically adsorbs onto the surface of Au NPs strongly and quickly, and a chemical adsorption process usually exists between disulfide (S–S) and mercaptans (R–SH), − proving that the effect between disulfide and Au NPs is stronger. In the selectivity test, other S-containing atoms have almost no effect because it is difficult for the S atoms in the bond to come into contact with the surface of Au NPs . For pesticides containing N atoms, the interaction with Au NPs should be attributed to the electrostatic interaction rather than the chemical adsorption process .…”

Section: Resultsmentioning

confidence: 99%

Gold Nanoparticle-Based Peroxyoxalate Chemiluminescence System for Highly Sensitive and Rapid Detection of Thiram Pesticides

Lin

Yang

et al. 2021

ACS Appl. Nano Mater.

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Abuse and inappropriate disposal of the pesticide thiram can be hazardous to human health and sustainable development of the environment. Herein, we designed a peroxyoxalate chemiluminescence-based nanosensing system composed of bis(2,4,6-trichlorophenyl)oxalate (TCPO), hydrogen peroxide (H2O2), and specific-sized gold nanoparticles (Au NPs) for highly sensitive, rapid, and selective detection of thiram pesticide via a chemiluminescence “on–off” phenomenon. The nanoscale sensing system produced strong chemiluminescence through excited specific-sized Au NPs by the energy transfer from peroxyoxalate chemically; however, the chemiluminescence was very sensitive to thiram and decreased gradually because the discrete energy level of Au NPs changed after interacting with thiram to form an aggregated complex through intense Au–S bonds. The proposed chemiluminescence-based nanosensor could achieve the determination of thiram with a sensitive and low limit of detection (LOD) of 0.35 nM, within a detection time of 40 s. The nanoscale sensing system reported herein was simple, rapid, low cost, and highly sensitive and selective for sulfhydryl-containing environmentally hazardous substances like thiram pesticide residues in fruits, vegetables, milk, and environmental water, promoting the development and application of nanomaterial-based chemiluminescence sensing in the fields of food and environment safety monitoring.

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“…The high extinction coefficient of Au NPs in the visible region renders them as an efficient platform for signaling molecular recognition processes through their color change. 21 The small sizes of these metal NPs with a large surface curvature facilitate their binding with various organic dyes, proteins 16 , 18 etc. The degree of structural perturbation of the protein molecules due to their interaction with NPs varies for different protein species.…”

Section: Introductionmentioning

confidence: 99%

Comparative and Selective Interaction of Amino Acid d-Cysteine with Colloidal Gold Nanoparticles in the Presence of a Fluorescent Probe in Aqueous Medium

et al. 2022

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In this communication, we report the comparative and selective interaction of amino acid d -cysteine ( d -Cys) with citrate caped gold nanoparticles (Au NPs) in the presence of a fluorescent dye, rhodamine B (RhB), in aqueous solution. Au NPs of size 27.5 nm could almost fully quench the steady-state fluorescence emission of RhB at their optimum concentrations in the mixed solution. The interactions of d -Cys, l -Cys, all other relevant d - and l -amino acids, neurotransmitters, and other relevant biological compounds with the Au NPs/RhB mixed solution have been explored by monitoring the fluorescence recovery efficiencies from the almost fully quenched state of RhB fluorescence via a simple steady-state spectrofluorometric method. The higher fluorescence recovery for the interaction of d -Cys with the Au NPs/RhB mixed system is accompanied by a distinct color change (red-wine to bluish-black) of the assay medium after the reaction compared to that of all other interfering compounds considered in this work. The sensitivity of this fluorometric response lies in a broad linear range of concentrations of d -Cys and the limit of detection (LOD) is found to be 4.2 nM, which is low compared to many other methods available in the literature. The different degrees of interaction of d -Cys and l -Cys with the Au NPs/RhB mixed sample have been further explored by circular dichroism (CD) spectroscopy and Fourier transform infrared (FTIR) spectroscopy. The selective interaction of d -Cys with the proposed Au NPs/RhB mixed system is also found to be correlated with interparticle cross-linking and aggregations of nanoparticles by the analysis of ζ potential and dynamic light scattering (DLS) study, transmission electron microscopy (TEM), atomic force microscopy (AFM), UV–vis absorption spectroscopy etc. The proposed interaction mechanism is further studied with a normal human urine sample to elucidate that the optimized combination of Au NPs and RhB may be realized as an efficient platform for detection of the amino acid d -Cys in a real biosample via a simple fluorometric approach.

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Selective and sensitive detection of L-Cysteine via fluorometric assay using gold nanoparticles and Rhodamine B in aqueous medium

Cited by 16 publications

References 39 publications

A Portable Smartphone Platform Using a Ratiometric Fluorescent Paper Strip for Visual Quantitative Sensing

A Portable Smartphone Platform Using a Ratiometric Fluorescent Paper Strip for Visual Quantitative Sensing

Gold Nanoparticle-Based Peroxyoxalate Chemiluminescence System for Highly Sensitive and Rapid Detection of Thiram Pesticides

Comparative and Selective Interaction of Amino Acid d-Cysteine with Colloidal Gold Nanoparticles in the Presence of a Fluorescent Probe in Aqueous Medium

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