Selective and Accurate Determination Method of Propofol in Human Plasma by Mixed-Mode Cation Exchange Cartridge and GC-MS

Pyo, Jae Sung

doi:10.1155/2016/9531769

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…In addition, the LODs required for several applications are within reach of the TLC/LTP-MS method developed here. For example, the LOD of propofol was determined to be 0.77 ng/mm 2 , which is equivalent to 3.7 μg mL –1 if the volume of standard solution spotted on the TLC plate (2 μL) is taken into account, while the therapeutic dose of propofol is 6–10 μg mL –1 for induction of anesthesia . While high uncertainty may result from the LOD being close to the application’s low end range requirements, fast and simple preconcentration methods, recently demonstrated for propofol, are readily available. , Also, the LOD for nicotine was determined as 0.20 ng/mm 2 (∼1.9 ng per sample spot), which shows better performance than the reported LOD of 2 μg for determination of nicotine in cigarettes by the SPME/GC-MS method .…”

Section: Resultsmentioning

confidence: 99%

Low-Temperature Plasma Probe Mass Spectrometry for Analytes Separated on Thin-Layer Chromatography Plates: Direct vs Laser Assisted Desorption

Gong

Zhang

Embile

et al. 2020

J. Am. Soc. Mass Spectrom.

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Thin-layer chromatography (TLC) is a widespread technique because it allows fast, simple, and inexpensive analyte separations. In addition, direct analysis of the compounds separated on TLC plates via mass spectrometry (MS) has been shown to provide high sensitivity and selectivity while avoiding time-consuming sample extraction protocols. Here, direct desorption low-temperature plasma-mass spectrometry (LTP-MS) as well as diode laser assisted desorption (LD) LTP-MS are studied for direct spatially resolved analysis of compounds from TLC plates. Qualitative and quantitative characterization of amino acids, pharmaceuticals, and structural isomers were performed. The nature of the TLC plate stationary phase was found to have a significant influence, together with the analyte's characteristics, on the desorption efficiency. Tandem MS is shown to greatly improve the limits of detection (LODs). Direct desorption LTP-MS, without external thermal assisted desorption, demonstrates its best performance with cellulose TLC plates (LODs, 0.01 ng/mm 2 to 2.55 ng/mm 2 ) and restricted performance with normal-phase (NP) TLC plates (several analytes without observable signal). LD LTP-MS, with systematic optimization of irradiance and focal point diameter, is shown to overcome the direct-desorption limitations and reach significantly improved LODs with NP TLC plates (up to ×1000 better). In addition, a wide-ranging characterization of amino acid analytical figures of merit with LD LTP-MS shows that LODs from 84 pg/mm 2 down to 0.3 pg/mm 2 are achieved on NP TLC plates.

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Section: Resultsmentioning

confidence: 99%

Low-Temperature Plasma Probe Mass Spectrometry for Analytes Separated on Thin-Layer Chromatography Plates: Direct vs Laser Assisted Desorption

Gong

Zhang

Embile

et al. 2020

J. Am. Soc. Mass Spectrom.

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“…To date, propofol detection methods include, gas chromatography-mass spectrometry (GC-MS) [29], liquid chromatography-mass spectrometry (LC-MS) [30], high-performance liquid chromatography (HPLC) [31], capillary electrophoresis [32], fluorophotometry [33] and UV-Vis spectrophotometry [34][35][36]. Fluorophotometry is a simple, sensitive and easily detectable technique [37].…”

Section: Introductionmentioning

confidence: 99%

Graphene quantum dots as nanoprobes for fluorescent detection of propofol in emulsions

Diao

Wang

2019

R. Soc. open sci.

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We report a new fluorescent detection method for propofol based on graphene quantum dots (GQDs). Citric acid (CA) was selected as the carbon precursor, and fluorescent GQDs were prepared by carbonizing CA. The product, which efficiently quenched the fluorescence of GQDs, could be obtained through the oxidation of propofol in the presence of horseradish peroxidase and hydrogen peroxide. The fluorescence intensity ratio of GQDs (F/F0) was positively correlated with the concentration of propofol, which ranged within 5.34–89.07 mg l−1, the limit of detection was 0.5 mg l−1 and the limit of quantity was 5.34 mg l−1. The developed fluorescence method reported in the present study is simple, sensitive, reproducible, and can serve in determining propofol contents in emulsions.

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“…The retention time of toluene was 6.03 minutes with the major ion m/z of 91. Propofol produces 3 main glucuronide conjugates: propofol‐glucuronide, 1‐quinol‐glucuronide, and 4‐quinol‐glucuronide; however, there was no evidence of these highly polar compounds in the plasma samples 20 …”

Section: Resultsmentioning

confidence: 99%

Unique volatile metabolite signature of sinonasal inverted papilloma detectable in plasma and nasal secretions

Chaskes

Lee

Toskala

et al. 2022

Int Forum Allergy Rhinol

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Background: Sinonasal inverted papilloma (SNIP) is a benign neoplasm with aggressive features, including a high recurrence rate and a propensity for malignant transformation. Accurate diagnosis with complete resection and the need for close long-term surveillance is widely accepted as standard management. In this study, we investigate whether SNIP produces a unique volatile metabolite signature, which may ultimately lead to a novel approach to diagnose and monitor SNIP. Methods: Whole blood and nasal secretions from patients with SNIP and healthy age-, sex-, and smoking-status-matched controls, were collected. There were 56 blood samples and 42 nasal secretion samples collected. The volatile metabolite signature of SNIP plasma and nasal secretion samples were compared with those of healthy controls using chromatography. Results: Seventy-two volatiles were identified in plasma samples. Multivariate analysis of variance results, even when controlled for smoking status, indicated toluene as a significant univariate result with lower levels of toluene identified in SNIP plasma samples than healthy control plasma samples. A linear discriminant analysis (LDA) model for plasma volatiles correctly classified 23 of 24 SNIP patients and 26 of 27 control patients, with a cross-validation error rate of 6.02%. Sixty-nine volatiles were identified in nasal samples. For nasal secretion samples, no single univariate response was significant. The LDA model correctly classified 21 of 21 SNIP patients and 11 of 12 control patients, with a cross-validation error rate of 6.55%. Conclusion:This study suggests that SNIP produces a unique, detectable volatile metabolite signature. With further investigation, this can have dramatic clinical implications for diagnosis and monitoring. Although most volatile metabolite studies have investigated solid-organ malignancy, this novel study addresses a benign sinonasal neoplasm by using nasal secretions and plasma as an analysis medium, representing the first such study.

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Selective and Accurate Determination Method of Propofol in Human Plasma by Mixed-Mode Cation Exchange Cartridge and GC-MS

Cited by 7 publications

References 14 publications

Low-Temperature Plasma Probe Mass Spectrometry for Analytes Separated on Thin-Layer Chromatography Plates: Direct vs Laser Assisted Desorption

Low-Temperature Plasma Probe Mass Spectrometry for Analytes Separated on Thin-Layer Chromatography Plates: Direct vs Laser Assisted Desorption

Graphene quantum dots as nanoprobes for fluorescent detection of propofol in emulsions

Unique volatile metabolite signature of sinonasal inverted papilloma detectable in plasma and nasal secretions

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