Selective Analysis of Lipids by Thin-Layer Chromatography Blot Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry

Zaima, Nobuhiro; Goto‐Inoue, Naoko; Adachi, Kohsuke; Setou, Mitsutoshi

doi:10.5650/jos.60.93

Cited by 45 publications

(30 citation statements)

References 27 publications

(20 reference statements)

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“…In the previous TLC-blot-MALDI-IMS, PC (18:0/22:6) was detected as [M+K] + having an m/z of 872.5 ( 34 ) and PE (18:0/22:6) was detected as [M+2Na-H] + having an m/z of 836.6 ( 35 ). As shown in Fig.…”

Section: Lipid Analyses By Tlc and Determination Of Pc (18:0/22:6) Anmentioning

confidence: 97%

PGC-1α-mediated changes in phospholipid profiles of exercise-trained skeletal muscle

Senoo

Miyoshi

Goto‐Inoue

et al. 2015

Journal of Lipid Research

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This article is available online at http://www.jlr.org infl uences cell permeability and receptor stability at the cell membrane, these phospholipids may contribute to exercise training-mediated functional changes in the skeletal muscle. Phospholipids are important structural components of membranes, and they infl uence a number of physical properties related to membrane function, including fl uidity, permeability, and the anchoring of membrane-related proteins. Because altering dietary fatty acids ( 1-3 ) and Abstract Exercise training infl uences phospholipid fatty acid composition in skeletal muscle and these changes are associated with physiological phenotypes; however, the molecular mechanism of this infl uence on compositional changes is poorly understood. Peroxisome proliferator-activated receptor ␥ coactivator 1 ␣ (PGC-1 ␣ ), a nuclear receptor coactivator, promotes mitochondrial biogenesis, the fi ber-type switch to oxidative fi bers, and angiogenesis in skeletal muscle. Because exercise training induces these adaptations, together with increased PGC-1 ␣ , PGC-1 ␣ may contribute to the exercise-mediated change in phospholipid fatty acid composition. To determine the role of PGC-1 ␣ , we performed lipidomic analyses of skeletal muscle from genetically modified mice that overexpress PGC-1 ␣ in skeletal muscle or that carry KO alleles of PGC-1 ␣ . We found that PGC-1 ␣ affected lipid profi les in skeletal muscle and increased several phospholipid species in glycolytic muscle, namely phosphatidylcholine (PC) (18:0/22:6) and phosphatidylethanolamine (PE) (18:0/22:6). We also found that exercise training increased PC (18:0/22:6) and PE (18:0/22:6) in glycolytic muscle and that PGC-1 ␣ was required for these alterations. Because phospholipid fatty acid composition

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Section: Lipid Analyses By Tlc and Determination Of Pc (18:0/22:6) Anmentioning

confidence: 97%

PGC-1α-mediated changes in phospholipid profiles of exercise-trained skeletal muscle

Senoo

Miyoshi

Goto‐Inoue

et al. 2015

Journal of Lipid Research

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“…This information is very important if we are to elucidate the mechanisms of action of anthocyanins; to date, however, methods for investigating anthocyanin localization, for example determining specific antibodies to each anthocyanin species, have not yet been developed. MALDI-IMS is an emerging technique that enables simultaneous investigation of both the content and spatial distribution of a wide range of biomolecules, for example lipids [17][18][19], glycolipids [20,21], amino acids [22], proteins [23], peptides [24,25], and administered pharmaceuticals [26], without requiring antibodies, staining, or complicated pretreatment steps.…”

Section: Introductionmentioning

confidence: 99%

Visualization of anthocyanin species in rabbiteye blueberry Vaccinium ashei by matrix-assisted laser desorption/ionization imaging mass spectrometry

et al. 2012

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Anthocyanins are naturally occurring compounds that impart color to fruits, vegetables, and plants, and are believed to have a number of beneficial health effects in both humans and animals. Because of these properties, pharmacokinetic analysis of anthocyanins in tissue has been performed to quantify and identify anthocyanin species although, currently, no methods exist for investigating tissue localization of anthocyanin species or for elucidating the mechanisms of anthocyanin activity. Imaging mass spectrometry (IMS) is powerful tool for determining and visualizing the distribution of a wide range of biomolecules. To investigate whether anthocyanin species could be identified and visualized by IMS, we performed matrix-assisted laser desorption/ionization (MALDI)-IMS analysis, by tandem mass spectrometry (MALDI-IMS-MS), of ten anthocyanin molecular species in rabbiteye blueberry (Vaccinium ashei). The distribution patterns of each anthocyanin species were different in the exocarp and endocarp of blueberry sections. Anthocyanin species composed of delphinidin and petunidin were localized mainly in the exocarp. In contrast, those species composed of cyanidin, peonidin, and malvidin were localized in both the exocarp and the endocarp. Moreover, MALDI-IMS analysis of anthocyanidins in a blueberry section indicated that the distribution patterns of each anthocyanidin species were nearly identical with those of the corresponding anthocyanins. These results suggested that the different distribution patterns of anthocyanin species in the exocarp and endocarp depended on the aglycone rather than on the sugar moieties. This study is the first to visualize anthocyanin molecular species in fruits.

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“…MALDI-TOF-IMS Analysis and localization of bioactive nutrients (ginsenosides and capsaicin) [222] MALDI-TOF-IMS Visualization of anthocyanin species in rabbiteye blueberry Vaccinium ashei [223] MALDI-TOF-IMS Different localization patterns of anthocyanin species in the pericarp of black rice [224] TLC-Blot-MALDI-TOF-IMS Selective analysis of lipids [225] MALDI-TOF-IMS Oryza sativa (rice) [226] MALDI-TOF-IMS Visualization of spatial distribution of γ-aminobutyric acid in eggplant (Solanum melongena) [227] MALDI-TOF-IMS Unambiguous characterization and tissue localization of Pru P 3 peach allergen [228] LA-ICP-TOF-IMS Imaging of nutrient elements in the leaves of Elsholtzia splendens [229] LAESI-TOF-IMS Macroscopic and microscopic imaging of pesticides, mycotoxins, and plant metabolites on rose leaves, orange and lemon fruits, ergot bodies, cherry tomatoes, and maize kernels [230] SPI-LDI-IMS Simultaneous in situ assessment of aflatoxins and stilbenes [221] LDI-SIMS-IMS Comparison of sample pretreatments for the imaging of Miscanthus × giganteus [220] LDI-SIMS-IMS Localization of flavonoids in seed sections of peas (Pisum sativum) and Arabidopsis thaliana [231] LA-ICP-MS, laser ablation inductively coupled plasma mass spectrometry; LAESI-TOF-MS, laserablation electrospray ionization time of flight mass spectrometry imaging; LDI-SIMS-IMS, laser desorption/ionization secondary ion mass spec trometry imaging; MALDI-TOF-IMS, matrix-assisted laser desorption/ionization time of flight imaging mass spectrom etry; SPI-LDI-QqTOF-IMS, silica plate imprinting laser desorption/ionization imaging mass spectrometry; TLC-Blot-MALDI-TOF-IMS, thin-layer chromatography blotting matrix-assisted laser desorption/ ionization time of flight imaging mass spectrometry.…”

Section: Type Application Referencesmentioning

confidence: 99%

Advanced Mass Spectrometry

Picó

2015

Advanced Mass Spectrometry for Food Safety and Quality

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Selective Analysis of Lipids by Thin-Layer Chromatography Blot Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry

Cited by 45 publications

References 27 publications

PGC-1α-mediated changes in phospholipid profiles of exercise-trained skeletal muscle

PGC-1α-mediated changes in phospholipid profiles of exercise-trained skeletal muscle

Visualization of anthocyanin species in rabbiteye blueberry Vaccinium ashei by matrix-assisted laser desorption/ionization imaging mass spectrometry

Advanced Mass Spectrometry

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