As a result of multi-year trial (2012/13-2016/17) there have been proved statistically significant breeding and genetic improvement of yield, its stability and level of manifestation yield-related traits in new winter barley varieties Paladin Myronivskyi, MIP Yason, MIP Oskar, and MIP Hladiator compared to the old Myronivka variety Bemir 2. The winter barley varieties MIP Yason and Paladin Myronivskyi were characterized with optimal combination of yield and its stability according to the AMMI model. When using statistical and graphical analysis, the winter barley varieties MIP Hladiator and MIP Oskar were revealed to have the highest genetic gain for kernel number per spike, Paladin Myronivskyi for number of productive tillers and MIP Yason number of productive tillers and thousand kernel weight. Thus, the newly developed winter barley varieties differed from each other with their combination of yield and its components. According to the GYT biplot model the winter barley variety MIP Yason was the nearest to the “ideal” genotype by yield*traits combination.