Selection of Type I and Type II methanotrophic proteobacteria in a fluidized bed reactor under non-sterile conditions

Pfluger, Andrew; Wu, Wei‐Min; Pieja, Allison J.; Wan, Jonathan; Rostkowski, Katherine H.; Criddle, Craig S.

doi:10.1016/j.biortech.2011.08.054

Cited by 59 publications

(33 citation statements)

References 29 publications

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“…N 2 or NO 3 − ), which explains these seemingly contradictory results. For example, type II methanotrophs dominated when NO 3 − -N was limiting and O 2 concentrations were low (Graham et al, 1993;Pfluger et al, 2011). In contrast, maximum PHA/PHB content of 42% was achieved by Rahnama et al (2012) when cultivating Methylocystis hirsute at a CH 4 to O 2 ratio of 50/50 using modified NMS medium supplemented with 50% NO 3 − as N source.…”

Section: Effect Of Ph and Temperaturementioning

confidence: 96%

“…In addition, operational sequences such as single or two phase-systems, semi-continuous or continuous modes were also tested (Zuniga et al, 2011;Pieja et al, 2012). Pfluger et al (2011) used high-rate, aerobic, biological fluidized-bed-reactors (FBRs) with inert packing materials (granular activated carbon) to support the growth of biofilms and maximize PHA/PHB yields. In a recent study, bubble-column reactors using a novel system design, i.e., force-liquid vertical tubular loop bioreactors were tested for PHA/PHB production (Rahnama et al, 2012).…”

Section: Factors Affecting Bioaccumulation Of Pha/phb In Type II Methmentioning

confidence: 99%

“…In a recent study, bubble-column reactors using a novel system design, i.e., force-liquid vertical tubular loop bioreactors were tested for PHA/PHB production (Rahnama et al, 2012). Overall, PHA/PHB yields from different reactor configurations varied between 10% and 52% (Han et al, 2009;Pfluger et al, 2011;Zuniga et al, 2011;Pieja et al, 2012;Rahnama et al, 2012;van der Ha et al, 2012). The high variability was mainly attributed to strains/mixed consortia used in the different studies, operational sequences, testing conditions, and bioreactor/biofilter design.…”

Section: Factors Affecting Bioaccumulation Of Pha/phb In Type II Methmentioning

confidence: 99%

See 2 more Smart Citations

Review of Sustainable Methane Mitigation and Biopolymer Production

Karthikeyan

Chidambarampadmavathy

Cirés

et al. 2014

Critical Reviews in Environmental Science and Technology

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Biological methane (CH 4 ) mitigation combined with biopolymer (PHA-polyhydroxyalkaonate/PHB-polyhydroxybutyrate) production is a viable option in this fossil fuel-constrained era.Methantrophs are bacteria that can re-route CH 4 into PHA/PHB under nutrient-starved conditions. However, most studies (up to 90%) investigated pure cultures to demonstrate capacity for PHA/PHB accumulation, which on an industrial scale is unlikely to be serviceable. Furthermore, commercialization is handicapped as there are still a number of unresolved issues which affect productivities, such as optimized process variables, characterization of robust consortia, and optimized reactor design. This review will summarize existing knowledge and highlight research needs to fast track methanotrophic PHA/PHB production from CH 4 .

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Section: Effect Of Ph and Temperaturementioning

confidence: 96%

Section: Factors Affecting Bioaccumulation Of Pha/phb In Type II Methmentioning

confidence: 99%

Section: Factors Affecting Bioaccumulation Of Pha/phb In Type II Methmentioning

confidence: 99%

See 1 more Smart Citation

Review of Sustainable Methane Mitigation and Biopolymer Production

Karthikeyan

Chidambarampadmavathy

Cirés

et al. 2014

Critical Reviews in Environmental Science and Technology

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“…The improvement of mass transfer limitation could be optimize by the use of high-rate reactor configurations (i.e., pack-bed columns, fluidised bed reactors). By way of example, Pfluger et al (2011) enriched a methanotrophic community from a hot spring sediment for PHB production in a fluidized bed reactor, resulting in high-density biofilms achieving up to 20–40% of PHB by dry cell under nitrogen absence. Finally, an unexplored alternative to decrease the methane mass transfer limitation could be to integrate the eﬄuent of AnMBR, rich in dissolved methane using high-rate reactors, to enrich and accumulate PHA from methanotrophs.…”

Section: Resource Recovery For a Circular Economymentioning

confidence: 99%

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

et al. 2017

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Limits in resource availability are driving a change in current societal production systems, changing the focus from residues treatment, such as wastewater treatment, toward resource recovery. Biotechnological processes offer an economic and versatile way to concentrate and transform resources from waste/wastewater into valuable products, which is a prerequisite for the technological development of a cradle-to-cradle bio-based economy. This review identifies emerging technologies that enable resource recovery across the wastewater treatment cycle. As such, bioenergy in the form of biohydrogen (by photo and dark fermentation processes) and biogas (during anaerobic digestion processes) have been classic targets, whereby, direct transformation of lipidic biomass into biodiesel also gained attention. This concept is similar to previous biofuel concepts, but more sustainable, as third generation biofuels and other resources can be produced from waste biomass. The production of high value biopolymers (e.g., for bioplastics manufacturing) from organic acids, hydrogen, and methane is another option for carbon recovery. The recovery of carbon and nutrients can be achieved by organic fertilizer production, or single cell protein generation (depending on the source) which may be utilized as feed, feed additives, next generation fertilizers, or even as probiotics. Additionlly, chemical oxidation-reduction and bioelectrochemical systems can recover inorganics or synthesize organic products beyond the natural microbial metabolism. Anticipating the next generation of wastewater treatment plants driven by biological recovery technologies, this review is focused on the generation and re-synthesis of energetic resources and key resources to be recycled as raw materials in a cradle-to-cradle economy concept.

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“…Different configurations of closed bioreactors, like traditional biofilters and biotrickling filters (BTFs), have been tested looking for an improved configuration that allows a better control of the factors that determine the rate of CH 4 bio-oxidation (Nikiema et al 2009;Rocha-Ríos et al 2009;Pfluger et al 2011;Veillete et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Oxidation of methane in biotrickling filters inoculated with methanotrophic bacteria

Cáceres

Dorado

Gentina

et al. 2016

Environ Sci Pollut Res

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The oxidation of methane (CH 4 ) using biofilters has been proposed as an alternative to mitigate anthropogenic greenhouse gas emissions with low concentration of CH 4 that cannot be used as a source of energy. However conventional biofilters utilize organic packing materials that have a short lifetime, clogging problems and are commonly inoculated with non-specific microorganisms leading to unpredictable CH 4 elimination capacities (EC) and removal efficiencies (RE). The main objective of this work was to characterize the oxidation of CH 4 in two biotrickling filters (BTFs) packed with polyethylene rings and inoculated with two methanotrophic bacteria Methylomicrobium album and Methylocystis sp. in order to determine the CH 4 elimination capacity (EC) and CO 2 production (pCO 2 ) when using a specific inoculum. The repeatability of the results in both BTF was determined when operated at the same inlet load of CH 4 . A dynamic mathematical model that describes the CH 4 abatement in the BTFs was developed and validated using mass transfer and kinetic parameters estimated independently. Results showed that EC and pCO 2 of the BTFs are not identical but very similar at all the conditions tested. The use of specific inoculum has shown a faster start-up and higher EC per unit area (0.019 g CH4 ·m -2 ·h -1 ) in comparison to most of previous studies at the same CH 4 load rate (23.2 g CH4 ·m -3 ·h -1 ). Global mass balance shown that the maximum reduction of CO 2 equivalents was 98.5 g CO2eq ·m -3 ·h -1 . Model developed described satisfactorily CH 4 abatement in BTFs in a wide range of conditions.

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Selection of Type I and Type II methanotrophic proteobacteria in a fluidized bed reactor under non-sterile conditions

Cited by 59 publications

References 29 publications

Review of Sustainable Methane Mitigation and Biopolymer Production

Review of Sustainable Methane Mitigation and Biopolymer Production

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

Oxidation of methane in biotrickling filters inoculated with methanotrophic bacteria

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