Selection of stable banana clones which do not produce dwarf somaclonal variants during in vitro culture

Israeli, Y.; Ben-Bassat, D.; Reuveni, O.

doi:10.1016/s0304-4238(96)00955-7

Cited by 29 publications

(17 citation statements)

References 13 publications

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“…Williams (Cavendish banana), have been reported (Israeli et al 1996) and such dwarf somaclones were relatively stable and did not generally revert to a normal phenotype (Ramage et al 2004). It has been reported in Musa that the extent of instability caused by in vitro culture was related to cultivar rather than the culture condition (Ray et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers

Lakshmanan¹,

Venkataramareddy²,

Neelwarne³

2007

Electron. J. Biotechnol.

150

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A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cultures with distinct variation in morphological characteristics (morphotypes). Similarly, the mother maintained in the field was also subjected for genetic analysis. Out of the 50 RAPD and 25 ISSR primers screened, 30 RAPD and 5 ISSR primers produced totally 424 clear, distinct and reproducible band classes resulting in a total of 5088 bands where the banding patterns for each primer was highly uniform and comparable to the field-grown *Corresponding author mother clone from which the cultures had been established. These results indicate that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and applicable for clonal propagation of banana var. NR over a long period. This is the first report on the use of genetic markers to establish genetic fidelity of long-term micropropagated banana using RAPD and ISSR.

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Section: Resultsmentioning

confidence: 99%

Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers

Lakshmanan¹,

Venkataramareddy²,

Neelwarne³

2007

Electron. J. Biotechnol.

150

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“…Though a very few exogenous growth regulators have been reported useful for banana micropropagation (Escalant and Teisson 1989;Bhagyalakshmi and Singh 1995;Dhed'a et al 1991), the sub-and supra-optimal levels of growth regulators, especially the synthetic ones, were found to induce somaclonal variation (Peres et al 1999;Smith 1988;Martins et al 2004;Martin et al 2006). Even in cases where immediate variations were not noticed, the long-term application of high levels of cytokinins resulted in somaclonal or epigenetic variations in some of the micropropagated plants (Israeli et al 1996;Damasco et al 1996) questioning the very fidelity of their clonal nature. This apart, banana shoots in vitro are known to synthesize and accumulate a natural cytokinin, 2iP at the basal portions of shoot clusters resulting in de novo bud formation, especially under an exogenous supply of BA (Zaffari et al 2000;Venkatachalam et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation in banana using high levels of cytokinins does not involve any genetic changes as revealed by RAPD and ISSR markers

2007

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Use of high levels of growth regulators during micropropagation results in undesirable clonal variability in important commercial crops such as banana. The present study investigated the effects of high levels of cytokinins on micropropagation in banana (genotype AAB), and the genetic stability of plantlets was assessed using RAPD and ISSR markers. Cytokinins, such as BA and kinetin were added to the routine shoot multiplication medium at concentrations up to 10 mg l -1 . After 12 weeks of culture involving three subcultures, the maximum number of shoot buds were produced in cultures receiving either 5 mg l -1 BA (80 shoot buds) or 4 mg l -1 kinetin (62 shoot buds). Certain morphological abnormalities observed during proliferation of shoot buds in vitro were not observed during acclimatization ex vitro. To check the genetic stability, RAPD and ISSR profiles of micropropagated plantlets obtained from different cytokinin-treatments were compared with control microplants maintained on MS medium as well as the field-grown mother plant. A total of 50 RAPD and 12 ISSR primers resulted in 625 distinct and reproducible bands. Thus a total of 17,400 bands were generated showing homogeneous RAPD and ISSR patterns. Band intensity histogram of each gel confirmed their monomorphic nature with no genetic variation in all the plantlets analysed. Based on these results a protocol for high rate shoot multiplication was worked out leading to uniform shoot production.

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“…Low stature or "Dwarf", frequently observed in somaclonal variants of banana trees (Israeli et al, 1996), is characterized by reduced plant size and unsatisfactory production. In this trial, in addition to low stature, super budding and general discoloration of the shoot were observed, and no phytopathogen or nutritional deficiency that could mark the results was diagnosed.…”

Section: Lsv (Low Stature Variant)mentioning

confidence: 99%

Somaclonal variation in micropropagated Heliconia bihai cv. Lobster Claw I plantlets (Heliconiaceae)

Rodrigues

2008

Sci. agric. (Piracicaba, Braz.)

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The occurrence of somaclonal variation is described in various cultures of agronomic interest. Such variation can be of benefit in the development of new flower varieties. In this study, the occurrence of somaclonal variation in micropropagated changes of Heliconia bihai cv. Lobster Claw I was investigated. Stem apexes were introduced in MS culture media with the addition of 2.5 mg L -1 of benzylaminopure (BAP) and 500 mg L -1 of sodium cefotaxime. After selecting the apex stem, it was subcultivated in MS media and supplemented with 4.0 mg L -1 of BAP to induce side buds. To conduct the trial, 2,000 plants were selected and compared with plants originated from rhizomes. To calculate the percentage of the variants, the plant stature, the form and color of leaves and pseudostem were evaluated. The plants with buds presenting the same type of variation were considered as variants. The occurrence of three types of somaclonal variants was observed: Variation of the Chlorophyll in the Leaf, Low Stature Variant and Pseudostem and Petiole Color Variant, the latter with ornamental potential. The somaclonal variation rate for Heliconia bihai cv Lobster Claw I, under the proposed conditions, was 61.40%.

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Selection of stable banana clones which do not produce dwarf somaclonal variants during in vitro culture

Cited by 29 publications

References 13 publications

Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers

Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers

Micropropagation in banana using high levels of cytokinins does not involve any genetic changes as revealed by RAPD and ISSR markers

Somaclonal variation in micropropagated Heliconia bihai cv. Lobster Claw I plantlets (Heliconiaceae)

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