Selection of potential probiotic Lactobacillus strains of human origin for use in dairy industry

Španová, Alena; Dráb, Vladimí­r; Turková, Kristýna; Špano, Miroslav; Burdychová, Radka; Šedo, Ondřej; Šrůtková, Dagmar; Rada, V.; Rittich, Bohuslav

doi:10.1007/s00217-015-2511-1

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…The MALDI-ToF MS technique was used as a pre-identification step because it has been considered a reliable method for LAB identification [12,38]. Additionally, MALDI-ToF MS technique enables a comparison among protein profile of bacterial isolates of interest with previously known protein profile of bacterial species to discard subsequent experiment with pre-identified pathogens [13,19], with a practical application to search for probiotic isolates [12,24].…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, MALDI-ToF MS technique enables a comparison among protein profile of bacterial isolates of interest with previously known protein profile of bacterial species to discard subsequent experiment with pre-identified pathogens [13,19], with a practical application to search for probiotic isolates [12,24]. Some studies in available literature have shown the performance and reproducibility of MALDI-ToF MS to identify LAB species from food sources [19,24,38,39], including fruit [12,40]. The application of MALDI-ToF MS profiling to LAB identification has relied on some advantages, such as high-throughput, robustness, recognizing-based method, fast tendency test, ease of use, and low cost per test [38,41], which could support phenotypic methods for fast identification in a first characterization step of LAB culturable community in foods [41].…”

Section: Discussionmentioning

confidence: 99%

“…Some studies in available literature have shown the performance and reproducibility of MALDI-ToF MS to identify LAB species from food sources [19,24,38,39], including fruit [12,40]. The application of MALDI-ToF MS profiling to LAB identification has relied on some advantages, such as high-throughput, robustness, recognizing-based method, fast tendency test, ease of use, and low cost per test [38,41], which could support phenotypic methods for fast identification in a first characterization step of LAB culturable community in foods [41]. However, MALDI-ToF MS profiling for bacterial identification could have some drawbacks, including a method with relative low analytical sensitivity (approximately 10 5 -10 6 bacteria/deposit) and a restrict content of available MS profile database [41,42], which could be associated with the number of isolates not reaching a secure identification level in MALDI-ToF MS profiling.…”

Section: Discussionmentioning

confidence: 99%

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Selection of lactic acid bacteria with promising probiotic aptitudes from fruit and ability to survive in different food matrices

2021

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Lactic acid bacteria (LAB) are among the most prevalent microorganisms forming the autochthonous microbiota of fruit. This study aimed to select LAB isolates with probiotic aptitudes from apple, banana, grape, and orange through evaluation of in vitro safety, technological, and functional-related properties. The ability of most promising selected isolates to survive in commercial apple and orange juice, meat stew, vegetable puree, and UHT milk during 28 days of refrigeration storage was evaluated. Ninety-three isolates identified preliminarily as LAB were recovered from fruit and 66 of these isolates passed safety tests. Most of these isolates were pre-identified as belonging to Lactobacillus or Enterococcus genus based on MALDI-ToF MS profiling. These 66 isolates were categorized into three homogeneous groups based on a preliminary cluster analysis run with data from experiments to measure technological characteristics. Nine LAB isolates were selected as the most promising for probiotic use based on a principal component analysis run with data from experiments to measure probioticrelated properties. Four of these isolates were sensitive to different antibiotics and identified (16S-rRNA gene sequencing) as Lactobacillus brevis (recently reclassified as L. brevis) or Lactobacillus spp. These 4 selected isolates had high viable counts and high percentages of physiologically active cells in apple and orange juice, beef stew, vegetable puree, and UHT milk during refrigeration storage. The results showed that apple, banana, orange, and grape are potential sources of LAB with aptitudes to be exploited for a possible probiotic use and distinguished abilities to survive in different food matrices.

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