Selection of peptides for serological detection of equine infectious anemia

Abstract

ABSTRACT. Equine infectious anemia caused by equine infectious anemia virus is an important disease due to its high severity and incidence in animals. We used a phage display library to isolate peptides that can be considered potential markers for equine infectious anemia diagnosis. We selected peptides using IgG purified from a pool comprised of 20 sera from animals naturally infected with equine infectious anemia virus. The diagnostic potential of these peptides was investigated by ELISA, Western blot and dot blot with purified IgG and serum samples. Based on the results, we chose a peptide mimetic for glycoprotein gp45 epitopes of equine infectious anemia virus, with potential for use as an antigen in indirect diagnostic assays. Synthesis of this peptide has possible applications for the development of new diagnostic tools for this disease.

A conformational epitope mapped in the bovine herpesvirus type 1 envelope glycoprotein B by phage display and the HSV-1 3D structure

“…The selection of peptides to mimic protein structure motifs based on the peptide library approach is a powerful tool for antigen prospecting across a wide range of microorganisms. However, the majority of studies that used this methodology are based on the primary structure of the peptides (Gazarian et al, 2011;Santos et al, 2012;Prudencio et al, 2011;Cunha-Junior et al, 2010). To address the lack of available information related to the 3D structure of BoHV-1 proteins, we demonstrated an alternative in silico approach for epitope mapping of BoHV-1 that was based on recombinant peptides selected by phage display and then conformationally aligned with the homologous 3D structure of Herpes Simplex Virus type 1 (HSV-1).…”

Evaluation of Equine Infectious Anemia Virus by the Indirect Enzyme-linked Immunosorbent Assay EIA-LAB as Screening Tools in Mexico

“…The ELISA AIE-LAB is an indirect heterogenic system designed and produced in Cuba with the purpose of enhancing the efficiency of the National Diagnostic Program of EIA. The ELISA EIA-LAB uses a synthetic peptide from gp45 protein as antigen for detection of EIAV antibodies presenting advantages over the use of natural antigens because they increase the sensitivity and eliminates crossed reactions [24]. The primary goal of this study was to evaluate the ELISA AIE-LAB performance in CIESA laboratory of UAEM University.…”

“…Previous studies have reported that antibodies against capsid p26 protein are produced after glycoproteins antibodies because gp45 is a transmembrane protein and gp90 an integral membrane protein, both more exposed from immune system recognition [30,31]. Despite the rapid and high rate of env gene mutations, as a viral mechanism for evasion of the immune system response, conserved regions in the env sequence have been identified for the diagnostic and production of immunogenic peptides [24,32,33].…”

Development, evaluation, and laboratory validation of immunoassays for the diagnosis of equine infectious anemia (EIA) using recombinant protein produced from a synthetic p26 gene of EIA virus

“…In an effort to develop a more rapid and sensitive diagnostic capability, several attempts have been made on development of enzyme-linked immunosorbent assays (ELISAs) based on p26 protein purified from EIAV strain [28,29,32], recombinant proteins [1,2,15,22,25,33] and peptides [27,31]. The EIAV p26 protein encoded by gag gene is one of the highly conserved structural proteins among the viral isolates even from very different geographical locations [4,5,26].…”