Selection of parental strain on the sawdust cultivation and mycelial growth and cultural characteristics of Lentinula edodes hybrid strains

Noh, Jong-Hyun; Ko, Han-Gyu; 박흥수,; Koo, Chang-Duck

doi:10.14480/jm.2015.13.1.41

Cited by 9 publications

(7 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Edible basidiomycetous mushrooms have been bred with selection breeding, hybrid breeding by mating, biotechnological breeding using tissue culturing and protoplast fusion, and mutation breeding using radiation or chemical substances [9]. For the hybrid breeding methods, well-characterized genetic characteristics of parental strains, such as productivity, fruiting body traits, fruiting temperature, and developmental patterns, are mainly targeted [10]. Chamaram (strain # 07–84), a L. edodes strain, was developed as a suitable strain grown on sawdust media by the hybrid breeding method in South Korea.…”

Section: Introductionmentioning

confidence: 99%

“…Chamaram (strain # 07–84), a L. edodes strain, was developed as a suitable strain grown on sawdust media by the hybrid breeding method in South Korea. This strain displays a high productivity, with approximately 278.5 g of fruiting body per 1.3 kg in sawdust media and excellent fruiting body shapes [10]. With these attractive properties, the Chamaram strain is one of the most popular cultivated strains in south Korea.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative transcriptome analysis identified candidate genes involved in mycelium browning in Lentinula edodes

et al. 2019

View full text Add to dashboard Cite

BackgroundLentinula edodes is one of the most popular edible mushroom species in the world and contains useful medicinal components, such as lentinan. The light-induced formation of brown film on the vegetative mycelial tissues of L. edodes is an important process for ensuring the quantity and quality of this edible mushroom. To understand the molecular mechanisms underlying this critical developmental process in L. edodes, we characterized the morphological phenotypic changes in a strain, Chamaram, associated with abnormal brown film formation and compared its genome-wide transcriptional features.ResultsIn the present study, we performed genome-wide transcriptome analyses of different vegetative mycelium growth phenotypes, namely, early white, normal brown, and defective dark yellow partial brown films phenotypes which were exposed to different light conditions. The analysis revealed the identification of clusters of genes specific to the light-induced brown film phenotypes. These genes were significantly associated with light sensing via photoreceptors such as FMN- and FAD-bindings, signal transduction by kinases and GPCRs, melanogenesis via activation of tyrosinases, and cell wall degradation by glucanases, chitinases, and laccases, which suggests these processes are involved in the formation of mycelial browning in L. edodes. Interestingly, hydrophobin genes such as SC1 and SC3 exhibited divergent expression levels in the normal and abnormal brown mycelial films, indicating the ability of these genes to act in fruiting body initiation and formation of dikaryotic mycelia. Furthermore, we identified the up-regulation of glycoside hydrolase domain-containing genes in the normal brown film but not in the abnormal film phenotype, suggesting that cell wall degradation in the normal brown film phenotype is crucial in the developmental processes related to the initiation and formation of fruiting bodies.ConclusionsThis study systematically analysed the expression patterns of light-induced browning-related genes in L. edodes. Our findings provide information for further investigations of browning formation mechanisms in L. edodes and a foundation for future L. edodes breeding.Electronic supplementary materialThe online version of this article (10.1186/s12864-019-5509-4) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis identified candidate genes involved in mycelium browning in Lentinula edodes

et al. 2019

View full text Add to dashboard Cite

show abstract

“…To identify whether the abnormal mycelial film phenotype was genetically determined, the trait was examined in the pedigree of the Chamaram cultivar ( Figure 1 c). Chamaram is a hybrid line derived from the mating of monokaryotic P37-5 and SJ701-51 strains ( Figure 1 c; [ 18 ]). To test whether the abnormal browning phenotype was determined by homozygotic or heterozygotic alleles, dikaryotic isolates were generated by the self-mating of compatible monokaryotic spores collected from a Chamaram fruiting body, and their mycelial film phenotypes were assessed.…”

Section: Resultsmentioning

confidence: 99%

Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes

Hong

Moon

Yoo

et al. 2020

JoF

Self Cite

View full text Add to dashboard Cite

Lentinula edodes is a globally important edible mushroom species that is appreciated for its medicinal properties as well as its nutritional value. During commercial cultivation, a mycelial brown film forms on the surface of the sawdust growth medium at the late vegetative stage. Mycelial film formation is a critical developmental process that contributes to the quantity and quality of the mushroom yield. However, little is known regarding the genetic underpinnings of brown film formation on the surface of mycelial tissue. A novel causal gene associated with the formation of the mycelial brown film, named ABL (Abnormal browning related to light), was identified in this study. The comparative genetic analysis by dihybrid crosses between normal and abnormal browning film cultivars demonstrated that a single dominant allele was responsible for the abnormal mycelium browning phenotype. Whole-genome sequencing analysis of hybrid isolates revealed five missense single-nucleotide polymorphisms (SNPs) in the ABL locus of individuals forming abnormal partial brown films. Additional whole-genome resequencing of a further 16 cultivars showed that three of the five missense SNPs were strongly associated with the abnormal browning phenotype. Overexpression of the dominant abl-D allele in a wild-type background conferred the abnormal mycelial browning phenotype upon transformants, with slender hyphae observed as a general defective mycelial growth phenotype. Our methodology will aid the future discovery of candidate genes associated with favorable traits in edible mushrooms. The discovery of a novel gene, ABL, associated with mycelial film formation will facilitate marker-associated breeding in L. edodes.

show abstract

“…Three shiitake strains of Sanjo 701ho, Youjiro, and Chamaram cultivars were maintained on PDA at 25 C for 15 days. Cultivation of these mushrooms was undertaken on the oak sawdust substrate as described by Noh et al [17].…”

Section: Fungal Strains and Mushroommentioning

confidence: 99%

Pathological Properties of Cryptococcus pseudolongus on the Mycelia and Fruit Body of Lentinula edodes

Kwon

Kim

2021

Mycobiology

View full text Add to dashboard Cite

Recently, Cryptococcus pseudolongus has been reported as a new pathogen of shiitake (Lentinula edodes). However, its pathological properties are not much known. To further understand its impact on the mushroom, we investigated the pathogen's interactions with the mycelium of shiitake, histopathological properties, host range, and sensitivity to diverse antifungal agents. The strain C. pseudolongus DUCC 4014 inhibited the mycelial growth of L. edodes strain (cultivar Sanjo 701ho) and caused browning in the mycelia confronted with the yeast on PDA. Spray inoculation of the yeast caused an abnormal browning symptom on the cap and/or gills of three shiitake cultivars grown on sawdust media in vinyl bags. Scanning electron microscopic images of the abnormally browned parts of shiitake fruit body illustrated that mushroom tissues were loosed and dispersed in the middle and edge of the cap and the arrangement of basidiospores borne on basidia in the gills was disturbed compared to those of normal shiitake fruit body. Spray inoculation also led to developing abnormal browning on the harvested fruit body, indicating C. pseudolongus could be a problem during mushroom storage. But the yeast was not able to induce abnormal browning on mushrooms of Pleurotus ferulae, Pleurotus fostreatus, and Agaricus bisporus. But it induced browning only on button mushroom (A. bisporus) when they were inoculated after wounding. Tests with 16 kinds of fungicides revealed that the cell growth of C. pseudolongus could be inhibited by benzalkonium chloride at MIC 7 lg/ml and benomyl at MIC 3 lg/ml.

show abstract

Selection of parental strain on the sawdust cultivation and mycelial growth and cultural characteristics of Lentinula edodes hybrid strains

Cited by 9 publications

References 6 publications

Comparative transcriptome analysis identified candidate genes involved in mycelium browning in Lentinula edodes

Comparative transcriptome analysis identified candidate genes involved in mycelium browning in Lentinula edodes

Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes

Pathological Properties of Cryptococcus pseudolongus on the Mycelia and Fruit Body of Lentinula edodes

Contact Info

Product

Resources

About