Selection of oleaginous yeasts with high lipid productivity for practical biodiesel production

Tanimura, Ayumi; Takashima, Masako; Sugita, Takashi; Endoh, Rikiya; Kikukawa, Minako; Yamaguchi, S.; Sakuradani, Eiji; Ogawa, Jun; Shima, Jun

doi:10.1016/j.biortech.2013.11.086

Cited by 86 publications

(47 citation statements)

References 25 publications

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“…Over 40 oleaginous yeast species are known, but the list is lengthening since TAGs-producers have been increasingly searched among environmental yeast isolates, the metabolic potential of which being still understudied. For most of them, however, the ability to accumulate lipids has been assessed in glucose-based media [2,25], without an exhaustive survey on the oleaginicity on glycerol.…”

Section: Resultsmentioning

confidence: 99%

Getting lipids from glycerol: new perspectives on biotechnological exploitation of Candida freyschussii

et al. 2014

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BackgroundMicrobial lipids represent a valuable alternative feedstock for biodiesel production when oleaginous microbes are cultured with inexpensive substrates in processes exhibiting high yield and productivity. In this perspective, crude glycerol is among the most promising raw materials for lipid production, because it is the costless residual of biodiesel production. Thus, cultivation of oleaginous yeasts in glycerol-based media is attracting great interest and natural biodiversity is increasingly explored to identify novel oleaginous species recycling this carbon source for growth and lipid production.ResultsThirty-three yeasts strains belonging to 19 species were screened for the ability to grow and produce intracellular lipids in a pure glycerol-based medium with high C/N ratio. A minority of them consumed most of the glycerol and generated visible lipid bodies. Among them, Candida freyschussii ATCC 18737 was selected, because it exhibited the highest lipid production and glycerol conversion yield. Lipid production in this strain was positively affected by the increase of C/N ratio, but growth was inhibited by glycerol concentration higher than 40 g/L. In batch cultures, the highest lipid production (4.6 g/L), lipid content of biomass (33% w/w), and lipid volumetric productivity (0.15 g/L/h) were obtained with 40 g/L glycerol, during the course of a 30-h process. Fed-batch cultivation succeeded in preventing substrate inhibition and in achieving a high cell-density culture. The improved lipid production and volumetric productivity reached the remarkable high level of 28 g/L and 0.28 g/L/h, respectively. The lipids accumulated by C. freyschussii ATCC 18737 have similar fatty acid composition of plant oil indicating their potential use as biodiesel feedstock. Calculated physicochemical properties of a biodiesel produced with the lipids from C. freyschussii ATCC 18737 are expected to meet the European and American standards, being equal to those of rapeseed and palm biodiesel.ConclusionsC. freyschussii ATCC 18737 could be considered an interesting microorganism for utilization in biofuel industry. Cultivation of this yeast in media containing crude glycerol should be investigated deeper in order to evaluate whether it may find application in the valorization of the waste of biodiesel manufacturing.

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Section: Resultsmentioning

confidence: 99%

Getting lipids from glycerol: new perspectives on biotechnological exploitation of Candida freyschussii

et al. 2014

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“…Lipids bound to the tri-functional microparticles were recovered by dissolving them in hexane and subsequent acid catalyzed transesterification and gas chromatographic analysis [27]. Based on the fatty acid composition, key biodiesel properties such as viscosity, specific gravity, cloud point, cetane number, iodine number, and higher heating value (HHV) were estimated [28].…”

Section: Microalgal Harvesting Testmentioning

confidence: 99%

Tri-functionality of Fe3O4-embedded carbon microparticles in microalgae harvesting

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Praveenkumar

et al. 2015

Chemical Engineering Journal

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“…The ratio of the sugars and their concentration in the hydrolysates vary depending on the feedstock used and pretreatment conditions (Behera et al 2014; Kumar et al 2009). A previous study investigated lipid accumulation using a medium containing 3 % glucose by Vanrija musci JCM 24512 (formally Cryptococcus musci ) (Tanimura et al 2014b). The strain showed higher lipid-producing ability from glucose compared to typical oleaginous yeasts such as Lipomyces starkeyi and Rhodosporidium toruloides .…”

Section: Introductionmentioning

confidence: 99%

Lipid production through simultaneous utilization of glucose, xylose, and l-arabinose by Pseudozyma hubeiensis: a comparative screening study

et al. 2016

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Co-fermentation of glucose, xylose and l-arabinose from lignocellulosic biomass by an oleaginous yeast is anticipated as a method for biodiesel production. However, most yeasts ferment glucose first before consuming pentoses, due to glucose repression. This preferential utilization results in delayed fermentation time and lower productivity. Therefore, co-fermentation of lignocellulosic sugars could achieve cost-effective conversion of lignocellulosic biomass to microbial lipid. Comprehensive screening of oleaginous yeasts capable of simultaneously utilizing glucose, xylose, and l-arabinose was performed by measuring the concentration of sugars remaining in the medium and of lipids accumulated in the cells. We found that of 1189 strains tested, 12 had the ability to co-ferment the sugars. The basidiomycete yeast Pseudozyma hubeiensis IPM1-10, which had the highest sugars consumption rate of 94.1 %, was selected by culturing in a batch culture with the mixed-sugar medium. The strain showed (1) simultaneous utilization of all three sugars, and (2) high lipid-accumulating ability. This study suggests that P. hubeiensis IPM1-10 is a promising candidate for second-generation biodiesel production from hydrolysate of lignocellulosic biomass.

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Selection of oleaginous yeasts with high lipid productivity for practical biodiesel production

Cited by 86 publications

References 25 publications

Getting lipids from glycerol: new perspectives on biotechnological exploitation of Candida freyschussii

Getting lipids from glycerol: new perspectives on biotechnological exploitation of Candida freyschussii

Tri-functionality of Fe3O4-embedded carbon microparticles in microalgae harvesting

Lipid production through simultaneous utilization of glucose, xylose, and l-arabinose by Pseudozyma hubeiensis: a comparative screening study

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