Lipid production through simultaneous utilization of glucose, xylose, and l-arabinose by Pseudozyma hubeiensis: a comparative screening study

Tanimura, Ayumi; Takashima, Masako; Sugita, Takashi; Endoh, Rikiya; Ohkuma, Moriya; Kishino, Shigenobu; Ogawa, Jun; Shima, Jun

doi:10.1186/s13568-016-0236-6

Cited by 36 publications

(14 citation statements)

References 40 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, some strains can consume more than two sugars simultaneously. For instance, strains of Rhodosporidium and Pseudozyma show a simultaneous consumption of glucose, xylose and fructose [115] and glucose, xylose and arabinose, respectively [116]. Likewise, Rhodosporidium kratochvilovae and Y. lipolytica showed a simultaneous consumption of different pentoses and hexoses on mixed sugar media [117,118].…”

Section: Sugar Conversion Into Sco and Regulatory Mechanisms In Sugarmentioning

confidence: 99%

Lignocellulosic Biomass as a Substrate for Oleaginous Microorganisms: A Review

Valdés¹,

Mendonça²,

Aggelis³

2020

Preprint

View full text Add to dashboard Cite

The microorganisms able of accumulating lipids in high percentages, known as oleaginous microorganisms, have been widely studied as an alternative for producing oleochemicals and biofuels. Microbial lipid, so called Single Cell Oil (SCO), production depends on several growth parameters, including the nature of the carbon substrate, which must be efficiently taken up and converted into storage lipid. Οn the other hand, substrates considered for large scale applications must be abundant and of low acquisition cost. Among others, lignocellulosic biomass is a promising renewable substrate containing high percentages of assimilable sugars (hexoses and pentoses). However, it is also highly recalcitrant and therefore it requires specific pretreatments in order to release its assimilable components. The main drawback of lignocellulose pretreatment is the generation of several by-products that can inhibit the microbial metabolism. In this review, we discuss the main aspects related to the cultivation of oleaginous microorganisms using lignocellulosic biomass as substrate, hoping to contribute to the development of a sustainable process for SCO production in the near future.

show abstract

Section: Sugar Conversion Into Sco and Regulatory Mechanisms In Sugarmentioning

confidence: 99%

Lignocellulosic Biomass as a Substrate for Oleaginous Microorganisms: A Review

Valdés¹,

Mendonça²,

Aggelis³

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Simultaneous mixed-sugar utilization was achieved, which was attributed to mild glucose repression in conjunction with strong carbon catabolite activation for growth on xylose and cellobiose. In another exhaustive screening of 1,189 yeast isolates, Tanimura et al demonstrated P. hubeiensis IPM1-10 was able to co-utilize glucose, xylose, and arabinose in the artificial hydrolysate and accumulated high amounts of lipid (Tanimura et al, 2016). These findings suggest the importance of broadening the current collection of microbial species for efficient fermentation of multiple sugars in biomass hydrolysates.…”

Section: Native Xylose-utilizing Yeastsmentioning

confidence: 99%

Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts

2019

View full text Add to dashboard Cite

Plant biomass is a promising carbon source for producing value-added chemicals, including transportation biofuels, polymer precursors, and various additives. Most engineered microbial hosts and a select group of wild-type species can metabolize mixed sugars including oligosaccharides, hexoses, and pentoses that are hydrolyzed from plant biomass. However, most of these microorganisms consume glucose preferentially to non-glucose sugars through mechanisms generally defined as carbon catabolite repression. The current lack of simultaneous mixed-sugar utilization limits achievable titers, yields, and productivities. Therefore, the development of microbial platforms capable of fermenting mixed sugars simultaneously from biomass hydrolysates is essential for economical industry-scale production, particularly for compounds with marginal profits. This review aims to summarize recent discoveries and breakthroughs in the engineering of yeast cell factories for improved mixed-sugar co-utilization based on various metabolic engineering approaches. Emphasis is placed on enhanced non-glucose utilization, discovery of novel sugar transporters free from glucose repression, native xylose-utilizing microbes, consolidated bioprocessing (CBP), improved cellulase secretion, and creation of microbial consortia for improving mixed-sugar utilization. Perspectives on the future development of biorenewables industry are provided in the end.

show abstract

“…[26][27][28] Exhaustive screening of large number of yeast isolates led to the discovery of Pseudozyma hubeiensis IPM1-10 that converts sugar mixtures consisting of glucose, xylose and arabinose to lipids. 29 Oleaginous microbes utilise xylose exclusively by phosphoketolase pathway resulting in lower yields of lipids. 30 Due to the absence of glucose repression in P. hubeiensis, this yeast will turn out to be a potential candidate for second generation biodiesel production from hydrolysate of cellulosic biomass.…”

Section: Enzymes and Lipids Produced By Pseudozyma Hubeiensismentioning

confidence: 99%

Pseudozyma hubeiensis, an unexplored yeast: It’s potential in biomass conversion to value added products

Mhetras

Gokhale

2018

JBMOA

View full text Add to dashboard Cite

The genus pseudozyma The species of the genus Pseudozyma belong to ustilaginales based on the morphological studies 1 and molecular characterization. 2,3 There are 11 species reported so far from this genus that are distinguished by analysing the sequences of combined ITS and D1/D2 regions. Boekhout & Fell 4 reported seven species and Sugita et al. 5 described two species isolated from blood of the patients. Two more species of Pseudozyma were isolated from wilting leaves of different plants that are named as Pseudozyma hubeiensis and Psedozyma shanxiensis. 6 These species are distinguished from other reported species by morphological studies and physiological characterization. The novelty of these two species was also confirmed by molecular taxonomic analysis based on sequencing of 26S rRNA gene, D1/ D2 domain and internal transcribed spacer (ITS) regions (6). All 11 species of Pseudozyma were further studied for assimilation reactions which differentiated all the species of the genus Pseudozyma from one another. The ability to grow at 40°C and no assimilation of erythritol are the characteristics that differentiate P. shanxiensis from all other species of Pseudozyma. However, P. hubeiensis does not assimilate inositol and this characteristic differentiates P. hubeiensis from all other species. 6 P. hubeiensis was first isolated from decaying sandal wood in our laboratory 7 and then it was sent for identification to National Collection of Yeast Cultures (NCYC) in 2008. The sequencing of 26 rDNA D1/D2 domain and standard taxonomic tests confirmed that it is a novel strain of P. hubeiensis. It was then deposited in NCIM Resource Center, CSIR-National Chemical Laboratory, Pune, with an accession number NCIM 3574. Among all species of Pseudozyma, Pseudzyma antarctica (Formally known as Candida antarctica) has been a most studied. It is well known producer of glycolipid, manosylerythritol (MEL) from vegetable oil including soybean oil, alkanes, glycerol, glucose and xylose 8 and also from cellulosic materials. 9 MELs show excellent surface active properties in addition to versatile biochemical actions. In addition to its bio-surfactant property, MEL possesses antitumor and cell differentiation induction activities. 10 The other species such as P. aphidis, P. rugulosa, P. fusiformata are also known to produce MEL. 11 Extracellular esterases 12 and biodegradable plastic degrading enzymes 13 have been reported from P. antarctica. The plastic degrading enzyme named PaE produced by P. antarctica degrades biodegradable plastic films composed of poly (butylele succinate) (PBS), poly (butylene succinate-co-adipate) (PBSA) and poly (lactic acid) (PLA). Xylose induced xylanases were reported from P. antarctica and the 33 kDa purified protein was found to produce xylose from xylan indicating that they are endo-xylanases. 14 Genome and transcriptome analysis of P. antarctica was recently reported. 15 The another novel yeast species, P. brasiliensis produced the xylan induced secretome containing endo-xylanase and β-xylosidase. 16

show abstract

Lipid production through simultaneous utilization of glucose, xylose, and l-arabinose by Pseudozyma hubeiensis: a comparative screening study

Cited by 36 publications

References 40 publications

Lignocellulosic Biomass as a Substrate for Oleaginous Microorganisms: A Review

Lignocellulosic Biomass as a Substrate for Oleaginous Microorganisms: A Review

Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts

Pseudozyma hubeiensis, an unexplored yeast: It’s potential in biomass conversion to value added products

Contact Info

Product

Resources

About