Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts

Gao, Min; Ploessl, Deon; Shao, Zengyi

doi:10.3389/fmicb.2018.03264

Cited by 52 publications

(40 citation statements)

References 192 publications

(277 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the relatively slow consumption of other sugars after depletion of glucose (Jin, Laplaza, & Jeffries, 2004) significantly decreases the overall sugar consumption rate and limits achievable productivity in the bioprocess. Overcoming this delayed and slow consumption of mixed sugars is essential for the development of an efficient and economic bioprocess, particularly for the development of a consolidated bioprocess (Brethauer & Studer, 2014;Gao, Ploessl, & Shao, 2018).…”

Section: Introductionmentioning

confidence: 99%

Engineering of Klebsiella oxytoca for production of 2,3‐butanediol using mixed sugars derived from lignocellulosic hydrolysates

et al. 2020

View full text Add to dashboard Cite

2,3‐Butanediol (2,3‐BDO) is a promising bulk chemical owing to its high potential in industrial applications. Here, we engineered Klebsiella oxytoca for the economic production of 2,3‐BDO using mixed sugars from renewable biomass. First, to improve xylose consumption, the xylose transporter gene (xylE) was integrated into the methylglyoxal synthase A (mgsA)‐coding gene loci, and the engineered CHA004 strain showed much faster consumption of xylose than wild‐type (WT) strain with 1.4‐fold increase of overall sugar consumption rate. To further improve sugar utilization, we performed adaptive laboratory evolution for 90 days. The evolved strain (CHA006) was evaluated by cultivating it in the media containing single‐ or mixed‐sugars, and it was clearly observed that CHA006 has improved sugar consumption and 2,3‐BDO production than those of the parental strain. Finally, we demonstrated the superiority of CHA006 by culturing in two lignocellulosic hydrolysates derived from sunflower or pine tree. Particularly, in the pine tree hydrolysate containing xylose, glucose, galactose, and mannose, the CHA006 strain showed much improved consumption rates for all sugars, and 2,3‐BDO productivity (0.73 g L−1 hr−1) increased by 3.2‐fold compared to WT strain. We believe that the engineered CHA006 strain can be a potential host in the development of economic bioprocess for 2,3‐BDO through efficient utilization of mixed sugars derived from lignocellulosic biomass.

show abstract

Section: Introductionmentioning

confidence: 99%

Engineering of Klebsiella oxytoca for production of 2,3‐butanediol using mixed sugars derived from lignocellulosic hydrolysates

et al. 2020

View full text Add to dashboard Cite

show abstract

“…xylose is rarely present in the environment and in free form in natural products. If the pathways exist, it is not a preferred carbon source and its uptake is suppressed in the presence of glucose due to carbon catabolite repression [7]. These challenges make pentose sugar a lesser lucrative option as an exploitable carbon source.…”

Section: Introductionmentioning

confidence: 99%

Biovalorisation of crude glycerol and xylose into xylitol by oleaginous yeast Yarrowia lipolytica

Prabhu

Thomas

Amaro

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Xylitol is a commercially important chemical with multiple applications in the food and pharmaceutical industries. According to the US Department of Energy, xylitol is one of the top twelve platform chemicals that can be produced from biomass. The chemical method for xylitol synthesis is however expensive and energy intensive. In contrast, the biological route using microbial cell factories offers a potential cost-effective alternative process. The bioprocess occurs under ambient conditions and makes use of biocatalysts and biomass which can be sourced from renewable carbon originating from a variety of cheap waste feedstocks. Result: In this study, biotransformation of xylose to xylitol was investigated using Yarrowia lipolytica an oleaginous yeast which was firstly grown on a glycerol/glucose for screening of co-substrate, followed by media optimisation in shake flask, scale up in bioreactor and downstream processing of xylitol. A two-step medium optimization was employed using central composite design and artificial neural network coupled with genetic algorithm. The yeast amassed a concentration of 53.2 g/L xylitol using pure glycerol (PG) and xylose with a bioconversion yield of 0.97 g/g. Similar results were obtained when PG was substituted with crude glycerol (CG) from the biodiesel industry (titer: 50.5 g/L; yield: 0.92 g/g). Even when xylose from sugarcane bagasse hydrolysate was used as opposed to pure xylose, a xylitol yield of 0.54 g/g was achieved. Xylitol was successfully crystallized from PG/xylose and CG /xylose fermentation broths with a recovery of 39.5 and 35.3%, respectively. Conclusion: To the best of the author’s knowledge, this study demonstrates for the first time the potential of using Y. lipolytica as a microbial cell factory for xylitol synthesis from inexpensive feedstocks. The results obtained are competitive with other xylitol producing organisms.

show abstract

“…The use of lignocellulosic raw material points to an opportunity to add value to agricultural waste, which does not compete against food supplies [3]. The lignocellulosic material is capable of providing sugars, especially D-glucose, D-xylose, and L-arabinose [3,4] that can be used to produce not only ethanol but also other valuable products, such as xylitol, arabitol, and carboxylic acids [5].…”

Section: Introductionmentioning

confidence: 99%

“…The global production of lignocellulosic biomass is estimated to be approximately 3-5 gigatons per year [6,7]. However, from the large contingent of hemicelluloses, little is used in fermentative processes, mostly due to the inability of wild-type Saccharomyces cerevisiae to efficiently co-utilize hexoses and pentoses [5,8].…”

Section: Introductionmentioning

confidence: 99%

“…In this context, prospecting transporters from organisms capable of metabolizing C 5 sugar has been the main strategy. By prospecting pentose transporters, some D-xylose-fermenting yeasts, such as Candida shehatae, Pichia stipitis, and Pachysolen tannophilus [3], have been well studied; however, the results of engineering competent co-transport in S. cerevisiae have been unsatisfactory [2,5,8,9].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prospecting for l-arabinose/d-xylose symporters from Pichia guilliermondii and Aureobasidium leucospermi

et al. 2019

View full text Add to dashboard Cite

With the strong trend toward sustainable technologies, such as the gradual substitution of fossil fuel consumption, improvement in the utilization of sugars from lignocellulosic biomass appears to be an alternative for bioenergy. However, from a number of C 5 sugars, few are used in fermentative processes for ethanol production. One of the reasons is because wild-type Saccharomyces cerevisiae is unable to efficiently co-utilize hexoses and pentoses via specific transporters for each type of sugar. Thus, a system of pentose uptake that is not modulated by D-glucose is required. Here, we were able to identify the presence of sugar/H + symporters for D-xylose and L-arabinose, especially for Pichia guilliermondii, where an uptake of D-glucose via symporter was not detected. The best D-xylose uptake route in P. guilliermondii exhibited a K M of 48 mM and V MAX of 0.48 mmol h −1 g −1 at the early stationary phase (24 h). For L-arabinose, the best route of uptake exhibited a K M of 109 mM and V MAX of 0.8 mmol h −1 g −1 on log phase (12 h). The highest kinetic uptake was observed when the final pH of the medium was below 7. In general, an alkaline medium limited the expression of symporters. The results obtained in this study will help in the further investigation of these symporters through their overexpression in engineered S. cerevisiae.

show abstract

Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts

Cited by 52 publications

References 192 publications

Engineering of Klebsiella oxytoca for production of 2,3‐butanediol using mixed sugars derived from lignocellulosic hydrolysates

Engineering of Klebsiella oxytoca for production of 2,3‐butanediol using mixed sugars derived from lignocellulosic hydrolysates

Biovalorisation of crude glycerol and xylose into xylitol by oleaginous yeast Yarrowia lipolytica

Prospecting for l-arabinose/d-xylose symporters from Pichia guilliermondii and Aureobasidium leucospermi

Contact Info

Product

Resources

About