Selection of oleaginous yeasts capable of high lipid accumulation during challenges from inhibitory chemical compounds

Juanssilfero, Ario Betha; Amza, Rezky Lastinov; Miyamoto, Nao; Otsuka, Hiromi; Matsumoto, Hana; Kihira, Chie; Thontowi, Ahmad; Yopi, -; Kahar, Prihardi; Prasetya, Bambang; Kondo, Akihiko

doi:10.1016/j.bej.2018.05.024

Cited by 28 publications

(13 citation statements)

References 39 publications

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“…3 ). Native oleaginous yeasts capable of co-metabolising glucose and xylose, the major carbon sources in lignocellulosic hydrolysates, include L. starkeyi [ 110 , 111 ], C. oleaginosus [ 96 ], Pseudozyma hubeiensis [ 95 ], M. pulcherrima [ 112 ] , Trichosporon cutaneum [ 94 ] and Trichosporon coremiiforme [ 113 ]. Partially hydrolysed media, used to reduce process complexity, cost or inhibitor formation, typically contain some polysaccharides [ 104 , 114 ], wherefore oleaginous yeast with oligosaccharide degrading capacity, such as C. oleaginosus [ 115 ] or M. pulcherrima [ 104 , 116 , 117 ], have been used.…”

Section: Feedstocks For Oleaginous Yeastsmentioning

confidence: 99%

“…Lipomyces starkeyi is one of the few prominent oleaginous yeasts which have kept their name since discovery in 1946 [ 18 ]. Of the family Lipomycetaceae, which are strong lipid producers, L. starkeyi is regarded as the species with highest “biotechnological value” with highest attainable lipid content and inhibitor tolerance [ 110 , 148 ]. The most used strains of this species are AS 2.1560 (China General Microbiological Culture Collection Center), DSM 70296 (German Collection of Microorganisms and Cell Cultures) and CBS 1807 (Central Bureau of Fungal Cultures) (Additional file 1 : Fig.…”

Section: Oleaginous Yeast Speciesmentioning

confidence: 99%

“…4 ). Strains of L. starkeyi have been shown to simultaneously ferment xylose in combination with glucose [ 110 ], cellobiose [ 109 ] or acetate [ 119 ], but not arabinose [ 111 ]. Remarkably, co-utilisation of carbon sources is rare amongst other oleaginous yeasts [ 195 ].…”

Section: Oleaginous Yeast Speciesmentioning

confidence: 99%

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The history, state of the art and future prospects for oleaginous yeast research

Abeln

Chuck

2021

Microb Cell Fact

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Lipid-based biofuels, such as biodiesel and hydroprocessed esters, are a central part of the global initiative to reduce the environmental impact of the transport sector. The vast majority of production is currently from first-generation feedstocks, such as rapeseed oil, and waste cooking oils. However, the increased exploitation of soybean oil and palm oil has led to vast deforestation, smog emissions and heavily impacted on biodiversity in tropical regions. One promising alternative, potentially capable of meeting future demand sustainably, are oleaginous yeasts. Despite being known about for 143 years, there has been an increasing effort in the last decade to develop a viable industrial system, with currently around 100 research papers published annually. In the academic literature, approximately 160 native yeasts have been reported to produce over 20% of their dry weight in a glyceride-rich oil. The most intensively studied oleaginous yeast have been Cutaneotrichosporon oleaginosus (20% of publications), Rhodotorula toruloides (19%) and Yarrowia lipolytica (19%). Oleaginous yeasts have been primarily grown on single saccharides (60%), hydrolysates (26%) or glycerol (19%), and mainly on the mL scale (66%). Process development and genetic modification (7%) have been applied to alter yeast performance and the lipids, towards the production of biofuels (77%), food/supplements (24%), oleochemicals (19%) or animal feed (3%). Despite over a century of research and the recent application of advanced genetic engineering techniques, the industrial production of an economically viable commodity oil substitute remains elusive. This is mainly due to the estimated high production cost, however, over the course of the twenty-first century where climate change will drastically change global food supply networks and direct governmental action will likely be levied at more destructive crops, yeast lipids offer a flexible platform for localised, sustainable lipid production. Based on data from the large majority of oleaginous yeast academic publications, this review is a guide through the history of oleaginous yeast research, an assessment of the best growth and lipid production achieved to date, the various strategies employed towards industrial production and importantly, a critical discussion about what needs to be built on this huge body of work to make producing a yeast-derived, more sustainable, glyceride oil a commercial reality.

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Section: Feedstocks For Oleaginous Yeastsmentioning

confidence: 99%

Section: Oleaginous Yeast Speciesmentioning

confidence: 99%

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The history, state of the art and future prospects for oleaginous yeast research

Abeln

Chuck

2021

Microb Cell Fact

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“…In many cases, the substrate needs pretreatment in order to release reducing sugars, shortchain organic acids or alcohols, which can be directly used by the microorganism [2,15]. The conversion of the raw material into the final carbon source often implies the production of biomass degradation side-products that could inhibit microbial growth during fermentation [25]. The specific process to achieve the final carbon source from each agro-industrial waste is very important since it not only affects the medium composition, the yeast growth, the lipid yield and productivity and the lipid profile of SCOs but, above all, the overall process costs.…”

Section: Applications Of C Oleaginosus In the Bioconversion Of Agro-industrial Wastes To Single-cell Oilsmentioning

confidence: 99%

“…For example, the use of sugar-containing hydrolysates from lignocellulosic crops is one the most common approaches [3,4,7,23,24]. However, the quality of biomass hydrolysates is essential for good production of TAGs, as the presence of furanic compounds deriving from the degradation of sugars, such as furfural and 5-hydroxymethylfurfural, can significantly alter yeast growth and oil yield [25,26]. In fact, some organic compounds, such as furaldehyde, formaldehyde, phenols, aliphatic acids, vanillic acid, uronic acid, 4-hydroxybenzoic acid, acetic acid and cinnamaldehyde, can act as inhibitors for different metabolic activities of oleaginous yeasts [27,28].…”

Section: Introductionmentioning

confidence: 99%

Cutaneotrichosporon oleaginosus: A Versatile Whole-Cell Biocatalyst for the Production of Single-Cell Oil from Agro-Industrial Wastes

et al. 2021

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Cutaneotrichosporon oleaginosus is an oleaginous yeast with several favourable qualities: It is fast growing, accumulates high amounts of lipids and has a very broad substrate spectrum. Its resistance to hydrolysis by-products makes it a promising biocatalyst for custom tailored microbial oils. C. oleaginosus can accumulate up to 60 wt.% of its biomass as lipids. This species is able to grow by using several compounds as a substrate, such as acetic acid, biodiesel-derived glycerol, N-acetylglucosamine, lignocellulosic hydrolysates, wastepaper and other agro-industrial wastes. This review is focused on state-of-the-art innovative and sustainable biorefinery schemes involving this promising yeast and second- and third-generation biomasses. Moreover, this review offers a comprehensive and updated summary of process strategies, biomass pretreatments and fermentation conditions for enhancing lipid production by C. oleaginosus as a whole-cell biocatalyst. Finally, an overview of the main industrial applications of single-cell oil is reported together with future perspectives.

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Strategies for Obtaining Biofuels Through the Fermentation ofC5‐Raw Materials: Part 2

et al. 2021

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Selection of oleaginous yeasts capable of high lipid accumulation during challenges from inhibitory chemical compounds

Cited by 28 publications

References 39 publications

The history, state of the art and future prospects for oleaginous yeast research

The history, state of the art and future prospects for oleaginous yeast research

Cutaneotrichosporon oleaginosus: A Versatile Whole-Cell Biocatalyst for the Production of Single-Cell Oil from Agro-Industrial Wastes

Strategies for Obtaining Biofuels Through the Fermentation ofC5‐Raw Materials: Part 2

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