Selection of microorganisms for biosurfactant production using agroindustrial wastes

Abstract

Biosurfactant production by some bacterial isolates using molasses, milk whey and cassava flour wastewater (manipueira) as substrates was evaluated and compared with the production in conventional medium. Isolates growing in manipueira medium decreased the surface tension around 42%, the highest reduction among all the substrates tested. From the eleven isolates tested, eight were able to decrease the surface tension to levels below 30 mN/m using manipueira as substrate. The isolates LB5a, LB2a, LB262, LBB and LB1a that gave surface tension about 26 mN/m were identified as Bacillus sp. Natural manipueira (high solids content) and decanted manipueira (no solids) were investigated as culture media for biosurfactant production by selected microorganisms. Natural manipueira medium showed minimum surface tension of 28 mN/m (LB5a isolate) whereas for decanted manipueira the lowest value was 26 mN/m (isolate LB2a). Average diameter of growth on manipueira agar was 7.2 cm for isolate LB5a suggesting a high growth capacity on this substrate. Manipueira comprises a potential alternative culture medium for biosurfactant production by the selected isolates.

Propriedades emulsificantes e estabilidade do biossurfactante produzido por Bacillus subtilis em manipueira

“…O biossurfactante foi produzido por Bacillus subtilis linhagem LB5a, pertencente à coleção do Laboratório de Bioaromas (DCA/FEA/Unicamp) (NITSCHKE; FERRAZ;PASTORE, 2004). Foi realizada fermentação em fermentador piloto com capacidade para 80 L Pilot New Brunswick 8000 MP 80 (New Brunswick Scientific, Edison, NJ, EUA), utilizando-se manipueira pré-tratada, segundo Barros et al (2008), como substrato.…”

Surfactina: propriedades químicas, tecnológicas e funcionais para aplicações em alimentos

“…Ressalta-se que diferenças na composição do meio refletiram em diferenças nas curvas de tensão superficial 67 . Substratos adicionais foram sugeridos para produção de biossurfactante, tais como melaço e soro de leite 68 . Além desses resíduos citados, há descrição da utilização de outros resíduos, como rejeitos do processamento de óleos 69 e resíduos do processamento de soja 70 .…”

“…No caso da produção de biossurfactante, várias linhagens de Bacillus subtilis foram testadas segundo suas habilidades em utilizar a manipueira para produção de surfactina 21,37,68,73 . Quando comparada com meio sintético e com alguns resíduos, tais como melaço e soro de leite, segundo a tensão superficial final do meio de cultura, a manipueira foi o substrato no qual os menores valores de tensão foram encontrados.…”

Use of biosurfactant surfactin produced from cassava wastewater for anaerobic treatment of effluent from a poultry slaughterhouse

“…The microorganism that produces the biosurfactant, surfactin in cassava wastewater is the bacterium, B. subtilis LB5a, which was stored in agar nutrient at 4°C, at Bioaromas Laboratory of FEA/UNICAMP (Nitschke et al, 2004). Cropping of this microorganism for biosurfactant production in cassava was carried out in a pilot bioreactor, New Brunswick Scientific ® , model Mobile Pilot Plant fermentor 8000 MP 80 with 56 L of cassava wastewater as growth medium and 4 L of inoculum.…”

“…Cropping of this microorganism for biosurfactant production in cassava was carried out in a pilot bioreactor, New Brunswick Scientific ® , model Mobile Pilot Plant fermentor 8000 MP 80 with 56 L of cassava wastewater as growth medium and 4 L of inoculum. The experimental conditions of such bioreactor, procedures for obtaining semipurification of biosurfactant, are described by Nitschke and Pastore (2003), Nitschke et al (2004) and Barros et al (2008).…”