Selection of High Affinity Peptides for Prediction of Colorectal Adenoma-to-Carcinoma Progression

Hwang, Hye Jin; Ryu, Myung Yi; Lee, Gyu Bum; Park, Jong Pil

doi:10.1002/slct.201600173

Cited by 10 publications

(5 citation statements)

References 21 publications

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“…Using this approach, our group recently reported a new electrochemical phage-based sensor for the detection of various targets [37–40]. Therefore, free peptides, not associated with whole M13 phages, may utilize as alternative recognition reagent for the creation of a peptide-decorated electrochemical sensor for the early diagnosis of virus infection or target of interest [38, 39]. Therefore, affinity peptides can act as specific recognition element over antibodies in point-of-care testing because of their structural versatility, specificity and high binding affinity for targets [40].…”

Section: Introductionmentioning

confidence: 99%

Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

et al. 2019

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Dengue virus (DENV) nonstructural 1 (NS1) protein is a specific and sensitive biomarker for the diagnosis of dengue. In this study, an efficient electrochemical biosensor that uses chemically modified affinity peptides was developed for the detection of dengue virus NS1. A series of amino acid-substituted synthetic peptides was rationally designed, chemically synthesized and covalently immobilized to a gold sensor surface. The sensor performance was monitored via square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Potential affinity peptides specific for NS1 were chosen according to the dynamic current decrease in SWV experiments. Using circular dichroism, the molar ellipticity of peptides (DGV BP1–BP5) was determined, indicating that they had a mostly similar in random coil structure, not totally identical. Using SWV, DGV BP1 was selected as a promising recognition peptide and limit of detection for NS1 was found to be 1.49 μg/mL by the 3-sigma rule. DGV BP1 showed good specificity and stability for NS1, with low signal interference. The validation of the sensor to detect NS1 proteins was confirmed with four dengue virus culture broth (from serotype 1 to 4) as proof-of-concept. The detection performance of our sensor incorporating DGV BP1 peptides showed a statistically significant difference. These results indicate that this strategy can potentially be used to detect the dengue virus antigen, NS1, and to diagnosis dengue fever within a miniaturized portable device in point-of-care testing.

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Section: Introductionmentioning

confidence: 99%

Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

et al. 2019

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“…A new approach for the early diagnosis of CRC in plasma samples from patients with adenomas and carcinomas used synthetic affinity peptides identified by the phage display technique [145]. Synthetic peptides were selectively bound to the leucine-rich α-2-glycoprotein 1 (LRG1) that was overexpressed in CRC patients, and the level of plasma LRG1 was related to the progression of CRC from the adenoma stage to carcinoma [146]. Four series of specific peptides that recognize the LRG1 protein were synthesized to be different in some amino acids and modified with a C-terminal cysteine and a flexible connector (-GGGGS-) to form a SAM on gold electrodes.…”

Section: Electrochemical Biosensing Of Protein Biomarkers Of Crcmentioning

confidence: 99%

Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers

et al. 2020

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The accurate determination of specific tumor markers associated with cancer with non-invasive or minimally invasive procedures is the most promising approach to improve the long-term survival of cancer patients and fight against the high incidence and mortality of this disease. Quantification of biomarkers at different stages of the disease can lead to an appropriate and instantaneous therapeutic action. In this context, the determination of biomarkers by electrochemical biosensors is at the forefront of cancer diagnosis research because of their unique features such as their versatility, fast response, accurate quantification, and amenability for multiplexing and miniaturization. In this review, after briefly discussing the relevant aspects and current challenges in the determination of colorectal tumor markers, it will critically summarize the development of electrochemical biosensors to date to this aim, highlighting the enormous potential of these devices to be incorporated into the clinical practice. Finally, it will focus on the remaining challenges and opportunities to bring electrochemical biosensors to the point-of-care testing.

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“…ELISA may also be applied to study affinity between target and phage-displayed ligand (e.g. peptide) [13][14][15][16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

Determination of the Dissociation Constant for Polyvalent Receptors using ELISA: a Case of M13 Phages Displaying Troponin T-specific Peptides

Machera

Niedziółka‐Jönsson

Jönsson-Niedziółka

et al. 2023

Preprint

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Phage-derived affinity peptides have become widespread thanks to their easy selection via phage display. Interactions between a target protein and its specific peptide are similar to those between antibodies and antigens. The strength of these non-covalent complexes may be described by the dissociation constant (Kd). In this paper, protein-specific peptides are exposed on the pIII protein present in the M13 bacteriophage virion with up to 5 copies. Therefore, one phage particle can bind from one to five ligands. Here, we discuss the dependences between phage-displayed peptides and their ligands in solution using a model system based on troponin T (TnT) binding phages. Moreover, a method of calculating Kd values from ELISA experiments was developed and presented. Determined Kd values are in the picomolar range.

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Selection of High Affinity Peptides for Prediction of Colorectal Adenoma-to-Carcinoma Progression

Cited by 10 publications

References 21 publications

Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers

Determination of the Dissociation Constant for Polyvalent Receptors using ELISA: a Case of M13 Phages Displaying Troponin T-specific Peptides

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