Selection of high affinity aptamer-ligand for dexamethasone and its electrochemical biosensor

Mehennaoui, Somia; Poorahong, Sujittra; Jiménez, Gastón Contreras

doi:10.1038/s41598-019-42671-3

Cited by 38 publications

(29 citation statements)

References 27 publications

(29 reference statements)

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“…Figure 3a-c show the AFM images of the proposed electrode, after each step of the functionalization. As can be seen, after the adsorption of the PBSE and the immobilization of the aptamers, the roughness of the electrode surface is decreasing, which is in good agreement with other similar reports using aptamers for the electrode functionalization 48 . The formation of a complete layer of the PBSE and the immobilization of a monolayer of the aptamers on top of the graphene cause the significant decrease in the roughness of the electrode.…”

Section: Resultssupporting

confidence: 91%

Extended gate field-effect-transistor for sensing cortisol stress hormone

et al. 2021

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Cortisol is a hormone released in response to stress and is a major glucocorticoid produced by adrenal glands. Here, we report a wearable sensory electronic chip using label-free detection, based on a platinum/graphene aptamer extended gate field effect transistor (EG-FET) for the recognition of cortisol in biological buffers within the Debye screening length. The device shows promising experimental features for real-time monitoring of the circadian rhythm of cortisol in human sweat. We report a hysteresis-free EG-FET with a voltage sensitivity of the order of 14 mV/decade and current sensitivity up to 80% over the four decades of cortisol concentration. The detection limit is 0.2 nM over a wide range, between 1 nM and 10 µM, of cortisol concentrations in physiological fluid, with negligible drift over time and high selectivity. The dynamic range fully covers those in human sweat. We propose a comprehensive analysis and a unified, predictive analytical mapping of current sensitivity in all regimes of operation.

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Section: Resultssupporting

confidence: 91%

Extended gate field-effect-transistor for sensing cortisol stress hormone

et al. 2021

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“…The fluorophore-labeled dexamethasone-aptamer (Flu-DEX-apt) is composed of 59 nucleotides. Dexamethasone-aptamer was labeled with fluorescein isothiocyanate (FITC) fluorophore: Fluor: 5’-/5FluorT/AC-ACG-ACG-AGG-GAC-GAG GAG-TAC-TTG-CCA-ACG-ATA-ACG-TCG-TTG-GAT-CTG-TCT-GTG-CCC-3’ [ 21 ]. Different hormones i.e., 19-norethindrone, estrone, dexamethasone, progesterone, and estradiol were purchased from Sigma-Aldrich (Oakville, ON, Canada).…”

Section: Methodsmentioning

confidence: 99%

“…Herein, we reported the synthesis of a stable, biocompatible and robust 3D porous rGO/PEI/Flu-DEX-apt membrane [ 20 , 21 ]. The prepared rGO/PEI/Flu-DEX-apt foam exhibited excellent stability in the solution.…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide Membrane Immobilized Aptamer as a Highly Selective Hormone Removal

et al. 2020

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Three-dimensional (3D) reduced graphene oxide (rGO) modified by polyethyleneimine (PEI) was prepared and functionalized by fluorophore-labeled dexamethasone-aptamer (Flu-DEX-apt) via π–π stacking interaction. The rGO/PEI/Flu-DEX-apt was used as a selective membrane for dexamethasone hormone removal from water. The prepared rGO/PEI/Flu-DEX-apt membranes were stable, insoluble, and easily removable from liquid media. The membrane was characterized by Raman spectroscopy, scanning electron spectroscopy, and FTIR spectroscopy. The rGO/PEI/Flu-DEX-apt membrane showed high sensitivity and specificity toward the dexamethasone hormone in the presence of other steroid hormone analogs, such as progesterone, estrone, estradiol, and 19-norethindrone. The fluorescence and UV–visible spectroscopy were used to confirm the membranes performance and the quantification of hormones removal. The resulting data clearly show that the graphene oxide concentration influence the aptamers and analytes interaction (π–π stacking interaction). It was found that by varying the graphene oxide concentration yields to different porosities of rGO/PEI/Flu-DEX-apt membranes affects the adsorption recovery rate, as well as the specificity and selectivity toward the dexamethasone hormone.

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“…The cross-reactivity of antibodies was found between milk proteins (only caseins and β-LG) of cow and others (i.e., ewe, goat, and buffalo) but not with camel's milk proteins [56]. In comparison to antibodies, the choice of specific aptamers can resolve the issue of cross-reactivity with other proteins of similar pattern [57]. In this section, both immunosensors and aptamer-based biosensors have been considered for the milk allergens detection (refer to Table 3).…”

Section: Biosensors In Detecting Food Allergensmentioning

confidence: 99%

Nano-Biosensing Platforms for Detection of Cow’s Milk Allergens: An Overview

Nehra

Lettieri

Dilbaghi

et al. 2019

Sensors

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Among prevalent food allergies, cow milk allergy (CMA) is most common and may persist throughout the life. The allergic individuals are exposed to a constant threat due to milk proteins’ presence in uncounted food products like yogurt, cheese, and bakery items. The problem can be more severe due to cross-reactivity of the milk allergens in the food products due to homologous milk proteins of diverse species. This problem can be overcome by proper and reliable food labeling in order to ensure the life quality of allergic persons. Therefore, highly sensitive and accurate analytical techniques should be developed to detect the food allergens. Here, significant research advances in biosensors (specifically immunosensors and aptasensors) are reviewed for detection of the milk allergens. Different allergic proteins of cow milk are described here along with the analytical standard methods for their detection. Additionally, the commercial status of biosensors is also discussed in comparison to conventional techniques like enzyme-linked immunosorbent assay (ELISA). The development of novel biosensing mechanisms/kits for milk allergens detection is imperative from the perspective of enforcement of labeling regulations and directives keeping in view the sensitive individuals.

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Selection of high affinity aptamer-ligand for dexamethasone and its electrochemical biosensor

Cited by 38 publications

References 27 publications

Extended gate field-effect-transistor for sensing cortisol stress hormone

Extended gate field-effect-transistor for sensing cortisol stress hormone

Graphene Oxide Membrane Immobilized Aptamer as a Highly Selective Hormone Removal

Nano-Biosensing Platforms for Detection of Cow’s Milk Allergens: An Overview

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