Selection of DNA aptamers against Human Cardiac Troponin I for colorimetric sensor based dot blot application

Horseradish peroxidase-triggered direct in situ fluorescent immunoassay platform for sensing cardiac troponin I and SARS-CoV-2 nucleocapsid protein in serum

“…5 C). The detection limit was 0.19 ng/mL, as calculated from 3σ/S, which was superior to those previously reported assay for cTnI detection ( Table S3 ) ( Liu et al, 2016 ; Wu et al, 2010 , 2017 ; Guo et al, 2019 ; Song et al, 2011 ; Dorraj et al, 2015 ; Zuo et al, 2016 ; Tsaloglou et al, 2014 ; Periyakaruppan et al, 2013 ). Comprehensive consideration of performance, cost, and detection time, our method still showed obvious overall advantages compared with other fluorescence-based methods ( Liu et al, 2020 , Liu et al, 2020 , Liu et al, 2020 ; Jarvenpaa et al, 2012 ; Zhao et al, 2019 ; Liu et al, 2020 , Liu et al, 2020 , Liu et al, 2020 ) ( Table S4 ).…”

Essential strategies to optimize asymmetric PCR conditions as a reliable method to generate large amount of ssDNA aptamers

“…Complete removal of dsDNA is not possible in the asymmetric PCR and it is recommended that this technique be performed with a complementary method. For instance, treating the PCR product with low units of lambda exonuclease that digests the remaining dsDNA and leads to a higher purity of ssDNA .…”

A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I

“…Previously many methods have been used for cTnI detection and quantification, such as enzyme-linked immunosorbent assay (ELISA), 10 chemiluminescence immunoassays, 11,12 fluorescence immunoassays, [13][14][15] electrical detection, 16 surface plasmon resonance detection (SPR), 17,18 colorimetric protein array, 19 naked eye 20 and so on. However, the major limitation in currently used cTnI assays is low sensitivity and precision at the time of a patient's presentation, owing to a delayed increase in circulating levels of cardiac troponin.…”