A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I

Fu, Xiuli; Wang, Yunqing; Liu, Yongming; Liu, Huitao; Fu, Longwen; Wen, Jiahui; Li, Jingwen; Wei, Peng; Chen, Lingxin

doi:10.1039/c8an02022a

Cited by 110 publications

(68 citation statements)

References 43 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the dissolution of AgNPs, a greatly decreased SERS signal of GO was obtained [42]. A GO-AuNPs-based amplification SERS immunoassay for detection of cTnI was demonstrated in Figure 13 [44]. Antibody and Raman reporter-labeled AuNPs-functionalized GO were employed as both SERS nanotags and signal amplification carriers.…”

Section: Graphene-based Sers Biosensorsmentioning

confidence: 99%

See 1 more Smart Citation

Graphene-Based Biosensors for Detection of Biomarkers

2020

View full text Add to dashboard Cite

The development of biosensors with high sensitivity and low-detection limits provides a new direction for medical and personal care. Graphene and graphene derivatives have been used to prepare various types of biosensors due to their excellent sensing performance (e.g., high specific surface area, extraordinary electronic properties, electron transport capabilities and ultrahigh flexibility). This perspective review focuses on graphene-based biosensors for quantitative detection of cancer-related biomarkers such as DNA, miRNA, small molecules and proteins by integrating with different signal outputting approaches including fluorescent, electrochemistry, surface plasmon resonance, surface enhanced Raman scattering, etc. The article also discussed their challenges and potential solutions along with future prospects.

show abstract

Section: Graphene-based Sers Biosensorsmentioning

confidence: 99%

mentioning

confidence: 99%

Graphene-Based Biosensors for Detection of Biomarkers

2020

View full text Add to dashboard Cite

show abstract

“…Antibody/Raman reporters labeled AuNPs-functionalized GO were applied as both SERS nanotags and signal amplification carriers. Due to the strong SERS enhancement ability and the binding chance with cTnI of GO/AuNPs complexes, the proposed SERS-based immunoassay achieved a high sensitive LOD (5 pg mL −1 ) and a good linearity in a range of 0.01-1000 ng mL −1 [169] (Figure 12a). Chen et al constructed a high-sensitive and low-power theranostic nanosystem that combines with synergistic photothermal therapy and SERS mapping.…”

Section: Other Kind Of Sensorsmentioning

confidence: 99%

“…This research group also fabricated a similar AuNPs/PDA SPR immunosensor for cardiac biomarker troponin I (cTropI) with LOD of 38 ng mL −1 , Figure 12. (a) The SERS strategy based on GO/AuNPs signal amplification for quantification of cTnI [169]. (b) The SPR biosensor based MMWCNTs-PDA immune probe for the detection of cTnI [171].…”

Section: Other Kind Of Sensorsmentioning

confidence: 99%

Noble Metal Nanostructured Materials for Chemical and Biosensing Systems

et al. 2020

View full text Add to dashboard Cite

Nanomaterials with unique physical and chemical properties have attracted extensive attention of scientific research and will play an increasingly important role in the future development of science and technology. With the gradual deepening of research, noble metal nanomaterials have been applied in the fields of new energy materials, photoelectric information storage, and nano-enhanced catalysis due to their unique optical, electrical and catalytic properties. Nanostructured materials formed by noble metal elements (Au, Ag, etc.) exhibit remarkable photoelectric properties, good stability and low biotoxicity, which received extensive attention in chemical and biological sensing field and achieved significant research progress. In this paper, the research on the synthesis, modification and sensing application of the existing noble metal nanomaterials is reviewed in detail, which provides a theoretical guidance for further research on the functional properties of such nanostructured materials and their applications of other nanofields.

show abstract

“…To fabricate a stable metal-nanoparticle platform, graphene oxide (GO) was used as a support for the arrangement of AuNPs. Graphene and graphene-based substrates are considered a promising material for SERS sensors owing to their large surface area and excellent molecule adsorption ability, and therefore, a number of graphene-based SERS devices have been proposed [7,[18][19][20][21][22][23][24][25][26]. In addition, GO plays a crucial role as a fluorescence quencher and molecular stabilizer that render GO-metal NPs as hybrid platforms [27].…”

mentioning

confidence: 99%

Hydrophobic Paper-Based SERS Sensor Using Gold Nanoparticles Arranged on Graphene Oxide Flakes

Lee

Kim

2019

Sensors

View full text Add to dashboard Cite

Paper-based surface-enhanced Raman scattering (SERS) sensors have garnered much attention in the past decade owing to their ubiquity, ease of fabrication, and environmentally friendly substrate. The main drawbacks of a paper substrate for a SERS sensor are its high porosity, inherent hygroscopic nature, and hydrophilic surface property, which reduce the sensitivity and reproducibility of the SERS sensor. Here, we propose a simple, quick, convenient, and economical method for hydrophilic to hydrophobic surface modification of paper, while enhancing its mechanical and moisture-resistant properties. The hydrophobic paper (h-paper) was obtained by spin-coating diluted polydimethylsiloxane (PDMS) solution onto the filter paper, resulting in h-paper with an increased contact angle of up to ≈130°. To complete the h-paper-based SERS substrate, gold nanoparticles arranged on graphene oxide (AuNPs@GO) were synthesized using UV photoreduction, followed by drop-casting of AuNPs@GO solution on the h-paper substrate. The enhancement of the SERS signal was then assessed by attaching a rhodamine 6G (R6G) molecule as a Raman probe material to the h-paper-based SERS substrate. The limit of detection was 10 nM with an R2 of 0.966. The presented SERS sensor was also tested to detect a thiram at the micromolar level. We expect that our proposed AuNPs@GO/h-paper-based SERS sensor could be applied to point-of-care diagnostics applications in daily life and in spacecraft.

show abstract

A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I

Abstract: A multiple signal amplification of a SERS biosensor was developed for sensitive detection of cTnI with the aid of GO/AuNP complexes.

Cited by 110 publications

References 43 publications

Graphene-Based Biosensors for Detection of Biomarkers

Graphene-Based Biosensors for Detection of Biomarkers

Noble Metal Nanostructured Materials for Chemical and Biosensing Systems

Hydrophobic Paper-Based SERS Sensor Using Gold Nanoparticles Arranged on Graphene Oxide Flakes

Contact Info

Product

Resources

About