Selection of Bupleurum falcatum Callus Line Producing Anthocyanins in Darkness

Hiraoka, Noboru; Kodama, Tomoko; Tsutsumi, Yutaka

doi:10.1021/np50045a015

Cited by 13 publications

(4 citation statements)

References 7 publications

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“…When the culture period of suspension cultures was greater than 2 to 3 weeks, cellbrowning occurred by the formation of polyphenolics, causing cell death. Whereas the cell suspension cultures obtained from B. falcatum (Hiraoka et al 1986) and A. reptans (Callebaut et al 1990) were heterogeneous, it was confirmed by microscopic observations that the 0 0 anthocyanin-producing cells in A. cordata cell suspension cultures were highly homogeneous (anthocyanin-producing cells/total cells _->0.90).…”

Section: Growth and Production Of Anthocyanins In Callus Culturesmentioning

confidence: 84%

“…Anthocyanins, however, usually accumulate only in small amounts in cultured plant cells and their production generally requires light irradiation. Only a few plant cell cultures have been reported to produce anthocyanins in the dark, but the anthocyanin levels are very low: Ajuga reptans (Callebaut et al 1990), Daucus carota Kinnersley & Dougall 1980), Vitis hybrid (Yamakawa et al 1983) and Bupleurum falcatum (Hiraoka et al 1986). To date, no reports have appeared on the selection of high anthocyaninproducing cell lines grown in the dark.…”

Section: Introductionmentioning

confidence: 99%

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Anthocyanin production in cultured cells of Aralia cordata Thunb.

Sakamoto¹,

Iida²,

Sawamura³

et al. 1994

Plant Cell Tiss Organ Cult

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High anthocyanin-producing cell lines, which were grown in a dark or in a light-dark regime, were selected from callus cultures initiated from stem and leaf tissues of Aralia cordata Thunb. by small-cell-aggregate selection. To verify the optimum culture conditions for anthocyanin production, cells were tested by changing the various basal media, sucrose concentration and nitrogen source and concentration. Good growth was obtained in the dark on Linsmaier-Skoog's basal medium containing 1.0 mg 1-1 2,4-D and 0.1 mgl -~ kinetin, 2% (w/v) sucrose and full strength of nitrogen concentration. However, the highest anthocyanin yield (10.3% dry wt) was obtained in the dark on B5 medium containing 1.0 mg 1-1 2,4-D and 0.1 mg 1-1 kinetin. Our results suggested that it has became feasible to find the most effective conditions for cell growth and anthocyanin production by optimizations of the nitrogen concentration and the ratio of NH 4 to NO 3 in the medium.

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Section: Growth and Production Of Anthocyanins In Callus Culturesmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Anthocyanin production in cultured cells of Aralia cordata Thunb.

Sakamoto¹,

Iida²,

Sawamura³

et al. 1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…3. Whereas the cell suspension cultures obtained from BupleurumJalcatum (Hiraoka et al 1986) and Ajuga reptans (Callebaut et al 1990) were heterogeneous, it was confirmed by microscopic observations that the anthocyanin-producing cells in A. cordata cell suspension cultures were highly homogeneous (anthocyanin-producing cells/ total cells ;:::; 0.9). The growth of both cell lines attained a plateau after 4 weeks.…”

Section: Growth and Production Oj Anthocyanin In Callus Tzssuementioning

confidence: 82%

“…1. Only a few cell cultures have been reported to produce anthocyanins in the dark, but the anthocyanin levels are very low: Ajuga reptans (Callebaut et al 1990), Daucus carota Kinnersley and Dougall 1980), Vitis hybrid (Yamakawa et al 1983), and Bupleurum falcatum (Hiraoka et al 1986). Anthocyanins, however, usually accumulate only in small amounts in cultured cells and their production generally requires light irradiation.…”

Section: Studies On Anthocyanin Production Using Cell Culturementioning

confidence: 99%