Anthocyanin production in cultured cells of Aralia cordata Thunb.

Abstract

High anthocyanin-producing cell lines, which were grown in a dark or in a light-dark regime, were selected from callus cultures initiated from stem and leaf tissues of Aralia cordata Thunb. by small-cell-aggregate selection. To verify the optimum culture conditions for anthocyanin production, cells were tested by changing the various basal media, sucrose concentration and nitrogen source and concentration. Good growth was obtained in the dark on Linsmaier-Skoog's basal medium containing 1.0 mg 1-1 2,4-D and 0.1 mgl -~ kinetin, 2% (w/v) sucrose and full strength of nitrogen concentration. However, the highest anthocyanin yield (10.3% dry wt) was obtained in the dark on B5 medium containing 1.0 mg 1-1 2,4-D and 0.1 mg 1-1 kinetin. Our results suggested that it has became feasible to find the most effective conditions for cell growth and anthocyanin production by optimizations of the nitrogen concentration and the ratio of NH 4 to NO 3 in the medium.

Effects of macro elements and nitrogen source on biomass accumulation and bacoside A production from adventitious shoot cultures of Bacopa monnieri (L.)

“…This feature will certainly offer us a way to control the production of secondary metabolites in cultures. Many reports have confirmed that callus and root growth, as well as secondary metabolite accumulation are promoted under the influence of NO 3 -in cell cultures of W. somnifera and Aralia cordata (Sakamoto et al 1994). …”

Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels

“…It was hypothesized that the ratio of NH 4 ? to NO 3 -nitrogen improved anthocyanin production, which was mainly due to change in primary cellular metabolism (Yamakawa et al 1983;Sakamoto et al 1994;Sato et al 1996;KonczakIslam et al 2001;Nagira and Ozeki 2004).…”

Influence of auxins, cytokinins, and nitrogen on production of rutin from callus and adventitious roots of the white mulberry tree (Morus alba L.)

“…ratio in the medium was found to decrease the cellular syntheses of berberine in Thalictrum minus and ubiquinone in Nicotiana tabacum (Nakagawa et al 1984;Ikeda et al 1977) and of alkaloids in Atropa belladonna hairy roots (Bensaddek et al 2001). The total nitrogen content is also a contributing factor (Sakamoto et al 1994). The ammonium/nitrate ratio controls the pH of the growth media, stimulates morphogenesis and embryogenesis, and thus is important for inducing callus formation in many woody plant cultures.…”